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http://dx.doi.org/10.6114/jkood.2018.31.1.001

Effects of Shinsuwisaengtang on Antioxidant Activity and Inhibition of Phototoxicity by UVB Irradiation  

Kim, Ee-Hwa (Dept. of Meridian & Acupoint, College of Korean Medicine, Semyung University)
Kim, Yong-Min (Dept. of Cosmetic Science & Beauty Biotechnology, Semyung University)
Kim, Hee-Taek (Dept. of Korean Medical Ophthalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.31, no.1, 2018 , pp. 1-11 More about this Journal
Abstract
Objectives : The purpose of this study is to investigate the effect of Shinsuwisaengtang (SSWST) extracts on antioxidant activity and inhibition of phototoxicity as a medicine for skin damage due to ultra violet stimulation. Methods : To determine the cytotoxicity of Hs68 cells from SSWST extracts, we investigated cell viability by MTT assay. To determine the protective effect of phototoxicity, we investigated cell viability after UVB radiation. The DPPH radical scavenging activity was examined to determine the antioxidant effect of SSWST extracts. Hoechst 33258 staining was performed to observe the protective effect of SSWST on UVB. Results : The cytotoxicity of SSWST extracts in Hs68 cells was not appeared significantly in all concentration. SSWST extracts significantly increased the viability of UVB-stimulated Hs68 cells at a concentration of 25, 50 and $100{\mu}g/ml$. SSWST extract showed higher DPPH radical scavenging than the control group. It was observed that SSWST extracts inhibited the apoptosis of the UVB-stimulated Hs68 cells through a fluorescence microscope. Conclusions : It was observed that SSWST did not induce cytotoxicity at a constant concentration and had a protective effect on phototoxicity and an antioxidant effect. Thus, it is considered that it maybe used as a medicine to cure skin damage caused by UVB and photoaging changes in the future.
Keywords
Antioxidant; Photoaging; Phototoxicity; Shinsuwisaengtang; UVB;
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Times Cited By KSCI : 4  (Citation Analysis)
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