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http://dx.doi.org/10.6114/jkood.2016.29.4.024

Anti-Inflammatory Effects of Tongbi-san(通痺散) Extract on RAW264.7 Macrophages  

Kim, Yong-Min (School of Oriental Medicine and Bio Convergence Sciences, Semyung University)
Kim, Hee-Taek (Dept. of Korean Medical Ophthalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University)
Kim, Ee-Hwa (Dept. of Meridian & Acupoint, College of Korean Medicine, Semyung University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.29, no.4, 2016 , pp. 24-33 More about this Journal
Abstract
Objectives : This study is to investigate the anti-inflammatory and anti-oxidant effects of Tongbi-san extract (TS) on RAW264.7 macrophages using by cell cytotoxicity, Nitric Oxide (NO) and Prostaglandin $E_2$ ($PGE_2$) production and 1,1-diphenyl-2-picryl ghdrazyl (DPPH) free radical scavenging capability. Methods : Cell cytotoxicity was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The production of NO was measured by Griess assay. The production of $PGE_2$ was measured by immunoassay. And the anti-oxidant activity was measured by the DPPH method. Results : TS did not increased significantly compared to the TS untreated group in the cell cytotoxicity. TS inhibited NO and $PGE_2$ production in lipopolysaccharide-stimulated RAW 264.7 cells. TS had the DPPH free radical scavenging capability. Conclusion : The anti-inflammtory and anti-oxidant effects of TS may be use for a treatment of anti-inflammatory diseases.
Keywords
Anti-inflammation; Anti-oxidant activity; Nitric Oxide; Prostaglandin $E_2$; reactive oxygen species; Tongbi-san;
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1 Drobek-Slowik M, Karezewicz DK. The potential role of oxidative stress in the pathogenesis of the age-related macular degeneration (AMD). Postepy Hig Med Dosw. 2007;61:28-37.
2 Zafra-Stones S, Yasmin T, Bagchi M, Chattejee A, Vinson JA, Bagchi D. Berry anthocyanins as novel antioxidants in human health and disease prvention. Mol Nutr Food Res. 2007;51:675-83.   DOI
3 Tang Q, Chen L, Jie Y. Effects of traditional Chinese medicine Drynaria fortuneismith on promoting the proliferation, differentiation and calcification of mouse osteoblastic MC3T3-E1 cells. China Journal of Chinese Mareria Medica. 2004;29(2):64-168.
4 Cordell GA. Phytochemistry and traditional medicine-the revolution continues. Phytochem Lett. 2014;10:28-40.   DOI
5 World Health Organization. WHO Traditional medicine strategy. 2013. Availble from:URL: http://www.who.int/medicines/publications/traditional/trm-strategy14_23.
6 Torres-Rodrigues ML, Garcia-Chavez E, Berhow M, de Mejia EG. Anti-inflammatory and anti-oxidant effect of Calea urticifolia lyophilized aqueous extract on lipopolysaccharide-stimulated RAW 264.7 macrophages. J Ethnopharmacol. 2016;188:266-74.   DOI
7 Tracey KJ. The inflammatory reflex. Nature. 2002;420:853-9.   DOI
8 Nakagawa T, Yokozawa T. Direct scavenging of nitric oxide and superoxide by green tea. Food Chem. Toxicol. 2002;40:1745-50.   DOI
9 Chen X, Miao J, Wang H, Zhao F, Hu J, Gao P, et al. The anti-inflammatory activities of Ainsliaea fragrans Champ. extract and its components in lipopolysaccharide-stimulated RAW264.7 macrophages through inhibition of NF-${\kappa}B$ pathway. J Ethnopharmacol. 2015;170:72-80.   DOI
10 Devasagayam TPA, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD. Free radicals and antioxidants in human health: current status and future prospects. Journal of the Association of Physicians of India. 2004;52:794-804.
11 Kim HK, Cheon BS, Kim YH, Kim SY, Kim HP. Effects of naturally occurring flavonoids on nitric oxide production in the macrophage cell line RAW 264.7 and their structure-activity relationships. Biochem. Pharmacol. 1999;58:759-65.   DOI
12 Surh YJ, Chun KS, Cha HH, Han SS, Keum Y, Park K, et al. Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: downregulation of COX-2 and iNOs through suppression of $NF{\kappa}B$ activation. Mutat Res. 2001;480-481:243-68.   DOI
13 Tilg H, Moschen AR. Inflammatory mechanisms in the regulation of insulin resistance. Mol Med. 2008;14:222-31.
14 Chang YC, Li PC, Chen BC, Chang MS, Wang JL, Chiu WT, et al. Lipoteichoic acid-induced nitric oxide synthase expression in RAW 264.7 macrophages is mediated by cyclooxygenase-2, prostaglandin $E_2$, protein kinase A, p38 MAPK, and nuclear factor-kappa B pathways. Cell Signal. 2006;18:1235-43.   DOI
15 Zhang L. Zhang's Treatise on general medicine. 2nd ed. Beijing:Traditional Chinese Medical Publishing Company in China. 1995;809-10.
16 Lim HH, Kim YO, Seo MJ, Choi SW. Anti-Inflammatory Effects of Volatile Flavor Extract from Herbal Medicinal Prescriptions Including Cnidium officinale Makino and Angelica gigas Nakai. Journal of the society of cosmetics scientists of korea. 2011;37:199-210.
17 Han KS, Kim KC, Wang JH, Kim HJ. Effect of Unfermented and Fermented Atractylodes macrocephalae on Gut Permeability and Lipopolysaccharide-Induced Inflammation. Journal of Society of Korean Medicine for Obesity Research. 2013;13:24-32.
18 Cho JH, Kwon JE, Cho Y, Kim I, Kang SC. Anti-inflammatory effect of Angelica gigas via Heme Oxygenase-1 expression. Nutrients. 2015;15:4862-74.
19 Chen LG, Jan YS, Tsai PW, Norimoto H, Michihara S, Murayama C, et al. Antiinflammatory and Antinociceptive constituents of Atractylodes joponica koidzumi. J Agric Food Chem. 2016;64:2254-62.   DOI
20 Xu L, Kitade H, Ni Y, Ota T. Roles of chemokines and chemokine receptors in obesity-associated insulin resistance and nonalcoholic fatty liver disease. Biomolecules. 2015;5:1563-79.   DOI
21 Hotamisligil GS. Inflammation and metabolic disorders. Nature. 2006;444:860-7.   DOI
22 Reed GA, Arneson DW, Putnam WC, Smith HJ, Gray JC, Sullivan DK, et al. Single-dose and multiple-dose administration of indole-3-carbinol to women: pharmacokinetics based on 3,3'-diindolymethane. Cancer Epidermiol. Biomarkers Prev. 2006;15:2477-81.   DOI
23 Xia Z, Triffitt JT. A review on macrophage response to biomaterials. Biomed. Mater. 2006;1:1-9.   DOI
24 Ross JA, Auger MJ, Burke B, Lewis CE. The biology of the macrophage. In:Burker B, Lewis CE(Eds.). The Macrophage. 2nd ed. Oxford:Oxford Medical Publications. 2002:1-72.
25 Poltorak A, He X, Smirnova I, Liu MY, Van Huffel C, Du X, et al. Defective LPS signaling in C3H/Hej and C57BL/10ScCr mice. Mutations in Tlr4 gene. Science. 1998;282;2085-8.   DOI
26 Schmidt HH, Walter U. NO at work. Cell. 1994;78:919-25.   DOI
27 Hu XD, Yang Y, Zhong XG, Zhang XH, Zhang YN, Zheng ZP, et al. Antiinflammatory effects of Z23 on LPS-induced inflammatory responses in RAW264.7 macrophages. Journal of Ethnopharmacology. 2008;120:447-51.   DOI
28 St Clair EW, Wilkinson WE, Lang T, Sanders L, Misukonis MA, Gilkeson GS, et al. Increased expression of blood mononuclear cell nitric oxide synthase type 2 in rheumatoid arthritis patients. Journal of Experimental Medicine. 1996;184:1173-8.   DOI
29 Worrall NK, Lazenby WD, Misko TP, Lin TS, Rodi CP, Manning PT, et al. Modulation of in vivo alloreactivity by inhibition of inducible nitric oxide synthase. Journal of Experimental Medicine. 1995;181:63-70.   DOI
30 Chiou WF, Chou CJ, Chen CF. Camptothecin suppresses nitric oxide biosynthesis in RAW 264.7 macrophages. Life Sciences. 2001;69:625-35.   DOI
31 Bak MJ, Truong BL, Kang HS, Jun M, Jeong WS. Anti-inflammatory effect of procyanidins from wild grape (Vitis amurensis) seeds in LPS-induced RAW264.7 cells. Oxid Med Cell Longev. 2013:36;340-8.
32 Parthasarathy S, Khan-Marchant N, Penumeteha M, Santanam N. Oxidative stress in cardiovascular disease. J Nuclear Cardiol. 2001;8(3):379-89.   DOI
33 Benedetti S, Benvenah F, Pagharami S, Francegh S, Stephano S, Canestrari F. Antioxidant properties of a novel phycocyania extract from the blue-green alga Aphanizomenonflos-aquae. Life sci. 2004;75:2353-62.   DOI
34 Guerci B, Bohme P, Kearney-Schwartz A, Zannad F, Drouin P. Endothelial dysfunction and type 2 diabetes. Diabetes Metab. 2001;27:436-47.
35 Pincemail J, Meurisse M, Limet R, Defraigne JO. L'evaluation du stress oxydatif d'un individu : une realite pour le medecin. Vaisseaux Coeur Poumons. 1999;4(5):12-23.