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http://dx.doi.org/10.6114/jkood.2015.28.4.092

Studies on Immunoregulatory Effects of Bowon-tang in the Immune Cells  

Hwang, Joo-Min (Da Jung Korea Medicine Clinic)
Jung, Myung (Professional Graduate School of Korean Medicine, Wonkwang University)
Cho, Jung-Hoon (College of Korean Medicine Wonkwang University)
Lim, Kyu-Sang (Professional Graduate School of Korean Medicine, Wonkwang University)
Yun, Young-Gab (College of Korean Medicine Wonkwang University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.28, no.4, 2015 , pp. 92-110 More about this Journal
Abstract
Objectives : The water extract of Bowon-tang composited with thePanax, AstragalusandGlycyrrhiza Radixhas been traditionally used for treatment of a sickly child and smallpox in oriental medicine. However, little is known about the regulatory effects of Bowon-tang on the production, expression and activity of immune mediators [nitric oxide, prostaglandin E2, inducible nitric oxide synthetase, cyclooxygenase-2], the macrophage activation factor production, the proliferation, subset expression, the killing activity, and the capping in immune cells.Methods : In this study, we investigated the effects of water extracts from Bowon-tang,Panax, AstragalusorGRin mouse immune cells or human Jurkat T cells. Each extract (25-200 ㎍/㎖)perse had no cytotoxic effect in unstimulated macrophages, but concentration-dependently regulated NO and PGE2production, iNOS expression, and COX-2 activity in mouse peritoneal macrophages with MAF stimulation. These regulatory effects were synergistically increased by their combination (Bowon-tang).Results : The extract of Bowon-tang concentration-dependently regulated T cell proliferation, CD4+and CD8+expression, and NK killing activity in mouse splenocytes and capping in Jurkat T cells.Conclusions : These results suggest that the water extract of Bowon-tang composited with thePanax, AstragalusandGRmay be useful for therapeutic drugs against a sickly constitution and immune diseases, probably by regulating the production of immune mediators.
Keywords
Bowon-tang; prostaglandin E2 (PGE2); nitric oxide synthetase (iNOS); cyclooxygenase-2 (COX-2); macrophage activation factor (MAF);
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