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http://dx.doi.org/10.6114/jkood.2015.28.2.001

Study on the Anti-aging Activity of Gentianae sino-ornata Ethanol Extract  

Choi, Hyung-Wook (Professional Graduate School of Oriental Medicine, Wonkwang University)
Li, Shun-Hua (Department of Beauty Education, Graduate School of Education, Konkuk University)
Kim, Eun-Joo (Department of Beauty Health Design, Open Cyber University)
Kim, Soo-Kyung (Department of Beauty and Art, Suwon Women's College)
Lee, Jang-Cheon (Professional Graduate School of Oriental Medicine, Pusan National University)
Lim, Kyu-Sang (Professional Graduate School of Oriental Medicine, Wonkwang University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.28, no.2, 2015 , pp. 1-12 More about this Journal
Abstract
Objective : The goal of this study is to identify the effects of extract ofGentianae sino-ornata(GSO) on the anti-oxidative activity of skin.For this purpose, several functions of GSO were analyzed in terms of skin-lightening activity and wrinkle improvement. Methods : Cell viability was measured by neutral red (NR) assay, and GSO showed highly efficacy in DPPH radical scavenging activity. The level of tyrosinase and matrix metalloproteinase-1 (MMP-1) in media was analyzed by ELISA kit, and the expressions of p-JNK and p-ERK was measured by Western blot. To elucidate inhibitory effects of GSO on melanin synthesis, I determined the tyrosinase activity and melanin production in B16F10 cells. Results : MMP-1 production in UVB-stimulated HDF cells was inhibited by GSO treatments, and also GSO inhibited protein expression levels of p-JNK and p-ERK. GSO significantly reduced tyrosinase activity and melanin synthesis in B16F10 cells. Conclusions : From these results, GSO appears to be effective on skin elasticity increase, wrinkle improvement, whitening as anti-aging activity.
Keywords
ERK; JNK; Gentuanae sino-ornata; MMP-1; melanin; tyrosinase; $\alpha$-MSH;
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1 Jeun JA, Lee SJ. Application of Nurigenomics in the Beauty Foods Research. Food Sci Ind. 2007;40(4):27-30.
2 Pyo YH, Son JH, Choe TB. The Effect of Celosia cristata L. ethanol Extract on Anti-oxidant & Anti-aging Activity. Korean J. Biotechnol. Bioeng. 2008;23(5):431-8.
3 Lee JG, Kim EW, Lee JH. Effect of Gentianae Radix on Neurogenesis and Apoptosis in Hippocampus of Ethanol-induced Newborn Rats. J Oriental Neuropsychiatry. 2010;21(2):29-44.
4 Yang HJ, Won BR, Lim YJ, Yoon SK, Ji DH et al. Antioxidative Activity, Component Analysis, and Anti-elastase Effect of Aspalathus linearis Extract. J. Soc. Cosmet. Scientists Korea. 2007;33(4):251-62.
5 Oh SJ. Aging of Human Body. Tamkudang, Seoul, Korea, 2005;204-5.
6 Slominski A, Desmond J, Shibahara S, Wortsman J. Melanin pigmentation in mammalian skin and its hormonalregulation. Journals of the American Physiological Society. 2004;84(4):1155-228.
7 Park JH. An illustrated book of medicinal plants. Shinil company. Seoul. 2000; 306-8.
8 Kim CM, Shin MK, Ann DG, Lee CS. Great dictionary of Chinese Herb, Jhungdam company. Seoul. 1998;7:3178.
9 Borenfreund E, Puerner JA. Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol Lett. 1984;24(2-3):119-24.   DOI
10 Blois MS. Anti oxidant deter minations by the use of stable free radical. Nature. 1958;18:1199-20.   DOI
11 Lim HW, Yoon MY, Cha SB, Kim KW, Park YK, Lee JY. Effects of citrus essential oils melanin production in B16 melanima cell. J Yakhak Hoeji. 2003;47:15-30.
12 Homebeck W. Downregulation of tissue inhibitor of matrix metallo protease-1(TIMP-1) in aged human skin contributes to matrix degradation and impaired cell growth and survival. J Pathol Biol. 2003;51(10):569.   DOI
13 Kubo M, Matsuda H. Development tudies of cuticle and medicinal drug from natural sources on melanin biosynthesis. Fragrance J. 1995;8:48-55.
14 W laschej M, Brivib K, Stricjlin GP, Sies H,Scharffetter-Kochanek K. Singlet oxygen may ediate the ultraviolet A in induced synthesis of interstitial collagenase. J. Invest. Dermatol. 1995;104:194.   DOI
15 Pratt DE, Birac PM. Source of antioxidant activity of soybeans and soy products. J Food Sci. 1979;44:1720-28.   DOI
16 Ryu IS, Lee SJ, Lee SW, Mun YJ, Woo WH, Kim YM et al. Dermal Bioactive Properties of the Ethanol Extract from Flowers of Lespedeza bicolor. J Korean Med Ophthalmol Otolaryngol Dermatol. 2007;20(2):1-9.
17 No ST. Study of Skin Depigmenting Mechanism of the Ethanol Extract of Fagopyrum esculentum. Wonkwang University. 2006.
18 Won OY. Efficacy Evaluation of Deramal Bioactive Properties of the Chealbakgo Extract. Kwangju Women's University. 2014.
19 Haratake A, Uchida Y, Schmuth M. UVB-induced alterations in permeability barrier function: roles for epidermal hyperprolifera-tion and thymocyte-mediated response. J Invest Dermatol. 1998;108:769-75.   DOI
20 Homebeck W. Downregulation of tissue inhibitor of matrix metallo protease-1(TIMP-1) in aged human skin contributes to matrix degradation and impaired cell growth and survival. J Pathol Biol. 2003;51(10):569.   DOI
21 Do JR, Baek SY, Im SD, Kim YM, Kim IH, Han DS et al. Selection of beauty material from Fruits and Vegetables. J Kor Soc Food Sci. Nutr. 2008;10:248.
22 Zague V. A new view concerning the effects of collagen hydrolysate intake on skin properties. Arch. Dermatol. Res. 2008;300:479-83.   DOI
23 Inouye H, Nakamura Y. Studies on monoterpene glucosides and related natural products. XVI. Occurrence of secoiridoid glucosides in gentianaceous plants especially in the genera Gentiana and Swertia. Yakugaku Zassh. 1971;91(7):755.   DOI
24 Brinckmann J, Acil Y, Wolff HH, Muller PK. Collagen synthesis in (sun-) aged human skin and in fibroblasts derivedf rom sun-exposed and sin-protected body sites. Photochem Photobiol B. 1995;27:33-8.   DOI
25 Chang YH, Kim C, Jung M, Lim YH, Lee S, Kang S. Inhibition of melanogenesis by selina-4(14), 7(11)-dien-8-one isolated from Atractylodis Rhizoma Alba. Biol Pharm Bull. 2007;30:719-23.   DOI
26 Hearing VJ, Jimnez M. Mammalian tyrosinase the critical regulatory control point in melanocyte pigmentation. Int J Biochem. 1987;19:1141-7.   DOI
27 Cabanes J, Chazarra S, Garcia-Carmona F. Kojic acid, a cosmetic skin whitening agent, is a slow-binding inhibitor of catecholase activity of tyrosinase. J Pharm Pharmacol. 1994;46:982-5.   DOI
28 Seo CW. Studies on Antioxidant and Whitening Efficacy of Extracts from Smilax china L. by Supercritical CO2. Ajou University. 2006.