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http://dx.doi.org/10.6114/jkood.2015.28.1.041

Inhibitory Effect of the Ethanol Extract of Rosae rugosae Flos on the Hyperpigmentation and its Action Mechanism Induced by α-MSH  

Lee, Jin-Ho (BK21-plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University)
In, Myung-Hee (BK21-plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University)
Kang, Suk-Hoon (Dept. of Oriental Pharmacy, College of pharmacy, Wonkwang Oriental Medicines Research Institute, Wonkwang University)
Mun, Yeun-Ja (BK21-plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University)
Woo, Won-Hong (Dept. of Anatomy, College of Oriental Medicine, Wonkwang University)
Lim, Kyu-Sang (Research Center of Traditional Korean Medicine, Wonkwang University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.28, no.1, 2015 , pp. 41-52 More about this Journal
Abstract
Objective : This study investigated the inhibitory mechanism of the hypopigmentating effects on ethanol extract of Rosae rugosae Flos (ERR) that has not yet been examined. Methods : We analyzed the anti-melanogenic effects of ethanol extracts from Rosae rugosae Flos by tyrosinase activity, melanin contents. We also examined protein expression levels of tyrosinase, TRP-1, TRP-2, MITF and ERK by western blot analysis in melanoma cells. Results : In this investigation, ERR effectively reduced ${\alpha}$-MSH-stimulated melanin synthesis by suppressing expression of tyrosinase and tyrosinase-related protein-1 (TRP-1). On the other hand, the expression of tyrosinase-related protein-2 (TRP-2) were not affected by treatment with ERR. ERR inhibited the expression of microphthalmia-associated transcription factor (MITF) as a key transcription factor for tyrosinase expression regulating melanogenesis. The upstream signaling pathway including cAMP response element-binding protein (CREB) and MAPKs were also inhibited by ERR. Pretreatment with PD98059, ERK inhibitor, attenuated the inhibitory effect of ERR on ${\alpha}$-MSH-induced tyrosinase activity. Conclusions : Our study suggested that the anti-melanogenic activity of ERR is correlated with the suppression of tyrosinase gene through CREB/MITF/ERK pathway.
Keywords
Ethanol extract Rosae rugosae Flos (ERR); Melanin; Tyrosinase; Microphthalmia-associated transcription factor (MITF); Extracellular signal-regulated kinase (ERK);
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