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http://dx.doi.org/10.6114/jkood.2013.26.4.070

Anti-Inflammatory Effects of Haliotidis Concha  

Moon, Soo-Young (Medical Research Center (MRC-GHF), College of Oriental Medicine, Department of Herbal Formula, Daegu Haany University)
Kim, Young-Woo (Medical Research Center (MRC-GHF), College of Oriental Medicine, Department of Herbal Formula, Daegu Haany University)
Kim, Sang-Chan (Medical Research Center (MRC-GHF), College of Oriental Medicine, Department of Herbal Formula, Daegu Haany University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.26, no.4, 2013 , pp. 70-80 More about this Journal
Abstract
Objectives : Haliotidis Concha has been used to treat various human diseases such as liver dysfunction and inflammatory disorder. Although it has been shown the effects of Haliotidis Concha on the various diseases, it has almost not been studied about the anti-inflammatory effects of the Haliotidis Concha and its mechanisms. Methods : This research investigated the effects of the Haliotidis Concha ethanol extract (HCE) on the production of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) as well as tumor necrosis factor-alpha (TNF-${\alpha}$). The protein expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were assayed by immunoblot analyses, and the productions of NO, $PGE_2$ and TNF-${\alpha}$ were assessed by ELISA. Results : Haliotidis Concha decreased the production of NO and $PGE_2$, and inhibited the expression iNOS and COX-2 proteins in a concentration-dependent manner in LPS-treated Raw 264.7 cells. HCE suppressed the ability of LPS to activate the signaling pathways of nuclear factor kappa B (NF-${\kappa}B$) as indicated by HCE inhibited nuclear NF-${\kappa}B$ level and I-${\kappa}B{\alpha}$ phosphorylation. Also, HCE inhibited mitogen-activated protein kinases (MAPKs). Conclusions : HCE repressed the production of LPS-inducible NO, $PGE_2$ and TNF-${\alpha}$, which may be mediated by inhibition of NF-${\kappa}B$ translocation. This study suggest the use for the treatment of acute inflammatory disorders.
Keywords
Haliotidis Concha; LPS; iNOS; COX-2; Nuclear factor-${\kappa}B$;
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Times Cited By KSCI : 4  (Citation Analysis)
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