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http://dx.doi.org/10.6114/jkood.2011.24.1.078

The effects of Hyunggaeyungyo-tang of suppression of iNOS production on RAW 264.7cell  

Park, Jung-Hoon (동국대학교 대학원 한의학과 안이비인후피부과학교실)
Kim, Jong-Che (동국대학교 대학원 한의학과 안이비인후피부과학교실)
Hong, Seung-Ug (동국대학교 대학원 한의학과 안이비인후피부과학교실)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.24, no.1, 2011 , pp. 78-85 More about this Journal
Abstract
Background and Objectives : The aim of this study was to investigate anti-inflammatory and anti-oxidant effects of Hyunggaeyungyo-tang(HYT) on RAW 264.7 cells. Material and Methods : Two types of experiments were implemented for this study: first, the experiment to study the anti-oxidant effect of HYT using riboflavin; second, in vitro experiment to investigate the suppression of NF-${\kappa}$B activation using RAW 264.7 cells (I${\kappa}$B kinase and induce nitric oxide synthase mRNA expression) Results : 1. The anti-oxidant effects of HYT was dose-dependantly increased. 2. The RAW 264.7cells were treated with LPS for 1 hours prior to the addition of indicated concentrations(0.4,-1.0mg/$m\ell$) of HYT, and the cells were further incubated for 24 hours. The LPS-induced IKK & iNOS mRNA expression were dose-dependantly decreased in HYT treated RAW 264.7cells. Conclusion : The results suggest that HYT is significantly effective in the treatment of inflammation through the suppression of NF-${\kappa}$B activation and iNOS production.
Keywords
Hyunggaeyungyo-tang; anti-inflammation; NF-${\kappa}$B; iNOS;
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Times Cited By KSCI : 5  (Citation Analysis)
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