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http://dx.doi.org/10.6114/jkood.2011.24.1.025

Inhibitory Effect of Belamcandae Rhizoma on the Melanogenesis in MSH-stimulated B16F10 cells  

Kim, Dae-Sung (원광대학교 한의학전문대학원 한약자원개발학과)
Sung, Byung-Gon (원광대학교 한의학전문대학원 한약자원개발학과)
Lee, Jang-Cheon (부산대학교 한의학전문대학원 약물학부)
Lee, Boo-Kyun (세명대학교 본초방제학교실)
Woo, Won-Hong (원광대학교 한의학전문대학원 한약자원개발학과)
Lim, Kyu-Sang (원광대학교 한의학전문대학원 한약자원개발학과)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.24, no.1, 2011 , pp. 25-35 More about this Journal
Abstract
Objective : The present study was designed to assess the potential inhibitory activity of an ethanol extract of Belamcandae Rhizoma (EBR) on the alpha-melanocyte stimulating hormone (${\alpha}$-MSH)-induced melanogenesis signal pathway in B16F10 melanoma cells. Methods : Several experiments were performed in B16F10 melanoma cells. We studied tyrosinase activity, melanin content, cell-free tyrosinase activity and DOPA stain, and performed Western blots and RT-PCR for proteins and mRNA involved in melanogenesis. Results : ${\alpha}$-MSH-induced tyrosinase activity and melanin content were inhibited significantly by EBR. EBR markedly suppressed the protein expression level of tyrosinase in B16F10 melanoma cells. On the other hand, the expression of tyrosinase-related protein-1 (TRP-1) and -2 (TRP-2; DCT) were not affected by EBR. To elucidate the mechanism of the depigmenting property of EBR, we examined the involvement EBR in cAMP response element binding (CREB) protein phosphorylation and microphthalmia-associated transcription factor (MITF) signalling induced by ${\alpha}$-MSH. EBR did not regulate CREB phosphorylation and MITF expression by ${\alpha}$-MSH. Nevertheless, the mRNA expression of tyrosinase was significantly attenuated by EBR treatment without changes in the expression of TRP-1 and -2 mRNA. Conclusion : Our study suggested that EBR inhibits ${\alpha}$-MSH-induced melanogenesis by suppressing tyrosinase mRNA.
Keywords
Belamcandae Rhizoma; melanin; tyrosinase; ${\alpha}$-MSH;
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Times Cited By KSCI : 5  (Citation Analysis)
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