Browse > Article

Effects of Thujae Orientalis Folium (TOF) on Gene Expression of Human melanoma cells (SK-MEL-2)  

Jung, Min-Young (동신대학교 한의과대학 안이비인후피부과학교실)
Kim, Jong-Han (동신대학교 한의과대학 안이비인후피부과학교실)
Park, Su-Yeon (동신대학교 한의과대학 안이비인후피부과학교실)
Choi, Jeong-Hwa (동신대학교 한의과대학 안이비인후피부과학교실)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.23, no.2, 2010 , pp. 81-108 More about this Journal
Abstract
Objective : Thujae Orientalis Folium (TOF) can cool the blood and stop bleeding, eliminate phlegm and relieve cough in Oriental medicine. In addition, the fresh is used alone externally. Recently, TOF is known to have anti-tumor component. And also known to have tyrosinase inhibitory effect. Method : For these reasons, this study was designed to investigate anti-cancer and whitening activities of TOF. In this experiment, effects of TOF on proliferation rates of melanoma cells and on changes in genetic profiles were investigated. The genetic profile for the effect on human derived melanoma cell, SK-MEL-2, was measured using microarray technique, and the functional analysis on these genes was conducted. Results : Total 541 genes were up-regulated and 1,079 genes down-regulated in cells treated with TOF. Genes induced by TOF were mainly concerned with anti-cancer effects and apoptosis. Genes suppresed by TOF were related in extracellular signalling pathway. The network of total protein interactions was measured using cytoscape program, and some key molecules, such as THAP1, MAX1, STAM2, SMAD6, CYCS, PEX5, PSEN1, NONO, MAP2K7 and CREB1 that can be used for elucidation of therapeutical mechanism of medicine in future were identified. Conculusion : These results suggest possibility of TOF as anti-cancer drug for human melanoma. In addition, the present author also suggest that related mechanisms are involved in inhibition of several cancer pathway, activation of apoptosis pathway and suppression of general metabolic pathway.
Keywords
Thujae Orientalis Folium (TOF); anti-tumor component; tyrosinase inhibitory effect; anti-cancer; whitening activities; human melanoma;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Florell SR, Coffin CM, Holden JA, Zimmermann JW, Gerwels JW, Summers BK, Jones DA, Leachman SA.Preservation of RNA for functional genomic studies: a multidisciplinary tumor bank protocol.Mod Pathol. 2001 Feb;14(2):116-28.   DOI   ScienceOn
2 Liu X, Kim C, Yang J, Jemmerson R, Wang X (1996). "Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c". Cell 86(1):147–57.   DOI   ScienceOn
3 Shav-Tal Y, Zipori D. PSF and p54(nrb)/NonO--multi-functional nuclear proteins. FEBS Lett. 2002 Nov 6;531(2):109-14.   DOI   ScienceOn
4 Pavlícek A, Hrda S, Flegr J.Free-Tree--freeware program for construction of phylogenetic trees on the basis of distance data and bootstrap/jackknife analysis of the tree robustness. Application in the RAPD analysis of genus Frenkelia.Folia Biol (Praha). 1999;45(3):97-9.
5 Balch CM, Milton GW, Show HM, Cutaneous Melanoma : Clinical management and treatment result world-wide. Philadelphila, JB Lippincott. 1985:13-506.
6 Lee DH, Kim YC, Cho SH, Lee MG. Clinicopathologic analysis of malignant melanoma. Korean J Dermatol 2002;40:914-23.
7 Jang KA, Kim JH, Choi JH, Sung KJ, Moon KC, Koh JK. A clinico-histological study of malignant melanoma. Korean J Dermatol 2000;38:1435-43.
8 Martínez-Murillo F, Miller CL.Quality control for microarray analysis of human brain samples: The impact of postmortem factors, RNA characteristics, and histopathology.J Neurosci Methods. 2007 Sep 30;165(2):198-209. Epub 2007 Jun 7.   DOI   ScienceOn
9 Zahurak M, Parmigiani G, Yu W, Scharpf RB, Berman D, Schaeffer E, Shabbeer S, Cope L.Pre-processing Agilent microarray data. BMC Bioinformatics. 2007;8:142.   DOI
10 Grou CP, Carvalho AF, Pinto MP, Huybrechts SJ, Sa-Miranda C, Fransen M, Azevedo JE. Properties of the ubiquitin-pex5p thiol ester conjugate. J Biol Chem. 2009 Apr 17;284(16):10504-13.   DOI
11 Muniyappa H, Das KC. Activation of c-Jun N-terminal kinase (JNK) by widely used specific p38 MAPK inhibitors SB202190 and SB203580: a MLK-3-MKK7-dependent mechanism. Cell Signal. 2008 Apr;20(4):675-83.   DOI   ScienceOn
12 Chakraborty K, Maity PC, Sil AK, Takeda Y, Das S. cAMP stringently regulates human cathelicidin antimicrobial peptide expression in the mucosal epithelial cells by activating cAMP-response elementbinding protein, AP-1, and inducible cAMP early repressor. J Biol Chem. 2009 Aug 14;284(33):21810-27.   DOI
13 Kang, D E; Soriano S, Frosch M P, Collins T, Naruse S, Sisodia S S, Leibowitz G, Levine F, Koo E H (Jun. 1999). "Presenilin 1 facilitates the constitutive turnover of beta-catenin: differential activity of Alzheimer's disease-linked PS1 mutants in the beta-catenin-signaling pathway". J. Neurosci. (UNITED STATES) 19(11):4229-37.
14 汪認庵. 本草易讀. 北京; 人民衛生出版社. 1987:284-5.
15 천현자. Quercetin이 Melan-a 멜라닌 세포의 멜라닌 합성에 미치는 영향. 생약학회지. 2002;33(3):245-251.   과학기술학회마을
16 송화영. 백자인과 측백엽의 성분인 myricetin 이 멜라닌 생성에 미치는 영향. 익산: 원광대대학원. 2004.
17 Roslev P, King GM. Application of a Tetrazolium Salt with a Water-Soluble Formazan as an Indicator of Viability in Respiring Bacteria. Appl Environ Microbiol. 1993 Sep;59(9):2891-6.
18 Lazarow PB, Fujiki Y (1985). "Biogenesis of peroxisomes". Annu. Rev. Cell Biol. 1:489–530.   DOI   ScienceOn
19 辛民敎 외. 鄕藥集成方. 서울: 永林社. 1989:1039.
20 楊東喜. 本草備要解釋. 서울; 一中社. 1991: 309-10.
21 Lee MW, Koh JK, Kwon KS, Kim NI, Kim SW, Kim SN et al. Clinical and histopathological study of cutaneous melanoma in Korea. Korean J Dermatol 2003;41:43-7.
22 Rismanchi N, Puertollano R, Blackstone C. STAM adaptor proteins interact with COPII complexes and function in ER-to-Golgi trafficking. Traffic. 2009 Feb;10(2):201-17.   DOI   ScienceOn
23 Zhou Q, Heinke J, Vargas A, Winnik S, Krauss T, Bode C, Patterson C, Moser M. ERK signaling is a central regulator for BMP-4 dependent capillary sprouting. Cardiovasc Res. 2007 Dec 1;76(3):390-9.   DOI   ScienceOn
24 임종임. 측백엽 추출물의 항산화 효과. 서울大學校藥學論文集. 2001;26:7-14.
25 이수형. 측백엽 메탄올 추출물이 멜라닌 형성에 미치는 영향. 익산: 원광대 대학원. 2004.
26 탁민진. 측백엽 추출물의 발모효과에 관한 실험적 연구. 완주군: 우석대 대학원, 2006.
27 Roussigne, Myriam; Cayrol Corinne, Clouaire Thomas, Amalric Francois, Girard Jean-Philippe (Apr. 2003). "THAP1 is a nuclear proapoptotic factor that links prostate-apoptosis-response-4 (Par-4) to PML nuclear bodies". Oncogene (England) 22(16): 2432-42.   DOI   ScienceOn
28 Skulachev VP (1998). "Cytochrome c in the apoptotic and antioxidant cascades.". FEBS Lett. 423(3): 275–80.   DOI   ScienceOn
29 Wong, M W; Henry R W, Ma B, Kobayashi R, Klages N, Matthias P, Strubin M, Hernandez N (Jan. 1998). "The large subunit of basal transcription factor SNAPc is a Myb domain protein that interacts with Oct-1". Mol. Cell. Biol. (UNITED STATES) 18(1): 368-77.   DOI
30 Zhao Y, Burikhanov R, Qiu S, Lele SM, Jennings CD, Bondada S, Spear B, Rangnekar VM. 2007. Cancer resistance in transgenic mice expressing the SAC module of Par-4. Cancer Res. 67(19):9276-85.   DOI   ScienceOn
31 Chu, Z L; Pio F, Xie Z, Welsh K, Krajewska M, Krajewski S, Godzik A, Reed J C (Mar. 2001). "A novel enhancer of the Apaf1 apoptosome involved in cytochrome c-dependent caspase activation and apoptosis". J. Biol. Chem. (United States) 276(12): 9239-45.   DOI
32 申佶求. 申氏本草學. 서울: 壽文社. 1998:117, 175-6.
33 全國韓醫科大學本草學敎授共編. 本草學. 서울: 永林社. 1991:395, 494-5.
34 吳儀洛. 本草從新. 서울: 杏林書院. 1982:107-8.
35 Rottmann S, Speckgens S, Luscher-Firzlaff J, Luscher B. Inhibition of apoptosis by MAD1 is mediated by repression of the PTEN tumor suppressor gene. FASEB J. 2008 Apr;22(4):1124-34.
36 Hebenstreit D, Horejs-Hoeck J and Duschl A (2005) "JAK/STAT-dependent gene regulation by cytokines" in Drug News Perspect 18(4):243-9.   DOI   ScienceOn
37 Krainc, D; Bai G, Okamoto S, Carles M, Kusiak J W, Brent R N, Lipton S A (Oct. 1998). "Synergistic activation of the N-methyl-D-aspartate receptor subunit 1 promoter by myocyte enhancer factor 2C and Sp1". J. Biol. Chem. (UNITED STATES) 273(40): 26218-24.   DOI
38 Li, P; Nijhawan D, Budihardjo I, Srinivasula S M, Ahmad M, Alnemri E S, Wang X (Nov. 1997). "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade". Cell (UNITED STATES) 91(4): 479-89.   DOI   ScienceOn
39 Micheau, Olivier; Thome Margot, Schneider Pascal, Holler Nils, Tschopp Jurg, Nicholson Donald W, Briand Christophe, Grütter Markus G (Nov. 2002). "The long form of FLIP is an activator of caspase-8 at the Fas death-inducing signaling complex". J. Biol. Chem. (United States) 277(47): 45162-71.   DOI
40 Alberts B, Bray D, Hopin K, Johnson A, Lewis J, Raff M, Roberts K, Walter P (2004). "Tissues and Cancer". Essential cell biology. New York and London: Garland Science.
41 박대중, 이장천. 삼백초(三白草)에탄올추출물의 항산화능 및 피부 미백작용 연구. 대한본초학회지. 2008;23(2):193-202.   과학기술학회마을
42 Kimizuka F, Kato I. DNA chip technologies and their applications: towards functional genomics. Tanpakushitsu Kakusan Koso. 1998;43 (13):2004-11.
43 Magnus K. The nordic profile of skin cancer incidence. A comparative epidemiological study of the three main types of skin cancer. Int J Cancer 1991;47:12-9.   DOI
44 문구, 정벽학, 김병주, 암 동서의 결합치료. 원광대학교출판국:신이출판사. 1999:93-7.
45 Schroeder A, Mueller O, Stocker S, Salowsky R, Leiber M, Gassmann M, Lightfoot S, Menzel W, Granzow M, Ragg T. The RIN: an RNA integrity number for assigning integrity values to RNA measurements. BMC Mol Biol. 2006;7:3.   DOI   ScienceOn
46 Dunham I, Shimizu N, Roe BA, et al. (1999). "The DNA sequence of human chromosome 22.". Nature 402 (6761): 489–95.   DOI   ScienceOn
47 Shu, H B; Halpin D R, Goeddel D V (Jun. 1997). "Casper is a FADD- and caspase-related inducer of apoptosis". Immunity (UNITED STATES) 6(6): 751-63.   DOI   ScienceOn
48 Micheau, Olivier; Tschopp Jurg (Jul. 2003). "Induction of TNF receptor I-mediated apoptosis via two sequential signaling complexes". Cell (United States) 114(2):181-90.   DOI   ScienceOn
49 Sokol DL, Zhang X, Lu P, Gewirtz AM. Real time detection of DNA.RNA hybridization in living cells. Proc Natl Acad Sci USA. 1998;95(20):11538-43.   DOI   ScienceOn
50 김대수, 최정화, 김종한, 박수연, 강성인. 인동 등이 인간 유래 악성 흑색종 세포의 유전자 발현에 미치는 영향. 한방안이비인후피부과학회지. 2009;22(1):11-32.   과학기술학회마을
51 한진근, 박수연, 김종한, 최정화. 유백피 추출물이 인간의 피부 세포 재생 및 악성 흑색종 세포에 미치는 영향. 한방안이비인후피부과학회지. 2007;20(3):82-97.   과학기술학회마을
52 박용호, 김종한, 박수연, 최정화. DNA chip을 이용한 조각자 추출물의 인간 유래 악성 종양에 미치는 영향. 한방안이비인후피부과학회지. 2008;21(1):55-69.   과학기술학회마을
53 Cho KH, Cho MK, Lee YS, Ham EK. Clinical and histopathologic study of acral melanoma 1989;27:388-94.
54 Gillies Gg, Marks R, Foley P: Incidence. Incidence of nonmelanocytic skin cancer treated in Australia. Br Med J 1998;296:13.
55 Won YH, Hur SG, Lee SC, Chun IK, Kim YP. Clinical and histopathologic study of malignant melanoma (1980-1990.4). Korean J Dermatol 1991;29:193-202.