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Effects of atopic dermatitis induced materials on the expression of cytokine genes in human monocytes and mast cells  

Park, Koung-Suk (경원대학교 한의과대학 안이비인후피부과학 교실)
Kim, Kyung-Jun (경원대학교 한의과대학 안이비인후피부과학 교실)
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The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.23, no.2, 2010 , pp. 41-56 More about this Journal
Abstract
Objectives : On an experimental basis, the effects of atopic dermatitis induced materials on the expression of cytokine genes in human monocytes (THP-1, U937) and mast cells were studied. This study was carried out to be considered a fundamental knowledge in the research on the good of oriental medicine. Methods : After culturing THP-1, U937, and HMC-1, with the three different concentrations of LPS ($1\;{\mu}g/ml$), DPE ($10\;{\mu}g/ml$), and DNCB ($1\;{\mu}g/ml$), atopic dermatitis induced materials were treated in the culture medium. To investigate cytokine genes expression patterns, with lysis buffer and separation reagent, total RNA was extracted from THP-1, U937, and HMC-1 at intervals of 0, 12, 24, and 48 hours. Both cytokine mRNA expression patterns by atopic dermatitis induced materials and change of cytokine genes expression patterns in relation to atopy by selenium were analyzed with RT-PCR. Also IL-4 and INF-$\gamma$, which were secreted in the HMC-1, were analyzed using ELISA method. Results : 1. After treating THP-1 and U937 with LPS, DPE, and DNCB, there was no significant change in cytokine genes themselves, but various cytokines (IL-4, IL-6, IL-8, IL-13, IFN-$\gamma$, IFN-a, MCP-1, B2-MG) were expressed. 2. In the case of HMC-1, the expressions of IL-6 and IL-8 were significantly increased in the analysis of mRNA expression by dust mite allergens in DPE. 3. As a result of ELISA method, it is certain that IL-4 and IFN-$\gamma$ protein were secreted in the HMC-1 by DPE. 4. Selenium, an essential trace element, decreased the IL-10 and IL-13 expression in the HMC-1 by DPE. Conclusion : The results suggest that it is necessary to choose proper atopic dermatitis induced materials and suitable cultured cells in establishment of in vitro model of atopic dermatitis.
Keywords
RT-PCR; ELISA; mRNA; IL-6; IL-4; IFN-$\gamma$;
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1 Shirmali RK, Iron RD, Carlson BA, Sano Y, Gladyshev VN, Park JM, Hatfield DL. Selenoproteins mediate T cell immunity through an antioxidant mechanism. J Biol Chem 2008;283:20181-5.   DOI
2 Theoharides TC, Cochrane DE. Critical role of mast cells in inflammatory diseases and the effect of acute stress. J Neuroimmunol 2004;146:1-12.   DOI   ScienceOn
3 Shin TY, Kim SH, Kim DK, Leem KH,Park JS. Phlomis umbrosa root inhibits mast cell-dependent allergic reactions and inflammatory cytokine secretion. Phytotherapy Res 2008;22:153-8.   DOI   ScienceOn
4 Cetkovic-Cvrlje M and Eizirik DL. TNF and $IFN-\gamma$ potentiate the deleterious effects of $IL-1\beta$ on mouse pancreatic islets mainly via generation of nitric oxide. Cytokine. 1994;6:399-406.   DOI   ScienceOn
5 Chrousos GP. Stress, chronic inflammation, and emotional and physical well-being: Concurrent effects and chronic sequelae. J Allergy Clin Immunol 2000;106:S275-91.   DOI   ScienceOn
6 Koreck AI, Csoma Z, Bodai L, Ignacz F, Kenderessy AS, Kadocsa E, Szabo G, BorZ, Erdei A, Szony B, Homey B, Dobozy A, Kemedy L. Rhinophototherapy: A new therapeutic tool for themanagement of allergic rhinitis. J Allergy Clin Immunol 2005;115:541-7.   DOI   ScienceOn
7 Mc daniel ML, Kwon G, Hill JR, Marshall CA and Corbett JA. Cytokines and nitric oxides in islet inflammation and diabetes. Proc. Soc. Exp. Biol. Med 1996;211:24-32.   DOI
8 Williams CM, Galli SJ. The diverse potential effector and immunoregulatory roles of mast cells in allergic disease. J Allergy Clin Immunol 2000;105:847–59.   DOI   ScienceOn
9 김정원. 알레르기 및 면역학적 관점에서의 아토피피부염. 대한피부과학회지. 2003;41(6):687-9.
10 안성구. COMMON SKIN DISEASE. 서울: 퍼시픽출판사. 2003:68.
11 Hataguchi Y, Tai H, Nakajima H, Kimata H. Drinking deep-sea water restores mineral imbalance in atopic eczema/dermatitis syndrome. Eur J Clin Nutr 2005;59:1093-6.   DOI   ScienceOn
12 Obara W, Kawa Y, Ra C, Nishioka K, Soma Y, Mizoguchi M. T cells and mast cells as a major source of interleukin-13 in atopic dermatitis. Dermatology 2002;205:11-7.   DOI   ScienceOn
13 Lee JS, Kim IS, Kim JH, Kim JS, Kim DH, Yun CY. Suppressive effects of Houttuynia cordata Thunb (Saururaceae) extract on Th2 immune response. J Ethnopharmacol 2008;117(1):34-40.   DOI   ScienceOn
14 Bisset LR, Schmid-Grendelmeier P. Chemokines and their receptors in the pathogenesis of allergic asthma: progress and perspective. Curr Opin Pulmon Med 2005;11:35–42.   DOI   ScienceOn
15 Na HJ, Shin HY, Kim NH, Kwon MW, Park EJ, Hong SH, Kim NI, Kim HM. Regulatory effects of cytokine production in atopic allergic reaction by Gammidanguieumja. Inflammation 2004;28:291-8.   DOI   ScienceOn
16 Kim IS, Kim JH, Kim JS, Yun CY, Kim DH, Lee JS. The inhibitory effect of Houttuynia cordata extract on stem cell factor-induced HMC-1 cell migration. J Ethnopharmacol 2007;112(1):90-5.   DOI   ScienceOn
17 김정희. 아토피피부염의 최신 지견. 소아알레르기 및 호흡기학회지. 2004;14(1):12-23.
18 Homey B, Steinhoff M, Ruzicka T, Leung DY. Cytokines and chemokines orchestrate atopic skin inflammation. J Allergy Clin Immunol 2006;118:178–89.   DOI   ScienceOn
19 Arlian LG, Platts-Mills TA. The biology of dust mites and the remediation of mite allergens in allergic disease. J Allergy Clin Immunol 2001;107:S406–S413.   DOI   ScienceOn
20 Bellanti JA. Cytokines and allergic diseases: clinical aspects. Allergy Asthma Proc 1998;19:337-41.   DOI   ScienceOn
21 Qgawa H, Yoshiike T. A speculative view of atopic dermatitis: barrier dysfunction in pathogenesis. J Dermatol Sci. 1993;5:197-204.   DOI   ScienceOn
22 Paul WE. Interleukin-4: a prototypic immunoregulatory lymphokine. Blood 1991;77:1859–70.
23 나영호. 아토피피부염 발병기전에서 케모카인의 역할. 소아알레르기 및 호흡기학회지. 2005;15(3):238-41.
24 Heijink IH, Van Oosterhout AJ. Targeting T cells for asthma. Curr Opin Pharmacol 2005;5:227–31.   DOI   ScienceOn
25 Heinle K, Adam A, Gradl M, Wiseman M, Adam O. Selenium concentration in erythrocytes of patients with rheumatoid arthritis. Clinical and laboratory chemistry infection markers during administration of selenium. Med Klin 1997;Suppl 3:29-3.
26 Lee JS, Kim IS, Ryu JS, Yun CY. House dust mite, Dermatophagoides pteronissinus increases expression of MCP-1, IL-6, and IL-8 in human monocytic THP-1 cells. Cytokine. 2008;42:365-71.   DOI   ScienceOn
27 Kim DB, Kim JH, Kwon SH, Kim YJ, Lee SH, Lee YH, Seo JN, Park CS, Park KL, Kwon HJ. Regulation of macrophage inflammatory protein-2 gene expression in response to 2,4-dinitrofluorobenzene in RAW264.7 cells. 2008;41(4):316-21.
28 대한피부과학회 교과서 편찬위원회. 개정4판 피부과학. 서울:여문각. 2001:161-6.
29 이명희. 셀레늄 영양과 건강. 대한암예방학회지 2003;8:36-44.
30 Perez A, Neve J, Desmedt J, Duchateau J, Dramaix M, Famaey JP. Lymphocytes response is enhanced by supplementation of elderly subjects with selenium enriched yeast. Am J Clin Nutr 1991;53;1323-8.
31 Kiremidjian-Schmacher L, Roy M, Wishe HL, Cohen MW, Stotzky G. Supplementation with selenium and human immune cell functions. Biol Trace Elem Res 1994;41:115-27.   DOI
32 Borich L. Allergic rhinitis: Systemic inflammation and implications for management. J Allergy Clin Immunol. 2002:60-2.
33 Kiremidjian-Schmacher L, Roy M, Wishe HL, Cohen MW, Stotzky G. Supplementation with selenium arguments the functions of natural killer and lymphokine activated killer cells. Biol Trace Elem Res 1996;52:227-39.   DOI   ScienceOn
34 Friedmann PS, Tan BB, Musaba E, Pathogenesis and management of atopic dermatitis. Clin. Exp. Allergy. 1995;25:799-806.   DOI   ScienceOn
35 Brown KM, Arthur JR. Selenium, Selenoproteins, and human health: a review. Public Health Nutrition 2001;4:593-9.
36 Navaro-alarcon N, Lopez-Martinez MC. Essentiality of selenium in the human body: relationship with different disease. Sci Total Environ 2000;249:347-71.   DOI   ScienceOn
37 박용민. 아토피피부염 병태생리에 대한 최신지견. 소아알레르기 및 호흡기. 2006;16(3):189-96.
38 Corbett JA, and Mac Daniel ML. Intraislet release of interleukin-1 inhibits beta cell function by inducing beta cell expression of inducible nitric oxide syntheses. J Exp Med. 1995;181:559-68.   DOI   ScienceOn
39 Yang SH, Hong CY, Yu CL. Decreased serum IgE level, decreased $INF-\gamma$ and IL-5 but increased IL-10 production, and suppresed cyclooxygenase 2 mRNA expression in patients with perennial allergic rhinitis after treatment with a new mixed formula of Chinese herbs. Int Immunopharmacol. 2001;1(6):1173-82.   DOI   ScienceOn
40 Higuchi M, Higashi N, Taki H, Osawa T. Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L-arginine dependent mechanism acts as synergistically as the major cytolytic mechanism of activated macrophages. J Immunol. 1990;144:1425-31.