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Anti-inflammaory effects of the Gamroeum in vivo and in vitro  

Lim, In-Ho (대구한의대학교 안이비인후피부과교실)
Jung, Ho-Jun (대구한의대학교 안이비인후피부과교실)
Kim, Sang-Chan (대구한의대학교 한의과대학)
Jee, Seon-Young (대구한의대학교 안이비인후피부과교실)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.23, no.2, 2010 , pp. 13-26 More about this Journal
Abstract
Objectives : The present study was conducted to evaluate the anti-inflammatory effects of the Gamroeum water extracts (GRE) in vivo and in vitro. Methods : The effects of GRE on anti-inflammation were measured by production of NO, $PGE_2$ (Prostaglandin $E_2$), iNOS (inducible Nitric Oxide Synthase), COX-2, $NF{\kappa}B$ (Nuclear Factor kappa B), TNF-$\alpha$ (Tumor Necrosis Factor-alpha) and IL-$1{\beta}$ (Interleukin-$1{\beta}$), IL-6 in Raw 264.7 macrophage cells stimulated with LPS. Results : 1. In machrophage cells, LPS displayed significant stimulatory effects on the production of NO and $PGE_2$. However, GRE showed significant inhibitory effects on NO and $PGE_2$ release. The level of NO and $PGE_2$ was decreased by GRE in a concentration dependent manner as compared with LPS only group. 2. Immunoblot analysis verified that LPS stimulation significantly increased the iNOS and COX-2 protein level, but GRE suppressed the induction of iNOS and COX-2 protein at a concentration dependent manner. 3. GRE reduced the elevated production of TNF-$\alpha$, IL-$1{\beta}$ and IL-6 by LPS. Moreover, the inhibitory effects of GRE was occurred in a dose-dependent manner. 4. GRE significantly reduced the expression of NF-${\kappa}B$ protein in nuclear fraction. 5. GRE effectively inhibited the increases of hind paw skin thicknesses and inflammatory cell infiltrations induced by carrageenan treatment. It, therefore, considered that GRE will be favorably inhibited the acute edematous inflammations. Conclusions : These results indicated that GRE could have anti-inflammatory capacity by inhibiting the production of NO, $PGE_2$ and cytokines in vitro and by reducing the formation of carrageenan-induced paw edema in vivo. Moreover, inhibitory effects of GRE on the macrophage activation were attributable to the reduction of some of inflammatory factors by inhibiting iNOS and COX-2 through the suppression of NF-${\kappa}B$.
Keywords
Gamroeum; Anti-inflammaory effects; NO; $PGE_2$; iNOS; COX-2; $NF{\kappa}B$; TNF-$\alpha$; IL-$1{\beta}$; IL-6;
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Times Cited By KSCI : 9  (Citation Analysis)
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1 Hseu YC, Wu FY, Wu JJ, Chen JY, Chang WH, Lu FJ, Lai YC, Yang HL. Anti-inflammatory potential of Antrodia Camphorata through inhibition of iNOS, COX-2 and cytokines via the NF-kappaB pathway. Int Immunopharmacol. 2005; 5:1914-25.   DOI   ScienceOn
2 Lee YS, Kim HS, Kim SK, Kim SD. IL-6 mRNA expression in mouse peritoneal macrophages and NIH3T3 fibroblasts in response to Candida albicans. J Microbiol Biotechnol. 2000;10(1):8-15.
3 Lee BG, Kim SH, Zee OP, Lee KR, Lee HY, Han JW, Lee HW. Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophages by two-carboline alkaloids extracted from Melia azedarach. Eur J Pharmacol. 2000;406(3):301-9.   DOI   ScienceOn
4 Kubes P. Inducible nitric oxide synthase: a little bit of good in all of us. Gut. 2000;47(1):6-9.   DOI   ScienceOn
5 McDaniel ML, Kwon G, Hill JR, Marshall CA, Corbett JA. Cytokines and nitric oxides in islet inflammation and diabetes. Proc Soc Exp Biol Med. 1996;211(1):24-32.   DOI
6 Delgado AV, McManus AT, Chambers JP. Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance P. Neuropeptides. 2003;37(6):355-61.   DOI   ScienceOn
7 Chiou WF, Chou CJ, Chen CF. Camptothecin suppresses nitric oxide biosynthesis in RAW 264.7 macrophages. Life Sci. 2001;69(6):625-35.   DOI   ScienceOn
8 Seo WG, Pae HO, Oh GS, Kim NY, Kwon TO, Shin MK, Chai KY, Chung HT. The aqueous extract of Rhodiola sachalinensis root enhances the expression of inducible nitric oxide synthase gene in RAW 264.7 macrophages. J Ethnopharmacol. 2001;76(1):119-23.   DOI   ScienceOn
9 Higuchi M, Higashi N, Taki H, Osawa T. Cytolytic mechanisms of activated macrophages. Tumor necrosis factor and L-arginine-dependent mechanisms act synergistically as the major cytolyticmechanisms of activated macrophages. J Immunol. 1990;144(4):1425-31.
10 염정호, 오경재, 유영천. 니켈 및 코발트의 세포독성 기전에서 Nitric Oxide의 역할. 대한산업의학회지. 2001;13(3):274-85.
11 전국한의과대학 본초학교수. 본초학. 서울:영림사. 1995:178-9, 191, 329, 352, 476, 541, 580, 589-90, 592.
12 이재용, 황치원. 齒衄의 治療에 對한 文獻的考察. 대전대학교 한의학연구소 논문집. 2000;8(2):191-210.
13 임성우. 수종 한약 처방 전탕액의 허피즈바이러스에 대한 효능 검색. 한방내과학회지 2000;21(2):291-7.
14 Kim HD, Cho HR, Moon SB, Shin HD, Yang KJ, Park BR, Jang HJ, Lim LS, Lee HS, Ku SK. Effect of exopolymers from Aureobasidum pullulans on formalininduced chronic paw inflammation in mice. J Microbiol Biotechnol. 2006;16(12):1954-60.   과학기술학회마을
15 Wink DA, Mitchell JB. Chemical biology of nitric oxide: Insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide. Free Radic Biol Med. 1998;25:434-56.   DOI   ScienceOn
16 서은성, 황충연, 김남권. 加味淸上防風湯의 항염증 효과에 대한 실험적 연구. 한방안이비인후피부과학회지. 2008;21(2):54-70.   과학기술학회마을
17 박혜준, 윤화정, 윤정원, 윤소원, 고우신. 五味消毒飮이 마우스 복강 대식세포에서 NO의 분비과정에 미치는 영향. 동의생리병리학회지. 2002;16(5):921-7.   과학기술학회마을
18 이상문, 홍승욱. Mouse cell에서 托裏消毒飮의 항산화작용과 항염증효과. 한방안이비인후피부과학회지. 2007;20(3):43-50.   과학기술학회마을
19 Lim JH, Lee JR, Kim SC, Jee SY. Inhibitory effect of Yongdamsagantang water extract on IL-6 and nitric oxide production in lipopolysaccharide-activated RAW 264.7 cells. Oriental Pharmacy and Experimental Medicine. 2007;7(3):321-9.   과학기술학회마을   DOI
20 한의학대사전 편찬위원회. 한의학대사전. 서울: 정담. 1998:44.
21 Chun SC, Jee SY, Lee SG, Park SJ, Lee JR, Kim SC. Anti-Inflammatory activity of the methanol extract of Moutan Cortex in LPS-activated RAW 264.7 cells. Evid Based Complement Alternat Med. 2007;4(3): 327-33.   DOI   ScienceOn
22 Yoon TG, Byun BH, Kwon TK, Suh SI, Byun SH, Kwon YK, Kim SC. Inhibitory effect of Farfarae Flos water extract on COX-2, iNOS expression and nitric oxide production in lipopolysaccharide-activated RAW 264.7 cells. Korean J oriental Physiology & Pathology. 2004;18(3):908-13.   과학기술학회마을
23 이영선, 한옥경, 신상우, 박종현, 권영규. 향부자 열수추출물의 Nitric oxide 생성 및 iNOS 유전자 발현에 미치는 영향. 동의생리병리학회지. 2003;17(3):771-6.   과학기술학회마을
24 Seo WG, Pae HO, Oh GS, Chai KY, Kwon TO, Yun YG, Kim NY, Chung HT. Inhibitory effects of methanol extract of Cyperus rotundus rhizomes on nitric oxide and superoxide production by murine macrophage cell line, RAW 264.7 cells. J Ethnopharmacol. 2001;76(1):59-64.   DOI   ScienceOn
25 장선일, 김형진, 황기명, 배현옥, 윤용갑, 정헌택, 김윤철. 활성화된 설치류 RAW 264.7 대식세포에서 당귀 에탄올추출물의 항염증 효과. 대한한의학방제학회지. 2002;10(2):189-97.   과학기술학회마을
26 Lee JH, Choi YH, Choi BT. The anti-inflammatory effects of 2 Hz electroacupuncture with different intensities on acute carrageenan-induced inflammation in the rat paw. Int J Mol Med. 2005;16(1):99-102.
27 Kawamata H, Ochiai H, Mantani N, Terasawa K. Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS-activated RAW 264.7 cells, a murine macrophage cell line. Am J Chin Med. 2000;28(2):217-26.   DOI   ScienceOn
28 太平惠民和劑局. 太平惠民和劑局方. 北京:人民衛生出版社. 1985:216.
29 Shen Q, Chen F, Luo J. Comparison studies on chemical constituents of essential oil from Ramulus Cinnamomi and Cortex Cinnamomi by GC-MS. Zhong Yao Cai. 2002;25(4):257-8.
30 Fox JB, Doerr RC, Lakritz L. Interaction between sample preparation techniques and three methods of nitrite determination. J Assoc Off Anal Chem. 1982;65(3):690-5.
31 Cetkovic-Cvrlje M, Eizirik DL. $TNF-\alpha$ and $IFN-\gamma$ potentiate the deleterious effects of $IL-1\beta$ on mouse pancreatic islets mainly via generation of nitric oxide. Cytokine. 1994;6(4):399-406.   DOI   ScienceOn
32 Gupta M, Mazumder UK, Gomathi P, Selvan VT. Antiinflammatory evaluation of leaves of Plumeria acuminata. BMC Complement Altern Med. 2006;6:36.   DOI
33 Holt S, Comelli F, Costa B, Fowler CJ. Inhibitors of fatty acid amide hydrolase reduce carrageenan-induced hind paw inflammation in pentobarbital-treated mice: comparison with indomethacin and possible involvement of cannabinoid receptors. Br J Pharmacol. 2005;146 (3):467-76.   DOI   ScienceOn
34 Sarkar D, Saha P, Gamre S, Bhattacharjee S, Hariharan C, Ganguly S, Sen R, Mandal G, Chattopadhyay S, Majumdar S, Chatterjee M. Anti-inflammatory effect of allylpyrocatechol in LPS-induced macrophages is mediated by suppression of iNOS and COX-2 via the $NF-\kappaB$ pathway. Int Immunopharmacol. 2008;8(9):1264-71.   DOI   ScienceOn
35 Wang MT, Honn KV, Nie D. Cycloxygenases, prostanoids and tumor progression. Cancer Metastasis Rev. 2007;26:525-34.   DOI   ScienceOn
36 Chen F, Castranova V, Shi X. New insights into the role of nuclear factor-kappa B in cell growth regulation. Am J Pathol. 2001;159(2):387-97.   DOI   ScienceOn
37 Palmer RM, Ashton DS, Moncada S. Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature. 1998; 333:664-6.
38 Lee AK, Sung SH, Kim YC, Kim SG. Inhibition of lipopolysaccharide-inducible nitric oxide synthase, $TNF-\alpha$ and COX-2 expression by sauchinone effects on I-$\kappa$B $\alpha$ phosphorylation, C/EBP and AP-1 activation. British journal of pharmacology. 2003;139(1):11-20.   DOI   ScienceOn
39 신경민, 박영미, 김인태, 홍선표, 홍정표, 이경태. Amygdalin의 Murine Macrophage RAW 264.7 세포에서 in vitro 항염효과. 생약학회지. 2003;34(3):223-7.   과학기술학회마을
40 Feldmann M, Brennan FM, Maini RN. Role of cytokines in rheumatoid arthritis. Annu Rev Immunol. 1996;14:397-440.   DOI   ScienceOn