Browse > Article
http://dx.doi.org/10.12701/yujm.2018.35.1.63

Reliability and validity of free software for the analysis of locomotor activity in mice  

Hong, Yoo Rha (Department of Pediatrics, College of Medicine, Kosin University)
Moon, Eunsoo (Department of Psychiatry, Pusan National University Hospital)
Publication Information
Journal of Yeungnam Medical Science / v.35, no.1, 2018 , pp. 63-69 More about this Journal
Abstract
Background: Kinovea software that tracking semi-automatically the motion in video screen has been used to study motion-related tasks in several studies. However, the validation of this software in open field test to assess locomotor activity have not been studied yet. Therefore, this study aimed to examine the reliability and validity of this software in analyzing locomotor activities. Methods: Thirty male Institute Cancer Research mice were subjected in this study. The results examined by this software and the classical method were compared. Test-retest reliability and inter-rater reliability were analyzed with Pearson's correlation coefficient and intraclass correlation coefficient (ICC). The validity of this software was analyzed with Pearson's correlation coefficient. Results: This software showed good test-retest reliability (ICC=0.997, 95% confidence interval [CI]=0.975-0.994, p<0.001). This software also showed good inter-rater reliability (ICC=0.987, 95% CI=0.973-0.994, p<0.001). Furthermore, in three analyses for the validity of this software, there were significant correlations between two methods (Pearson's correlation coefficient=0.928-0.972, p<0.001). In addition, this software showed good reliability and validity in the analysis locomotor activity according to time interval. Conclusion: This study showed that this software in analyzing drug-induced locomotor activity has good reliability and validity. This software can be effectively used in animal study using the analysis of locomotor activity.
Keywords
Locomotor activity; Video-tracking software; Reliability; Validity; Open field test;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Tosini G. Locomotor activity in rodents. Methods Mol Biol 2007;362:95-101.
2 Gray VC, Hughes RN. Drug-, dose- and sex-dependent effects of chronic fluoxetine, reboxetine and venlafaxine on openfield behavior and spatial memory in rats. Behav Brain Res 2015;281:43-54.   DOI
3 Roybal K, Theobold D, Graham A, DiNieri JA, Russo SJ, Krishnan V, et al. Mania-like behavior induced by disruption of CLOCK. Proc Natl Acad Sci U S A 2007;104:6406-11.   DOI
4 Machado DG, Cunha MP, Neis VB, Balen GO, Colla A, Grando J, et al. Fluoxetine reverses depressive-like behaviors and increases hippocampal acetylcholinesterase activity induced by olfactory bulbectomy. Pharmacol Biochem Behav 2012;103:220-9.   DOI
5 Machado DG, Bettio LE, Cunha MP, Capra JC, Dalmarco JB, Pizzolatti MG, et al. Antidepressant-like effect of the extract of Rosmarinus officinalis in mice: involvement of the monoaminergic system. Prog Neuropsychopharmacol Biol Psychiatry 2009;33:642-50.   DOI
6 Tzschentke TM. Measuring reward with the conditioned place preference (CPP) paradigm: update of the last decade. Addict Biol 2007;12:227-462.   DOI
7 Thiel KJ, Okun AC, Neisewander JL. Social reward-conditioned place preference: a model revealing an interaction between cocaine and social context rewards in rats. Drug Alcohol Depend 2008;96:202-12.   DOI
8 Bondar NP, Lepeshko AA, Reshetnikov VV. Effects of early-life stress on social and anxiety-like behaviors in adult mice: sex-specific effects. Behav Neurol 2018;2018:1538931.
9 Niikura K, Ho A, Kreek MJ, Zhang Y. Oxycodone-induced conditioned place preference and sensitization of locomotor activity in adolescent and adult mice. Pharmacol Biochem Behav 2013;110:112-6.   DOI
10 Walsh RN, Cummins RA. The open-field test: a critical review. Psychol Bull 1976;83:482-504.   DOI
11 Jung SH, Park JM, Moon E, Chung YI, Lee BD, Lee YM, et al. Delay in the recovery of normal sleep-wake cycle after disruption of the light-dark cycle in mice: a bipolar disorderprone animal model? Psychiatry Investig 2014;11:487-91.   DOI
12 Zhang JJ, Kong Q. Locomotor activity: a distinctive index in morphine self-administration in rats. PLoS One 2017;12:e0174272.   DOI
13 Moral-Munoz JA, Esteban-Moreno B, Arroyo-Morales M, Cobo MJ, Herrera-Viedma E. agreement between face-to-face and free software video analysis for assessing hamstring flexibility in adolescents. J Strength Cond Res 2015;29:2661-5.   DOI
14 Otero L, Zurita M, Aguayo C, Bonilla C, Rodriguez A, Vaquero J. Video-tracking-box linked to smart software as a tool for evaluation of locomotor activity and orientation in brain-injured rats. J Neurosci Methods 2010;188:53-7.   DOI
15 Kulikov AV, Tikhonova MA, Kulikov VA. Automated measurement of spatial preference in the open field test with transmitted lighting. J Neurosci Methods 2008;170:345-51.   DOI
16 Guzman-Valdivia CH, Blanco-Ortega A, Oliver-Salazar MA, Carrera-Escobedo JL. Therapeutic motion analysis of lower limbs using Kinovea. Int J Soft Comput Eng 2013;3:359-65.
17 Balsalobre-Fernandez C, Tejero-Gonzalez CM, del Campo-Vecino J, Bavaresco N. The concurrent validity and reliability of a low-cost, high-speed camera-based method for measuring the flight time of vertical jumps. J Strength Cond Res 2014;28:528-33.   DOI
18 Baude M, Hutin E, Gracies JM. A bidimensional system of facial movement analysis conception and reliability in adults. Biomed Res Int 2015;2015:812961.
19 Padulo J, Vando S, Chamari K, Chaouachi A, Bagno D, Pizzolato F. Validity of the MarkWiiR for kinematic analysis during walking and running gaits. Biol Sport 2015;32:53-8.
20 Elwardany SH, El-Sayed WH, Ali MF. Reliability of Kinovea computer program in measuring cervical range of motion in sagittal plane. Open Access Libr J 2015;2:e1916.
21 Abd Elrahim RM, Embaby EM, Ali MF, Kamel RM. Interrater and intra-rater reliability of Kinovea software for measurement of shoulder range of motion. Bull Fac Phys Ther 2016;21:80-7.   DOI
22 Grigg J, Haakonssen E, Rathbone E, Orr R, Keogh JWL. The validity and intra-tester reliability of markerless motion capture to analyse kinematics of the BMX supercross gate start. Sports Biomech 2017:1-19.
23 Fleiss JL. The design and analysis of clinical experiments. New York: Wiley; 1986.
24 Koo TK, Li MY. A guideline of selecting and reporting intraclass correlation coefficients for reliability research. J Chiropr Med 2016;15:155-63.   DOI
25 Mukaka MM. Statistics corner: a guide to appropriate use of correlation coefficient in medical research. Malawi Med J 2012;24:69-71.