Browse > Article

Imaging Studies in Mouse Brain Using Clinical 3T MRI Scanner  

Lim, Soo-Mee (Department of Radiology, Ewha University Mokdong Hospital, School of Medicine, Ewha Womans University)
Park, Eun-Mi (Department of Pharmacology, Ewha University Mokdong Hospital, School of Medicine, Ewha Womans University)
Publication Information
Progress in Medical Physics / v.21, no.4, 2010 , pp. 348-353 More about this Journal
Abstract
The purpose of this study was to explore the potentials of a clinical 3T MRI in mouse brains and technical adaptation and optimization. T1-weighted images (T1WI), T2-weighted images (T2WI), FLAIR (Fluid Attenuated Inversion Recovery) images, Gadolinium enhanced T1-weighted images (Gd-T1WI), Diffusion weighted images (DWI) were acquired in brain of 2 mice (weight 20~25 g) with cerebral infarction by occlusion of right middle cerebral artery, 1 hour, 24 hours, 72 hours after infarction and 1 normal mouse brain using clinical 3T MRI scanner. We analyzed differentiation of striatum, ventricle, cerebral cortex, and possibility of detection of acute cerebral infarction. We could differentiate the striatum, ventricle, cerebral cortex on T2WI and on DWI, FLAIR, T1WI, the differentiation of each anatomy of brain was not definite, but acute cerebral infarction was detected on DWI of 1 hour, 24 hours, 72 hours after infarction and on T2WI, FLAIR of 24 hours, 72 hours after infarction. Clinical 3T MRI can be used in differentiation of anatomy of mouse brains and DWI can be helpul in detection of acute cerebral infarction in acute phase. With technical adaptation and optimization clinical 3T MRI can be useful tool for provide preclinical and clinical small animal studies.
Keywords
Magnetic resonance imaging (MRI); Small animal; Brain; Imaging diagnosis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Clavien PA, Selzner M, Rudiger HA, et al: A prospective randomized study in 100 consecutive patients undergoing major liver resection with versus without ischemic preconditioning. Ann Surg 238:843-850 (2003)   DOI   ScienceOn
2 Huisman TA: Diffusion-weighted imaging: basic concepts and application in cerebral stroke and head trauma. Eur Radiol 13:2283-2297 (2003)   DOI   ScienceOn
3 Chen F, Keyzer FD, Wang H, et al: Diffusion weighted imaging in small rodents using clinical MRI scanners. Methods 43:12-20 (2007)   DOI   ScienceOn
4 Bammer R: Principles of diffusion weighted image. Eur J Radiol 45:169-184 (2003)   DOI   ScienceOn
5 Thoeny HC, Keyzer FD, Chen F, et al: Diffusion-weighted MR imaging in monitoring the effect of a vascular targeting agent on rhabdomyosarcoma in rats. Radiology 234:756-764 (2005)   DOI   ScienceOn
6 Eida S, Sumi M, Sakihama N, Takahashi H, Nakamura T: Apparent diffusion coefficient maping of salivary tumors: Prediction of benignancy and malignancy. Am J Neuroradiol 28: 116-121 (2007)
7 Wegener S, Gottschalk B, Jovanovic V, et al: Transient ischemic attacks before ischemic stroke: preconditioning the human brain? A multicenter magnetic resonance imaging study. Stroke 35:616-621 (2004)   DOI   ScienceOn
8 Bihan DL, Poupon A, Amadon A, Lethimonnier F: Artifacts and fitfalls in diffusion MRI. J Magn Reson Imaging 24:478-488 (2006)   DOI   ScienceOn
9 Thoeny HC, Keyzer FD: Extracranial applications of diffusion- weighted magnetic resonance image. Eur Radiol 17:1385-1393 (2007)   DOI   ScienceOn
10 Hayashida Y, Hiral T, Morishita S, et al: Diffusion-weighted image in metastatic brain tumors: comparison of histologic type and tumor cellularity. Am J Neuroradiol 27:1419-1425 (2006)
11 Rumboldt Z, Camacho DLA, Lake D, Welsh CT, Castillo M: Apparent diffusion coefficient for differentiation of cerebellar tumors in children: Am J Neuroradiol 27:1362-1369 (2006)
12 Pasupathy S, Homer-Vanniasinkam S: Ischemic preconditioning protects against ischaemia/reperfusion injury: emerging concepts. Eur J Vasc Endovasc Surg 29:106-115 (2005)   DOI   ScienceOn
13 Part EM, Cho S, Frys K, et al: Interaction between inducible nitric oxide synthase and poly(ADP-ribose) polymerase in focal ischemic brain injury. Stroke 35:2896-2901 (2004)   DOI   ScienceOn
14 Park JW, Kim HJ, Song GS, Han HS: Blood-brain barrier experiments with clinical magnetic resomance imaging and and immunohistochemical study. J Korean Neurosurg Soc 47:203-209 (2010)   과학기술학회마을   DOI
15 Christian TF, Gonyea J, Bell SP, Andrews T: Mouse cardiac MRI on a 3T clinical system using a low cost setup. J of Cardiovascular Magnetic Resomance 11:253 (2009)   DOI
16 Weih M, Bergk A, Isaev NK, et al: Attenuated stroke severity after prodromal TIA: a role for ischemic tolerance in the brain. Stroke 30:1851-1854 (1999)   DOI   ScienceOn
17 Yellon DM, Alkhulaifi AM, Pugsley WB: Preconditioning the human myocardium. Lancet 342:276-277 (1993)   DOI   ScienceOn