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http://dx.doi.org/10.5808/GI.2011.9.4.161

Gene Expression Analysis of Gα13-/- Knockout Mouse Embryos Reveals Perturbations in Gα13 Signaling Related to Angiogenesis and Hypoxia  

Park, Ji-Hwan (Department of Molecular Science and Technology, Ajou University)
Choi, Sang-Dun (Department of Molecular Science and Technology, Ajou University)
Publication Information
Genomics & Informatics / v.9, no.4, 2011 , pp. 161-172 More about this Journal
Abstract
Angiogenesis is regulated by a large number of molecules and complex signaling mechanisms. The G protein $G{\alpha}_{13}$ is a part of this signaling mechanism as an endothelial cell movement regulator. Gene expression analysis of $G{\alpha}_{13}$ knockout mouse embryos was carried out to identify the role of $G{\alpha}_{13}$ in angiogenesis signaling during embryonic development. Hypoxia-inducible response factors including those acting as regulators of angiogenesis were over expressed, while genes related to the cell cycle, DNA replication, protein modification and cell-cell dissociation were under expressed. Functional annotation and network analysis indicate that $G{\alpha}_{13}{^{-/-}}$ embryonic mice were exposed to hypoxic conditions. The present analysis of the time course highlighted the significantly high levels of disorder in the development of the cardiovascular system. The data suggested that hypoxia-inducible factors including those associated with angiogenesis and abnormalities related to endothelial cell division contributed to the developmental failure of $G{\alpha}_{13}$ knockout mouse embryos.
Keywords
angiogenesis; gene expression; G protein $G{\alpha}_{13}$; G protein signaling; hypoxia;
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