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Identification of Novel Genes with Proapoptotic Activity  

Kang Eun-Ju (Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kim Jeong-Min (Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kim Na-Young (Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Park Kyung-Mi (Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Park Seong-Min (Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kim Nam-Soon (Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Yoo Hyang-Sook (Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Yeom Young-Il (Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kim Soo-Jung (Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Genomics & Informatics / v.4, no.2, 2006 , pp. 77-79 More about this Journal
Abstract
In order to identify novel proapoptotic genes, we screened approximately 1,000 hypothetical genes whose functions are completely unknown. After these genes were transiently expressed in HeLa cells, their nuclei images were captured using automated high-speed fluorescence microscope, through which the ratio of apoptotic nuclei was estimated. We selected genes that induce greater than 3-fold increase in apoptotic nuclei compared to that of the vector control. The candidate proapoptotic genes were sequenced and their effects on cell death were further confirmed by the additional assay, DNA fragmentation ELISA. Finally, we were able to identify 4 full-length hypo-thetical genes with proapoptotic activity.
Keywords
Novel proapoptotic genes; apoptosis; fluorescence microscope; apoptotic nuclei;
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