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http://dx.doi.org/10.15207/JKCS.2020.11.5.061

Convergence Study on Preparation of Anti-aging Peptides from Fish Collagen Hydrolysates  

Bae, In Young (Food and Nutrition, Far East University)
Han, Yoo Kyung (Food and Nutrition, Hanyang University)
Je, Hyun Jeong (Food and Nutrition, Hanyang University)
Lee, Hyun Jun (Nong Shim Co., Ltd.)
Lee, Hyeon Gyu (Food and Nutrition, Hanyang University)
Publication Information
Journal of the Korea Convergence Society / v.11, no.5, 2020 , pp. 61-72 More about this Journal
Abstract
An anti-aging peptide was prepared from fish collagen hydrolysate (FCH) by ultrafiltration (MWCO; 1 kDa) and reversed-phased high performance liquid chromatography (RP-HPLC). Its anti-aging properties were evaluated based on the procollagen-synthesizing and MMP-1-inhibiting activities in Hs68 cells. A potent anti-aging peptide (fraction I-I) increased collagen synthesis by 46% and also inhibited MMP-1 secretion by 77%, compared with unpurified FCH. The amino acid sequence of fraction I-I was identified to be Gly-Arg-Arg-Gly-Asn-Lys (GRRGNK; the repeating Gly-X-Y sequence in collagen), and it had a molecular mass of 686.175 Da. It revealed that the anti-aging activity of GRRGNK was mainly due to skin protective effects. These results demonstrated that fish collagen hydrolysate may be a potential source of anti-aging peptides, which could be utilized in various field, including foods, cosmetics, and pharmaceuticals.
Keywords
Fish collagen hydrolysate; Anti-aging peptide; Collagen synthesis; MMP-1 inhibition; GRRGNK;
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Times Cited By KSCI : 4  (Citation Analysis)
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