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http://dx.doi.org/10.15207/JKCS.2019.10.4.081

The Convergence Analysis of Microarray-Based Gene Expression by Difference of Culture Environment in Human Oral Epithelial Cells  

Son, Hwa-Kyung (Department of Dental Hygiene, Yeungnam University College)
Publication Information
Journal of the Korea Convergence Society / v.10, no.4, 2019 , pp. 81-89 More about this Journal
Abstract
This study was analyzed about the relationship between culture microenvironment and cell differentiation of HPV 16 E6/E7-transfected immortalized oral keratinocyte(IHOK). By the alteration of culture environment, IHOK-EF and IHOK-EFKGM were obtained, and the modulation of cell properties was observed by cell proliferation assay, immunofluorescence, microarray, and quantitative real-time PCR analysis. IHOK-EF losed the properties of epithelial cells and obtained the properties of mesenchymal cells, and in the result of microarray analysis, genes related to the inhibition of differentiation such as IL6, TWIST1, and ID2 were highly expressed in IHOK-EF. When the culture environment was recovered to initial environment, these changes were recovered partially, presenting the return of genes involved in the inhibition of differentiation such as IL6, and ID2, particularly. This study will contribute to understand adjustment aspect for cell surviving according to the change of culture microenvironment in the study for determining the cell characteristic, and facilitate therapeutic approach for human disease by applying surviving study according to the change of cancer microenvironment.
Keywords
Oral Keratinocyte; Microarray; Differentiation; Microenvironment; Convergence;
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