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http://dx.doi.org/10.11625/KJOA.2022.30.3.409

Combined Application of Bacillus sp. JJ2-01 and Garlic Oil for Controlling Sclerotium rolfsii in Pepper Plants  

Moon, Hye Jeong (국립농업과학원 농업미생물과, 전북대학교 농생물학과)
Ju, Ho-Jong (전북대학교 농생물학과)
Ahn, Seong-Ho (국립농업과학원 농업미생물과)
Song, Jaekyeong (국립농업과학원 농업미생물과)
Sang, Mee Kyung (국립농업과학원 농업미생물과)
Publication Information
Korean Journal of Organic Agriculture / v.30, no.3, 2022 , pp. 409-422 More about this Journal
Abstract
Southern blight caused by Sclerotium rolfsii is a serious soilborne disease in economically important crops including pepper. In this study, we conducted a selection of antagonistic bacterial strains and organic materials to biologically control the disease. Out of 39 strains isolated from soils at Jinju in Korea, strain JJ2-01 showed the highest mycelial growth inhibition; garlic oil among various organic materials significantly reduced disease incidence and severity. When a combination of strain JJ2-01 and garlic oil, or each was drenched into the pepper plants, combined treatment and garlic oil significantly suppressed the disease development, however, acid phosphatase activity in garlic oil-treated plants decreased. In the case of combined treatment, the soil activities did not affect by treatment, while soil urease activity was significantly increased by the combined treatment. Therefore, given soil quality and health for sustainable agriculture, the combination of strain JJ2-01 and garlic acid was an effective application for environmental-friendly control of Southern blight in pepper plants.
Keywords
biocontrol; garlic oil; organic material; Sclerotium rolfsii; soil microbial activity;
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1 Adetunji, A. T., F. B. Lewu, R. Mulidzi, and B. Ncube. 2017. The biological activities of β-glucosidase, phosphatase and urease as soil quality indicators: a review. J. Soil Sci. Plant Nutr. 17: 794-807.   DOI
2 Agrios. G. N. 2005. Plant pathology. 5th ed. Elsevier Academic press. USA. p. 992
3 Camiletti, B. X., C. M. Asensio, L. C. Gadban, M. D. L. P. G. Pecci, M. Y. Conles, and E. I. Lucini. 2016. Essential oils and their combinations with iprodione fungicide as potential antifungal agents against withe rot (Sclerotium cepivorum Berk) in garlic (Allium sativum L.) crops. Ind Crops Prod. 85: 117-124.   DOI
4 Chen, L., Y. D. Wu, X. Y. Chong, Q. H. Xin, D. X. Wang, and K. Bian. 2020. Seed-born endophytic Bacillus velezensis LHSB1 mediate the biocontorl of peanut stem rot caused by Sclerotium rolfsii. J. Appl. Microbiol. 128: 803-813.   DOI
5 Chet, I., A. Ordentlich, R. Shapira, and A. Oppenheim. 1990. Mechanisms of biocontrol of soil-borne plant pathogens by rhizobacteria. Plant and soil. 129: 85-92.   DOI
6 Darma, R., I. M. Purnamasari, D. Agustina, T. E. Pramudito, M. Sugiharti, and A. Suwanto. 2016. A strong antifungal-producing bacteria from bamboo powder for biocontrol of Sclerotium rolfsii in melon (Cucumis melo var. amanta). J. Plant Pathol. Microbiol. 7: 334.
7 De Curtis, F., G. Lima, D. Vitullo, and V. De Cicco. 2010. Biocontrol of Rhizoctonia solani and Sclerotium rolfsii on tomato by delivering antagonistic bacteria through a drip irrigation system. J. Crop Prot. 29: 663-670.   DOI
8 Dusan, F., S. Marian, D. Katarina, and B. Dobroslava. 2006. Essential oils - their antimicrobial activity against Escherichia coli and effect on intestinal cell viability. Toxicol. In Vitro. 20: 1435-1445.   DOI
9 Ma, X., H. Li, J. Zhang, and J. Shen. 2021. Spatiotemporal pattern of acid phosphatase activity in soils cultivated with maize sensing to phosphorus-rich patches. Front. Plant Sci. 12.
10 Mullen, J. 2001. Southern blight, southern stem blight, white mold. Plant Health Instr. DOI: 10.1094/PHI-I-2001-0104-01.   DOI
11 Erkol, D., E. Dane, and C. Eken. 2011. In vitro antagonistic activity of fungi isolated from sclerotia on potato tubers against Rhizoctonia solani. Turk. J. Biol. 35: 457-462.
12 Park, D. W., Y. S. Yang, Y. U. Lee, S. J. Han, H. J. Kim, S. H. Kim, and A. G. Kim. 2021. Pesticide residue and risk assessment from monitoring programs in the largest production area of leafy vegetables in South Korea: a 15-year study. Foods. 10: 425.   DOI
13 Portz, D., E. Koch, and A. J. Slusarenko. 2008. In The Downy Mildews-Genetics, Molecular Biology and Control, Springer, Berlin, Germany, pp. 197-206.
14 Punja, Z. K. 1985. The biology, ecology, and control fo Sclerotium rolfsii. Annu. Rev. Phytopathol. 23: 97-127   DOI
15 Fery, R. L. and P. D. Dukes 2002. Southern blight (Sclerotium rolfsii Sacc.) of cowpea: yield-loss estimates and sources of resistance. J. Crop Prot. 21: 403-408.   DOI
16 Han, J. H., H. J. Jeong, M. R. Lee, S. N. Choi, D. Y. Kim, S. H. Ahn, and J. W. Park. 2020. Insecticidal effect of entomopathogenic fungus, Isaria fumosorosea FG340 to Thrips palmi. Korean J. Pestic. Sci. 24: 374-380   DOI
17 Kandeler, E. and H. Gerber, 1988. Short-term assay of soil urease activity using colorimetric determination of ammonium. Biol. Fertil. Soils. 6: 68-72.
18 Keswani, C., K. Bisen, V. Singh, B. K. Sarma, and H. B. Singh. 2016. Bioformulations: for Sustainable Agriculture, Springer, Berlin, Germany, pp. 35-52.
19 Radice, M., N. R. Maddela, and L. Scalvenzi. 2022. Biological activities of zingiber officinale roscoe essential oil against fusarium spp.: A minireview of a promising tool for biocontrol. Agronomy. 12: 1168.   DOI
20 Saleem, M. S., T. S. Batool, M. F. Akbaar, S. Raza, and S. Shahzad. 2019. Efficiency of botanical pesticides against some pests infesting hydroponic cucumber, cultivated under greenhouse conditions. Egypt. J. Biol. Pest Control 29: 1-7.   DOI
21 Seo, S. T., J. S. Lee, J. H. Park, K. S. Han, and H. I. Jang. 2006. Control of powdery mildew by garlic oil in cucumber and tomato. Res. Plant Dis. 12: 51-54.   DOI
22 Sharf, W., A. Javaid, A. Shoaib, and I. H. Khan. 2021. Induction of resistance in chili against Sclerotium rolfsii by plant-growth-promoting rhizobacteria and Anagallis arvensis. Egypt. J. Biol. Pest Control. 31: 1-11.   DOI
23 Tabatabai, M. A. and J. M. Bremner. 1969. Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol. Biochem. 1: 301-307.   DOI
24 Kumar, A., S. Singh, A. Mukherjee, R. P. Rastogi, and J. P. Verma. 2021. Salt-tolerant plant growth-promoting Bacillus pumilus strain JPVS11 to enhance plant growth attributes of rice and improve soil health under salinity stress. Microbiol. Res. 242: 126616.   DOI
25 Kutawa, A. B., M. D. Danladi, and A. Haruna 2018. Regular article antifungal activity of garlic (Allium sativum) extract on some selected fungi. J. Med. Herbs Ethnomed. 4: 12-14.
26 Kwon, J. H. and C. S. Park. 2004. Stem rot of Capsicum annuum caused by Sclerotium rolfsii in Korea. Res. Plant Dis. 10: 21-24.   DOI
27 Wang, Y., K. Wei, X. Han, D. Zhao, Y. Zheng, J. Chao, and C. S. Zhang. 2019. The antifungal effect of garlic essential oil on Phytophthora nicotianae and the inhibitory component involved. Biomolecules. 9: 632.   DOI
28 Lin, Y. C., K. R. Chung, and J. W. Huang. 2020. A synergistic effect of chitosan and lactic acid bacteria on the control of cruciferous vegetable diseases. Plant Pathol. J. 36: 157.   DOI
29 Khan, N., M. Maymon, and A. M. Hirsch. 2017. Combating Fusarium infection using Bacillus-based antimicrobials. Microorganisms. 5: 75.   DOI
30 Turkmen, M., M. Kara, H. Maral, and S. Soylu. 2021. Determination of chemical component of essential oil of Origanum dubium plants grown at different altitudes and antifungal activity against Sclerotinia sclerotiorum. J. Food Process. Preserv. e15787.
31 Wu, J., B. Yang, X. Zhang, A. G. Cuthbertson, and S. Ali. 2021. Synergistic interaction between the entomopathogenic fungus Akanthomyces attenuatus (Zare & Gams) and the botanical insecticide matrine against Megalurothrips usitatus (Bagrall). J. Fungi. 7: 536.   DOI
32 Xie, C. and G. Vallad. 2010. Integrated management of southern blight in vegetable production. EDIS. 2010.
33 Xu, M., X. Zhang, J. Yu, Z. Guo, J. Wu, X. Li, Y. Chi, and S. Wan. 2020. Biological control of peanut southern blight (Sclerotium rolfsii) by the strain Bacillus pumilus LX11. Biocontrol Sci. Technol. 30: 485-489.   DOI
34 Yeon, I. K., C. K. Shim, M. J. Kim, J. M. Park, W. H. Jung, H. W. Do, and S. H. Park. 2020. Research status of environmental friendly management for korean melon powdery mildew. Korean J. Pestic. Sci. 24: 136-147.   DOI