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http://dx.doi.org/10.14479/jkoos.2015.20.3.285

Development of Hyaluronic Acid-Functionalized Hydrogel Lens and Characterization of Physical Properties and Lysozyme Adsorption  

Lim, Hwa-lim (Dept. of Optometry and Optic Science, Dongshin University)
Kim, Ho-joong (Dept. of Chemistry, Chosun University)
Jun, Jin (Dept. of Optometry and Optic Science, Dongshin University)
Publication Information
Journal of Korean Ophthalmic Optics Society / v.20, no.3, 2015 , pp. 285-291 More about this Journal
Abstract
Purpose: The hydrogel lenses have been functionalized with HA(Hyaluronic Acid) using two different methods: construction of an IPN(Interpenetrating Polymer Networks) and formation of CCB(Chemical Covalent Bonding). The lysozyme adsorption and physical properties such as optical transmittance and water content of the hydrogel lenses have been investigated in order to determine whether method is suitable for the application potentials in contact lens industry. Methods: HA have been added to the hydrogel lenses prepared in the Lab using the two different method, e.g. IPN and CCB. The optical transmittance was measured in the wavelength range of 300~800 nm. The water content was measured by the gravimetric method using 0.9% NaCl saline solution. The amounts of adsorbed lysozyme on the contact lenses was analyzed by HPLC after incubation for 12h in artificial tears. Results: The water content of the HA added hydrogel contact lenses was increased, and the lens made by IPN method showed higher water content than the lens made by CCB method. The optical transmittance was over 90% both before and after addition of HA. Comparing the lysozyme adsorption reduction ratio, contact lens manufactured by IPN method was 60.0%, and the lens made by CCB method was 40.4%. Conclusions: CCB method is appropriate to distribute the functional material evenly throughout the lens, whereas IPN method is effective for the case of giving the functionality on the lens surface without phase separation.
Keywords
Contact lens; Hyaluronic acid; IPN; Chemical Covalent Bonding; Lysozyme adsorption; Water contents; Hydrogel;
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Times Cited By KSCI : 5  (Citation Analysis)
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1 Takanori S, Tatsuro G, Norihiko M, Madoka T, Kazuhiko I. Super-hydrophilic silicone hydrogels with interpenetrating poly(2-methacryloyloxyethyl phosphorylcholine) networks. Science Direct Biomaterials. 2010;31:3274-3280.
2 Doerte L, Lyndon J. Protein deposition on contact lenses: The past, the present, and the future. Contact Lens & Anterior Eye. 2012;35(2):53-64.   DOI
3 Garrett Q, Laycock B, Garrett RW. Hydrogel lens monomer constituents modulate protein sorption. Ophthalmology & Visual Science. 2000;41(7):1687-1695.
4 Andrea W, Doerte L, Adrienne B, Lyndon J, Heather S. Hyaluronic acid as an internal wetting agent on model DMAA/TRIS contact lenses. Journal of Biomaterials applications. 2011;27(4):423-432.   DOI
5 Sung YJ, Ryu GC, Jun J. Adsorption properties of the lysozyme and albumin with physicochemical properties of the contact lens. J Korean Ophthalmic Opt. Soc. 2013; 18(3):261-270.   DOI
6 Lord MS, Stenzel MH, Simmons A, Milthorpe BK. Lysozyme interaction with poly(HEMA)-based hydrogel. Biomaterials. 2006;27(8):1341-1345.   DOI
7 Prager MD, Quintana RP. Radiochemical studies on contact lens soilation. I. Lens uptake of 14C-lysozyme from simple and complex artificial tear solutions. J Biomed Mater Res. 1997;36(1):119-124.   DOI
8 Sack RA, Jones B, Antignani A, Libow R, Harvey H. Specificity and biological activity of the protein deposited on the hydrogel surface. Invest Ophthalmol Visual Science. 1987;28(5):842-849.
9 Hume E, Sack R, Stapleton F, Willcox M. Induction of cytokines from polymorphononuclear leukocytes and epithelial cells by ocular isolates of Serratia Marcescens. Ocular Immunol Inflammation. 2004;12(4):287-295.   DOI
10 Bengani LC, Leclerc J, Chauhan A. Lysozyme transport in p-HEMA hydrogel contact lenses, J Colloid Interface Sci. 2012;386(1):441-450.   DOI
11 Rah MJ. A review of hyaluronan and its ophtjalmic applications. American Optometric Association. 2011;82:38-43.   DOI
12 Kim HJ, Kim SH, Kim JM. A study on improvement of wettability and comfort in contact lens with hyaluronic acid. J Korean Ophthalmic Opt Soc. 2011;16(3):255-264.
13 Mark VB, Lyndon J, Heather S. Hyaluronic acid containing hydrogels for the reduction of protein adsorption. Science Direct Biomaterials. 2008;29(7):780-789.
14 Scheuer CA, Fridman KM, Barniak VL, Burke SE, Venkatesh S, Venkatesh S. Retention of conditioning agent hyaluronan on hydrogel contact lenses. Contact Lens & Anterior Eye. 2010;33S:S2-S6.
15 Keith D, Hong B, Christensen M. A novel procedure for the extraction of protein deposits from soft hydrophilic contact lenses for analysis. Current Eye Research. 1997; 16(5):503-510.   DOI
16 Zhenyu F, Chunlian H, Hebing L, Chong Y, Limiao C, Jianhan H, et al. A novel hydrophilic-hydrophobic magnetic interpenetrating polymer networks(IPNs) and its adsorption towards salicylic acid from aqueous solution. Chemical Engineering Journal. 2015;279:250-257.   DOI
17 Jeong KS, Kim HJ, Lim HL, Ryu GC, Seo ES, You NH, et al. Synthesis and biocompatibility of silicone hydrogel functionalized with polysaccharide. Bulletin of the Korean Chemical Society. 2015;36(6):1649-1653.   DOI
18 Choi JY, Park JS, Kim SR, Park MJ. The change in refractive powers of soft contact lenses caused by the deposition of tear proteins. J Korean Ophthalmic Opt Soc. 2011;16(4): 383-390.
19 Cho NR, Ryu GC, Jun J. The properties of hyaluronan addition on the protein adsorption at the silicone hydrogel contact lens. Korean J Vis Sci. 2014;16(1):99-109.
20 van Beek M, Weeks A, Jones L, Sheardown H. Immobilized hyaluronic acid containing model silicone hydrogels reduce protein adsorption. J Biomater Sci Polym Ed. 2008;19(11):533-537.
21 Norde W, Lyklema J. Why proteins preferinterfaces. J Biomater Sci Polym Ed. 1991;2(3):183-202.   DOI