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http://dx.doi.org/10.14479/jkoos.2015.20.1.51

Cytotoxicity of Multipurpose Contact Lens Solutions on the Cultured Corneal Epithelial Cells Evaluated by Image Analysis  

Kim, Nam-Youl (Graduate School of Public Health Science, Eulji University)
Lee, Koon-Ja (Graduate School of Public Health Science, Eulji University)
Publication Information
Journal of Korean Ophthalmic Optics Society / v.20, no.1, 2015 , pp. 51-60 More about this Journal
Abstract
Purpose: To determine the effect of marketed multipurpose contact lens solutions (MPSs) on human corneal epithelial cells (HCEpiCs) toxicity by using image analysis. Methods: HCEpiCs were exposed six MPSs (product A-F) at 0.05~50% for 2h, 12h, 24h, and 48h respectively. HCEpiCs were fixed and stained with Draq5 after exposure with MPSs, and the cell viability and apoptosis were evaluated by using confocal microscope and ImageXpress UltraTM. Results: Viabilities of HCEpiCs exposed to MPS A-F for a 2h were not affected, while reductions (52~75%) in cell viability over a 12h exposure of MPS B, MPS C, MPS D and MPS F, and significant more reductions (29~73%) over a 24h and 48h-exposure. Apoptosis of HCEpiC was not affect over a 12h MPS exposure, however was significantly increased (199~526%) over 24h and 48h MPS exposure. Among the products MPS D, E and F reduced viability of HCEpiCs and apoptosis increased more than MPS A (p<0.05). Conclusions: Lower concentration of MPSs have not an cytotoxic effect on HCEpiCs, however higher concentration of MPSs induce apoptosis and reduce viability of HCEpiCs. Therefore, it need to develop MPS having antimicrobial effectiveness with low cytotoxicity.
Keywords
Human corneal epithelial cell (HCEpiC); Multi purpose solution; Apoptosis; Cytotoxicity; Image analysis;
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