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A Study of Sterilization Effect of Long-wavelength UVA-LED Irradiation on Bacteria Causing Eye Diseases  

Lee, Cheol-Woo (Department of Ophthalmic Optics Dong-Shin University)
Jeong, Kyeong-In (Department of Ophthalmic Optics Dong-Shin University)
Hwang, Kwang-Ha (Department of Ophthalmic Optics Dong-Shin University)
Lee, Seok-Ju (Department of Ophthalmic Optics Dong-Shin University)
Yoo, Geun-Chang (Department of Ophthalmic Optics Dong-Shin University)
Publication Information
Journal of Korean Ophthalmic Optics Society / v.17, no.1, 2012 , pp. 99-105 More about this Journal
Abstract
Purpose: The purpose of this study is to demonstrate inactivation effect of UVA-LED ultraviolet radiation upon Pseudomonas aeruginosa and Staphylococcus aureus which are the major bacteria causing eye diseases. Methods: The small sterilization device was made using UVA-LED of 400 nm. After Pseudomonas aeruginosa was diluted to $10^{-7}$ and Staphylococcus aureus to $10^{-5}$ and diluted solutions were put onto each liquid medium. They were irradiated by 400 nm of UVA for different amount of time; 15 min, 30 min, 60 min, 120 min, 240 min, 360 min and 480 min each. Results: The data from sterilization test was solved to regression line equation and the target log inactivation was obtained. The 3 log inactivation UV irradiation value of Pseudomonas aeruginosa was 54,847 UV dose ($mJ/cm^2$) and irradiation time was 135.42 min while the 3 log inactivation of Staphylococcus aureus was 39,066 UV dose ($mJ/cm^2$) and irradiation time was 98.72 min. Conclusions: The inactivation effect of sterilization method using 400 nm of UVA-LED upon Pseudomonas aeruginosa and Staphylococcus aureus has been verified and it is considered as a useful method in inactivating the contact lenses.
Keywords
UVA-LED; Pseudomonas aeruginosa; Staphylococcus aureus; Inactivation effect (Sterilization effect);
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  • Reference
1 EPA., "Ultraviolet disinfection guidance manual", Office of Water, Washington D. C., USA, pp. 35-59(2003).
2 Zhao J., Krishna V., Hua B., Moudgil B., and Koopman B., "Effect of UVA irradiance on photocatalytic and UVA inactivation of bacillus cereus spores", J. Photochem. Photobiol. B, 94(2):96-100(2009).   DOI   ScienceOn
3 Bosshard F., Bucheli M., Meur Y., and Egli T., "The respiratory chain is the cell's achilles' heel during UVA inactivation in escherichia coli", Microbiology, 156(pt7):2006-2015(2010).   DOI   ScienceOn
4 Yoshimura-Mishima M., Akamatsu H., Namura S., and Horio T., "Suppressive effect of ultraviolet (UVB and PUVA) radiation on superantigen production by staphylococcus aureus", Journal of Dermatological Science, 19(1):31-36(1999).   DOI   ScienceOn
5 Lopez M. A., and Palou E., "Ultraviolet light and food preservation. In: Novel Food Processing Technologies", Barbosa-Canovas G. V,, Tapia M. S., and Cano M. P., 2nd Ed., CRC Press, Inc., Boca Raton, FL., USA, 405-421(2005).
6 Wurtele M. A., Kolbe T., Lipsz M., Kulberg A., Weyers M., Kneissl M., and Jekel M., "Application of GaN-based ultraviolet-C light emitting diodes - UV LEDs- for water disinfection", Water res., 45(3):1481-1489(2011).   DOI   ScienceOn
7 Bartolomei A., Alcaraz L., Bottone E., Asbell P., Press L., and Solomon J., et al., "Clinical evaluation of purilens, an ultraviolet light contact lens care system", CLAO J., 20(1):23-26(1994).
8 Harris M. G., Fluss L., Lem A., and Leong H., "Ultraviolet disinfection of contact Lenses", Optom. Vis. Sci., 70(10): 839-842(1993).   DOI   ScienceOn
9 Harris M. G., Buttino L. M., Chan J. C., and Wang M., "Effects of ultraviolet radiation on contact lens parameters", Optom. Vis. Sci., 70(9):739-42(1993).   DOI   ScienceOn
10 Berney M., Weilenmann H. U., Ihssen J., Bassin C., and Egli T., "Specific growth rate determines the sensitivity of escherichia coli to thermal, UVA, and solar disinfection", Appl. Environ. Microbiol., 72(4):2586-2593(2006).   DOI   ScienceOn
11 Mori M., Hamamoto A., Takahashi A., Nakano M., Wakikawa N., and Tachibana S., et al., "Development of a new water sterilization device with a 365 nm UV-LED", Med. Biol. Eng. Comput., 45(12):1237-1241(2007).   DOI   ScienceOn
12 Hamamoto A., Mori M., Takahashi A., Nakano M., Wakikawa N., and Akutagawa M., et al., "New water disinfection system using UVA light-emitting diodes", J. Appl. Microbiol., 103(6):2291-2298(2007).   DOI   ScienceOn
13 Lian X., Tetsutani K., Katayama M., Nakano M., Mawatari K., and Harada N., et al., "New colored beverage disinfection system using UV-A light-emitting diodes", Biocontrol Sci., 15(1):33-37(2010).   DOI   ScienceOn
14 차흥원, 한태원, 한영호, 김재찬, "콘택트렌즈 및 콘택트렌즈 저장 용기의 오염도(콘택트렌즈와 연관된 감염성각막염 환자를 대상으로)", 대한안과학회지, 41(2): 349-355(2000).
15 오염상, 성경림, 차흥원, "Photorefractive keratectomy(PRK) 후 착용한 치료용 콘택트렌즈의 오염", 대한안과학회지, 44(2):309-314(2003).
16 유근창, 김인숙, "콘택트렌즈 착용에 따른 사용자 실태 조사", 한국안광학회지, 7(1):15-20(2002).
17 최태훈, 김효명, 차흥원, 김재찬, 김만수, 이하범, "한국 콘택트렌즈실태조사", 대한안과학회지, 45(11):1833-1841(2004).
18 김소라, 신상목, 박종애, 박미정, "소프트 콘택트렌즈 착용기간과 보관기간에 따른 보관 용기의 오염도 및 보관용기 관리 실태", 한국안광학회지, 16(2):135-145(2011).
19 김인숙, 전창진, "천연보존제와 화학보존제가 가토안의 각막 상피, 내피에 미치는 영향(주사전자현미경적 관찰)", 한국전자현미경학회지, 35(3):129-134(2005).
20 이지영, 서승교, 성덕용, 육도진, 이군자, 정재용, "다목적 용액에서 천연보존제로서의 Allyl 유도체의 항균활성", 한국안광학회지, 14(3):95-102(2009).
21 한선희, 김봉환, "안질환세균에 대한 도꼬마리 추출물의 항균 활성", 한국안광학회지, 13(2):69-73(2008).
22 김인숙, 유근창, "콘택트렌즈 보존제 $H_2O_2$와 자몽씨 추출물의 세포 독성 비교 연구", 한국안광학회지, 9(1):173-180(2004).
23 Girard P. M., Francesconi S., Pozzebon M., Graindorge D., Rochette P., Drouin R., and Sage E., "UVA-induced damage to DNA and proteins: direct versus indirect photochemical processes", Journal of Physics: Conference Series, 261(1):12002-12011(2011).
24 Tyrrell R. M., "Induction of pyrimidine dimers in bacterial DNA by 365 nm radiation", Photochem. Photobiol., 17(1):69-73(1973).   DOI   ScienceOn
25 Vilhunen S., Sarkka H., and Sillanpaa M., "Ultraviolet light-emitting diodes in water disinfection", Environ. Sci. Pollut. Res. Int., 16(4):439-442(2009).   DOI   ScienceOn
26 김재용, 박혜련, 조영걸, 김유겸, 한태원, 한영호, 차흥원, "다목적 콘택트렌즈 세정액의 항녹농균 효과 비교", 대한안과학회지, 43(1):178-188(2002).
27 김성홍, "자외선을 이용한 먹는 물 소독 방법에 관한 고찰", 공학기술논문지, 1(2):321-326(2008).