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Selective Accumulation of Rhodamine B in Müller cells in Rabbit Retina  

Kwon, Oh Ju (Department of Ophthalmic Optics, Busan College of Information Technology)
Lee, Eun Shil (Department of Biology, College of Natural Science, Kyungpook National University)
Jeon, Chang Jin (Department of Biology, College of Natural Science, Kyungpook National University)
Publication Information
Journal of Korean Ophthalmic Optics Society / v.16, no.1, 2011 , pp. 91-95 More about this Journal
Abstract
Purpose: In this study, we investigated the dye to staining for selective accumulation in rabbit retina. Methods: Rhodamine B was injected into the vitreous body in rabbit. After 24 h, the isolated retina was checked condition of cell staining on the microscope. We used conventional immunocytochemical techniques for recognizing cell type. Results: Well-labeled nuclei were seen in the middle of the inner nuclear layer of the rabbit retina. The number and distrbution of the accumulating cells were similar to those of the m$\ddot{u}$ller glia. To identify m$\ddot{u}$ller cell, we used antibody directed against vimentin. Rhodamine B-immunoreactive nuclei also were labeled with antivimentin antibody. We found that Rhodamine B was accumulated selectively in retinal m$\ddot{u}$ller cell. Conclusions: Specific accumulation in rabbit retinal m$\ddot{u}$ller cell occurred when Rhodamine B was applied to living retina.
Keywords
Cell staining; M$\ddot{u}$ller cell; Rabbit retinal cell; Rhodamine B;
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