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http://dx.doi.org/10.5293/KFMA.2011.14.2.017

Geometric Optimization of a Microchannel for the Improvement of Temperature Gradient Focusing  

Han, Tae-Heon (인하대학교 기계공학과)
Kim, Sun-Min (인하대학교 기계공학과)
Publication Information
The KSFM Journal of Fluid Machinery / v.14, no.2, 2011 , pp. 17-24 More about this Journal
Abstract
Temperature gradient focusing (TGF) of analytes via Joule heating is achieved when electric field is applied along a microchannel of varying width. The effect of varying width of the microchannel for the focusing performance of the device was numerically studied. The governing equations were implemented into a quasi-1D numerical model along a microchannel. The validity of the numerical model was verified by a comparison between numerical and experimental results. The distributions of temperature, velocity, and concentration along a microchannel were predicted by the numerical results. The narrower middle width and wider outside width of the channel having the fixed length contribute to improve the focusing performance of the device. However, too narrow middle width of the channel generates a higher temperature which can cause the problems including sample denaturation and buffer solution boiling. Therefore, the channel geometry should be optimized to prevent these problems. The optimal widths of the microchannel for the improvement on TGF were proposed and this model can be easily applied to lab-on-a-chip (LOC) applications where focusing is required based on its simple design.
Keywords
Temperature gradient focusing; Joule heating; Geometric optimization; quasi-1D numerical model;
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1 Sommer, G. J., Kim, S. M., Littrell, R. J. and Hasselbrink, E. F., 2007, “Theoretical and Numerical Analysis of Temperature Gradient Focusing via Joule Heating,” Lab on a Chip, Vol. 7, No. 7, pp. 898-907.   DOI
2 Wooding, R. A., 1960, “Instability of a Viscous Liquid of Variable Density in a Vertical Hele-Shaw Cell,” Journal of Fluid Mechanics, Vol. 7, pp. 501-515.   DOI
3 Tang, G. Y., Yan, D. G., Yang, C., Gong, H. Q., Chai, J. C. and Lam, Y. C., 2006, “Assessment of Joule Heating and its Effects on Electroosmotic Flow and Electrophoretic Transport of Solutes in Microfluidic Channels,” Electrophoresis, Vol. 27, No. 3, pp. 628-639.   DOI
4 Tang, G. Y., Yang, C., Gong, H. Q., Chai, J. C. and Lam, Y. C., 2006, “Numerical Simulation of Joule Heating Effect on Sample Band Transport in Capillary Electrophoresis,” Analytica Chimica Acta, Vol. 561, No. 1-2, pp. 138-149.   DOI
5 Probstein, R. F., 2003, Physicochemical Hydrodynamics, John Wiley and Sons, Inc., New York, USA.
6 Incropera, F. P. and DeWitt, D. P., 1996, Fundamentals of Heat and Mass Transfer, John Wiley and Sons, Inc., New York, USA.
7 Allen, M. W., 2007, Thermal Denaturation of DNA in Semimicro Cells, Thermo Fisher Scientific Inc., Madison, WI, USA.
8 Koegler, W. S. and Ivory, C. F., 1996, “Focusing Proteins in an Electric Field Gradient,” Journal of Chromatography A, Vol. 726, No. 1-2, pp. 229-236.   DOI
9 Petsev, D. N., Lopez, G. P., Ivory, C. F. and Sibbett, S. S., 2005, “Microchannel Protein Separation by Electric Field Gradient Focusing,” Lab on a Chip, Vol. 5, No. 6, pp. 587-597.   DOI
10 Righetti, P. G. and Bossi, A., 1998, “Isoelectric Focusing of Proteins and Peptides in Gel Slabs and in Capillaries,” Analytica Chimica Acta, Vol. 372, No. 1-2, pp. 1-19.   DOI
11 Gebauer, P. and Bocek, P., 2002, “Recent Progress in Capillary Isotachophoresis,” Electrophoresis, Vol. 23, No. 22-23, pp. 3858-3864.   DOI
12 Ross, D. and Locascio, L. E., 2002, “Microfluidic Temperature Gradient Focusing,” Analytical Chemistry, Vol. 74, No. 11, pp. 2556-2564.   DOI
13 Balss, K. M., Ross, D., Begley, H. C., Olsen, K. G. and Tarlov, M. J., 2004, “DNA Hybridization Assays Using Temperature Gradient Focusing and Peptide Nucleic Acids,” Analytical Chemistry, Vol. 126, No. 41, pp. 13474-13479.   DOI
14 Khandurina, J., Jacobson, S. C., Waters, L. C., Foote, R. S. and Ramsey, J. M., 1999, “Microfabricated Porous Membrane Structure for Sample Concentration and Electrophoretic Analysis,” Analytical Chemistry, Vol. 71, No. 9, pp. 1815-1819.   DOI
15 Balss, K. M., Vreeland, W. N., Phinney, K. W. and Ross, D., 2004, “Simultaneous Concentration and Separation of Enantiomers with Chiral Temperature Gradient Focusing,” Analytical Chemistry, Vol. 76, No. 24, pp. 7243-4249.   DOI
16 Balss, K. M., Vreeland, W. N., Howell, P. B., Henry, A. C. and Ross, D., 2004, “Micellar Affinity Gradient Focusing; A New Method for Electrokinetic Focusing,” Journal of the American Chemical Society, Vol. 126, No. 7, pp. 1936-1937.   DOI
17 Kim, S. M., Sommer, G. J., Burns, M. A., and Hasselbrink, E. F., 2006, “Low-Power Concentration and Separation Using Temperature Gradient Focusing via Joule Heating,” Analytical Chemistry, Vol. 78, No. 23, pp. 8028-8035.   DOI
18 Kim, S. M., Burns, M. A. and Hasselbrink, E. F., 2006, “Electrokinetic Protein Preconcentration Using a Simple Glass/Poly(dimethylsiloxane) Microfluidic Chip,” Analytical Chemistry, Vol. 78, No. 14, pp. 4779-4785.   DOI
19 Hatch, A. V., Herr, A. E., Throckmorton, D. J., Brennan, J. S. and Singh, A. K., 2006, “Integrated Preconcentration SDS-PAGE of Proteins in Microchips Using Photopatterned Cross-Linked Polyacrylamide Gels,” Analytical Chemistry, Vol. 78, No. 14, pp. 4976-4984.   DOI
20 Wang, Y. C., Stevens, A. L. and Han, J. Y., 2005, “Million-fold Preconcentration of Proteins and Peptides by Nanofluidic Filter,” Analytical Chemistry, Vol. 77, No. 14, pp. 4293-4299.   DOI
21 Yu, C., Davey, M. H., Svec, F. and Frechet, J. M. J., 2001, “Monolithic Porous Polymer for On-Chip Solid-Phase Extraction and Preconcentration Prepared by Photoinitiated in Situ Polymerization within a Microfluidic Device,” Analytical Chemistry, Vol. 73, No. 21, pp. 5088-5096.   DOI   ScienceOn