Browse > Article

The effects of Pueraria lobata extract on gene expression in liver tissue of rat with estrogen-deficient obesity  

Shin, Yoon Sang (College of Korean Medicine, Gachon University)
Hwang, Gwi Seo (College of Korean Medicine, Gachon University)
Publication Information
Journal of Society of Preventive Korean Medicine / v.18, no.3, 2014 , pp. 117-128 More about this Journal
Abstract
Objective : It is known that Pueriaria lobata has an anti-osteoporetic effect, anti-cancer effect, anti-pyretic effect, and anti-diabetic effect. The aim of this study was to evaluate anti-obesity effect of Pueriaria lobata extract (PLE), and elucidate the effect of it on gene expression related to lipid metabolism. Method : The experiments were performed with the use of ovariectomized rats as estrogen-deficient obesity model. They were grouped NC (normal control), OC (estrogen-deficient control), PLH (100mg/kg of PLE), PLL (20mg/kg). PLE was orally administered for 6 weeks. Body weights and serum lipid level were estimated, and real-time PCR was performed to investigate the effect of PLE on gene expression in liver. Results : PLE decreased the body weight and serum cholesterol and triglyceride, but increased HDL-cholesterol. And PLE increased leptin, CYP27, CPT1, CYP8B1, ACAT2, LDLR, and SCD1, but reduced $PPAR{\gamma}$, PGC1A, HMG-CoA-R, ACAT1, SCD1, and APoB gene expression in liver tissue of rat with estrogen-deficient obesity. Conclusion : It is concluded that Pueriaria lobata reduced body weight, and its effect was expressed by regulation of gene expression related to lipid metabolism in rats with estrogen-deficient obesity.
Keywords
Pueriaria lobata; obesity; estrogen; gene expression;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Somogyi V, Gyorffy A, Scalise TJ, Kiss DS, Goszleth G, Bartha T, Frenyo VL, Zsarnovszky A. Endocrine factors in the hypothalamic regulation of food intake in females: a review of the physiological roles and interactions of ghrelin, leptin, thyroid hormones, oestrogen and insulin. Nutr Res Rev. 2011;22:1-23.
2 Cooke PS, Naaz A. Role of estrogens in adipocyte development and function. Exp Biol Med (Maywood). 2004;229(11):1127-35.
3 Tchernof A, Despres JP. Sex steroid hormones, sex hormone-binding globulin, and obesity in men and women. Horm Metab Res. 2000; 32:526-36.   DOI   ScienceOn
4 Ouchi N, Parker JL, Lugus JJ, Walsh K. Adipokines in inflammation and metabolic disease. Nat Rev Immunol. 2011;11(2):85-97.   DOI   ScienceOn
5 정보석, 신민교, 향약대사전, 영림사, 1989;704-706.
6 Chen G, Zhang J, Jiannong Y. Determination of puerarin, daidzein and rutin in Pueraria lobata (Wild.) Ohwi by capillary electrophoresis with electrochemical detection. Chromatogr A. 2001;923(1-2):255-62.   DOI
7 Wang X, Wu J, Chiba H, Umegaki K, Yamada K, Ishimi Y. Puerariae radix prevents bone loss in ovariectomized mice. J Bone Miner Metab. 2003;21(5):268-75.   DOI   ScienceOn
8 Hsu FL, Liu IM, Kuo DH, Chen WC, Su HC, Cheng JT. Antihyperglycemic effect of puerarin in streptozotocin-induced diabetic rats. J Nat Prod. 2003;66(6):788-92.   DOI   ScienceOn
9 Qi W, Weber CR, Wasland K, Savkovic SD. Genistein inhibits proliferation of colon cancer cells by attenuating a negative effect of epidermal growth factor on tumor suppressor FOXO3 activity. BMC Cancer. 2011;11(1):219.   DOI
10 Kang SC, Lee CM, Choi H, Lee JH, Oh JS, Kwak JH, Zee OP. Evaluation of oriental medicinal herbs for estrogenic and antiproliferative activities. Phytother Res. 2006;20 (11):1017-9.   DOI   ScienceOn
11 Mendelsohn, M.E., Karas, R.H. Mechanisms of disease: the protective effects of estrogen on the cardiovascular system. N. Engl. J. Med. 1999;340:1801-1811.   DOI   ScienceOn
12 Rasouli N, Kern PA. Adipocytokines and the metabolic complications of obesity. J Clin Endocrinol Metab. 2008;93(11 Suppl 1):S64-73.   DOI
13 Hsu WY, Chao YW, Tsai YL, Lien CC, Chang CF, Deng MC, Ho LT, Kwok CF, Juan CC. Resistin induces monocyte-endothelial cell adhesion by increasing ICAM-1 and VCAM-1 expression in endothelial cells via p38MAPKdependent pathway. J Cell Physiol. 2011;226 (8):2181-8.   DOI   ScienceOn
14 St Clair RW. Effects of estrogens on macrophage foam cells: a potential target for the protective effects of estrogens on atherosclerosis. Curr Opin Lipidol. 1997;8(5):281-6.   DOI
15 Homma H, Kurachi H, Nishio Y, Takeda T, Yamamoto T, Adachi K, Morishige K, Ohmichi M, Matsuzawa Y, Murata Y. Estrogen suppresses transcription of lipoprotein lipase gene. Existence of a unique estrogen response element on the lipoprotein lipase promoter. J Biol Chem. 2000;275(15):11404-11.   DOI   ScienceOn
16 Gao S, Kinzig KP, Aja S, Scott KA, Keung W, Kelly S, Strynadka K, Chohnan S, Smith WW, Tamashiro KL, Ladenheim EE, Ronnett GV, Tu Y, Birnbaum MJ, Lopaschuk GD, Moran TH. Leptin activates hypothalamic acetyl-CoA carboxylase to inhibit food intake. Proc Natl Acad Sci U S A. 2007;104(44): 17358-63.   DOI   ScienceOn
17 Noh JR, Kim YH, Hwang JH, Gang GT, Yeo SH, Kim KS, Oh WK, Ly SY, Lee IK, Lee CH. Scoparone inhibits adipocyte differentiation through down-regulation of peroxisome proliferators-activated receptor ${\gamma}$ in 3T3-L1 preadipocytes. Food Chem. 2013;141(2):723-30   DOI
18 Gastaldi G, Russell A, Golay A, Giacobino JP, Habicht F, Barthassat V, Muzzin P, Bobbioni-Harsch E. Upregulation of peroxisome proliferator-activated receptor gamma coactivator gene (PGC1A) during weight loss is related to insulin sensitivity but not to energy expenditure. Diabetologia. 2007;50(11): 2348-55.   DOI
19 Jo Y, Debose-Boyd RA. Control of cholesterol synthesis through regulated ER-associated degradation of HMG CoA reductase. Crit Rev Biochem Mol Biol. 2010;45(3):185-98.   DOI   ScienceOn
20 Horton JD, Goldstein JL, Brown MS. SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver. J Clin Invest. 2002;109(9):1125-31.   DOI
21 Lundell K, Wikvall K. Species-specific and age-dependent bile acid composition: aspects on CYP8B and CYP4A subfamilies in bile acid biosynthesis. Curr Drug Metab. 2008;9(4):323-31.   DOI
22 Meir K, Kitsberg D, Alkalay I, Szafer F, Rosen H, Shpitzen S, Avi LB, Staels B, Fievet C, Meiner V, Bjorkhem I, Leitersdorf E. Human sterol 27-hydroxylase (CYP27) overexpressor transgenic mouse model. Evidence against 27-hydroxycholesterol as a critical regulator of cholesterol homeostasis. J Biol Chem. 2002; 277(37):34036-41.   DOI
23 Khor VK, Dhir R, Yin X, Ahima RS, Song WC. Estrogen sulfotransferase regulates body fat and glucose homeostasis in female mice. Am J Physiol Endocrinol Metab. 2010;299(4):E657-64.   DOI
24 Yang L, Yang JB, Chen J, Yu GY, Zhou P, Lei L, Wang ZZ, Cy Chang C, Yang XY, Chang TY, Li BL. Enhancement of human ACAT1 gene expression to promote the macrophage-derived foam cell formation by dexamethasone. Cell Res. 2004;14(4):315-23.   DOI
25 Song BL, Wang CH, Yao XM, Yang L, Zhang WJ, Wang ZZ, Zhao XN, Yang JB, Qi W, Yang XY, Inoue K, Lin ZX, Zhang HZ, Kodama T, Chang CC, Liu YK, Chang TY, Li BL. Human acyl-CoA:cholesterol acyltransferase 2 gene expression in intestinal Caco-2 cells and in hepatocellular carcinoma. Biochem J. 2006;394(Pt 3):617-26.   DOI
26 Contois JH, McConnell JP, Sethi AA, Csako G, Devaraj S, Hoefner DM, Warnick GR, Apolipoprotein B and cardiovascular disease risk: position statement from the AACC Lipoproteins and Vascular Diseases Division Working Group on Best Practices. Clin Chem. 2009;55(3):407-19.   DOI
27 Poirier Y, Antonenkov VD, Glumoff T, Hiltunen JK. Peroxisomal beta-oxidation-a metabolic pathway with multiple functions. Biochim Biophys Acta. 2006;1763(12):1413-26.
28 Navarra T, Del Turco S, Berti S, Basta G. The lectin-like oxidized low-density lipoprotein receptor-1 and its soluble form: cardiovascular implications. J Atheroscler Thromb. 2010;17(4):317-31.   DOI   ScienceOn
29 Brown JM, Rudel LL. Stearoyl-coenzyme A desaturase 1 inhibition and the metabolic syndrome: considerations for future drug discovery. Curr Opin Lipidol. 2010;21(3):192-7.   DOI   ScienceOn
30 Karasu T, Marczylo TH, Maccarrone M, Konje JC. The role of sex steroid hormones, cytokines and the endocannabinoid system in female fertility. Hum Reprod Update. 2011; 17(3):347-61.   DOI