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Immune enhancement effect of Rubus coreanus Miquel (RC) (30% EtOH extract) in RAW264.7 cells  

Cho, Jae-Kyung (Department of Preventive Medicine, College of Korean Medicine, Kyunghee University)
Choi, Han-Seok (Department of Preventive Medicine, College of Korean Medicine, Kyunghee University)
Kim, Min-Su (Department of Preventive Medicine, College of Korean Medicine, Kyunghee University)
Kim, Young-Gook (Department of Preventive Medicine, College of Korean Medicine, Kyunghee University)
Kim, Chi-Hong (Department of Preventive Medicine, College of Korean Medicine, Kyunghee University)
Shin, Yong-Cheol (Department of Preventive Medicine, College of Korean Medicine, Kyunghee University)
Ko, Seong-Gyu (Department of Preventive Medicine, College of Korean Medicine, Kyunghee University)
Publication Information
Journal of Society of Preventive Korean Medicine / v.16, no.3, 2012 , pp. 155-165 More about this Journal
Abstract
Objective : The object of this study is to confirm the immune enhancement effect of Rubus coreanus Miquel (RC) (30% EtOH extract) on RAW264.7 mouse macrophage cell line. Methods : RAW264.7 cells were treated with $10-500{\mu}g/mL$ RC for 24 hours. Cell viability was then measured using WST assays. Levels of intracellular NO and ROS were measured by Griess reagent and DCFH-DA staining respectively. Levels of iNOS and COX-2 mRNA was determined by RT-PCR. Secretion levels of IL-$1{\beta}$ and IL-6 cytokines were evaluated by sandwich ELISA assay. Western blot analysis was performed to measure the levels of intracellular molecules related to MAPKs pathways. Results : RC suppressed the growth of RAW264.7 cells. RC increased the production of NO and ROS. RC increased the mRNA and protein levels of COX-2 and iNOS. RC augmented the levels of secreted IL-$1{\beta}$ and IL-6 cytokines. RC increased MAPKs phosphorylation. Conclusion : In summary, our result shows that RC has inflammatory effect increasing the levels of NO, ROS and secreted cytokines and activating MAPKs. Hence, RC seems to have an immune enhancement effect.
Keywords
Rubus coreanus Miquel (RC); nitric oxide (NO); reactive oxygen species (ROS); cyclooxygenase-2 (COX-2); inductible nitric oxide (iNOS); mitogen-activated protein kinase (MAPK);
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