Browse > Article

The Effects of Cuscuta japonica Chois on Gene Expression in RANKL-induced RAW 264.7 Cell  

Kim, Joon-Yeon (Department of preventive medicine, College of Oriental Medicine, Kyungwon University)
Hwang, Gwi-Seo (Department of preventive medicine, College of Oriental Medicine, Kyungwon University)
Publication Information
Journal of Society of Preventive Korean Medicine / v.14, no.2, 2010 , pp. 77-89 More about this Journal
Abstract
Objectives : This study was performed to evaluate the effect of CJ(Cuscuta japonica Chois) on osteoclast differentiation and gene expression. Methods : The osteoclastogenesis and gene expression were determined in RANKL(receptor activator of nuclear factor kappa B ligand)－stimulated RAW 264.7. The results were summarized as followes. Results : CJ decreased the number of TRAP positive cell in RANKL-stimulated RAW264.7 cell. CJ decreased the expression of RANK(receptor activator of nuclear factor kappa B), $TNF{\alpha}$, and IL-6 in RANKL-stimulated RAW264.7 cell. CJ decreased the expression of iNOS and COX-2 in RANKL-stimulated RAW264.7 cell. CJ decreased the expression of Cathepsin K in RANKL-stimulated RAW264.7 cell. Conclusions : It is concluded that CJ might decrease the bone resorption resulted from decrease of osteoclast differentiation and it's related gene expression.
Keywords
Cuscuta japonica Chois(CJ); RANKL; RANK;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Lin TH, Yang RS, Tang CH, Lin CP, Fu WM. PPARgamma inhibits osteogenesis via the down-regulation of the expreCJion of COX-2 and iNOS in rats. Bone. 2007 Oct ; 41(4) : 562-74.   DOI   ScienceOn
2 Zheng H, Yu X, Collin-Osdoby P, Osdoby P. RANKL stimulates inducible nitric-oxide synthase expression and nitric oxide production in developing osteoclasts. An autocrine negative feedback mechanism triggered by RANKL-induced interferonbeta via NF-kappaB that restrains osteoclastogenesis and bone resorption. J Biol Chem. 2006 ; 281(23) : 15809-20.
3 Kim MS, Day CJ, Selinger CI, Magno CL, Stephens SR, Morrison NA. MCP-1-induced human osteoclast-like cells are tartrateresistant acid phosphatase, NFATc1, and calcitonin receptor-positive but require receptor activator of NF-kappaB ligand for bone resorption. J Biol Chem. 2006 ; 281(2) : 1274-85.   DOI
4 Han SY, Lee NK, Kim KH, Jang IW, Yim M, Kim JH, Lee WJ, Lee SY. Transcriptional induction of cyclooxygenase-2 in osteoclast precursors is involved in RANKL-induced osteoclastogenesis. Blood. 2005 ; 106(4) : 1240-5.   DOI   ScienceOn
5 Wei X, Zhang X, Zuscik MJ, Drissi MH, Schwarz EM, O'Keefe RJ. Fibroblasts express RANKL and support osteoclastogenesis in a COX-2-dependent manner after stimulation with titanium particles. J Bone Miner Res. 2005 ; 20(7) : 1136-48.   DOI   ScienceOn
6 Fukushima H, Jimi E, Kajiya H, Motokawa W, Okabe K. Parathyroid-hormone-related protein induces expression of receptor activator of NF-{kappa}B ligand in human periodontal ligament cells via a cAMP/ protein kinase A-independent pathway. J Dent Res. 2005 ; 84(4) : 329-34.   DOI
7 Khosla S. Minireview: the OPG/RANKL/ RANK system. Endocrinology. 2001 ; 142 (12) : 5050-5.   DOI   ScienceOn
8 Yao Z, Li P, Zhang Q, Schwarz EM, Keng P, Arbini A, Boyce BF, Xing L. Tumor necrosis factor-alpha increases circulating osteoclast precursor numbers by promoting their proliferation and differentiation in the bone marrow through up-regulation of c-Fos expression. J Biol Chem. 2006 ; 281(17) : 11846-55.   DOI
9 Liu XH, Kirschenbaum A, Yao S, Levine AC. Interactive effect of interleukin-6 and prostaglandin E2 on osteoclastogenesis via the OPG/RANKL/RANK system. Ann N Y Acad Sci. 2006 ; 1068 : 225-33.   DOI   ScienceOn
10 Cho ES, Yu JH, Kim MS, Yim M. Rolipram, a phosphodiesterase 4 inhibitor, stimulates osteoclast formation by inducing TRANCE expression in mouse calvarial cells. Arch Pharm Res. 2004 ; 27(12) : 1258-62   과학기술학회마을   DOI
11 Lee ZH, Kim HH. Signal transduction by receptor activator of nuclear factor kappa B in osteoclasts. Biochem Biophys Res Commun. 2003; 305(2):211-4.   DOI   ScienceOn
12 Gallea S, Lallemand F, Atfi A, Rawadi G, Ramez V, Spinella-Jaegle S, Kawai S, Faucheu C, Huet L, Baron R, Roman- Roman S. Activation of mitogen-activated protein kinase cascades is involved in regulation of bone morphogenetic protein-2- induced osteoblast differentiation in pluripotent C2C12 cells. Bone. 2001 ; 28(5) : 491-8.   DOI   ScienceOn
13 Oh H, Kang DG, Lee S, Lee HS. Angiotensin converting enzyme inhibitors from Cuscuta japonica Choisy. J Ethnopharmacol. 2002 ; 83(1-2) : 105-8.   DOI
14 Han SY, Lee NK, Kim KH, Jang IW, Yim M, Kim JH, Lee WJ, Lee SY. Transcriptional induction of cyclooxygenase-2 in osteoclast precursors is involved in RANKL-induced osteoclastogenesis. Blood. 2005 ; 106(4):1240-5. Epub 2005 Apr 28.   DOI   ScienceOn
15 John T. Swarthout, Richard C. D'Alonzob, Nagarajan Selvamurugan and Nicola C. Partridge. Parathyroid hormone-dependent signaling pathways regulating genes in bone cells. Gene 2002 ; 282(1-2) : 1-17.   DOI
16 이재복, 이병렬, 兎絲子藥針의 抗癌作用및 免疫效果에 對한 實驗的硏究. 大韓鍼灸學會誌2001 ; 18 : 94-104.
17 Koo BS, Choi EG, Park JB, Cho CH, Chung KH, Kim CH. Neuroprotective effect of Chuk-Me-Sun-Dan on NMDAand AMPA-evoked nitric oxide synthase activity in mouse brain. Immunopharmacol Immunotoxicol. 2005 ; 27(3) : 499-514.   DOI   ScienceOn
18 Ye M, Li Y, Yan Y, Liu H, Ji X Determination of flavonoids in Semen Cuscutae by RP-HPLC. J. Pharm. Biomed. Anal. 2002 ; 28(3-4) : 621-8.   DOI   ScienceOn
19 Yen FL, Wu TH, Li CC, Lin TJ. Hepatoprotective and antioxidant effects of Cuscuta chinensis against acetaminopheninduced hepatotoxicity in rats. J Ethnopharmacol. 2007 ; 111(1) : 123-8.   DOI   ScienceOn
20 Pang M, Martinez AF, Fernandez I, Balkan W, Troen BR. AP-1 stimulates the cathepsin K promoter in RAW 264.7 cells. Gene. 2007;403(1-2):151-8.   DOI   ScienceOn
21 全國韓醫科大學敎授. 本草學, 永林出版社, 서울, 1994, pp568-9.
22 Kumamoto H, Ooya K. Expression of parathyroid hormone-related protein(PTHrP), osteoclast differentiation factor(ODF)/receptor activator of nuclear factor-kappaB ligand (RANKL) and osteoclastogenesis inhibitory factor(OCIF)/osteoprotegerin(OPG) in ameloblastomas. J Oral Pathol Med. 2004 ; 33(1) : 46-52.   DOI   ScienceOn
23 Reddy SV. Regulatory mechanisms operative in osteoclasts. Crit Rev Eukaryot Gene Expr. 2004 ; 14(4) : 255-70.   DOI   ScienceOn
24 Murakami A, Song M, Ohigashi H. Phenethyl isothiocyanate suppresses receptor activator of NF-kappaB ligand (RANKL)-induced osteoclastogenesis by blocking activation of ERK1/2 and p38 MAPK in RAW264.7 macrophages. Biofactors. 2007 ;30(1) : 1-11.   DOI   ScienceOn
25 Boyce BF, Yamashita T, Yao Z, Zhang Q, Li F, Xing L. Roles for NF-kappaB and c-Fos in osteoclasts. J Bone Miner Metab. 2005 ; 23 Suppl : 11-5.   DOI