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http://dx.doi.org/10.5532/KJAFM.2008.10.2.047

Physiological and Biochemical Responses to Ozone Toxicity in Five Species of genus Quercus Seedlings  

Kim, Du-Hyun (Divison of Forest Genetic Resources, Korea Forest Research Institute)
Han, Sim-Hee (Divison of Forest Genetic Resources, Korea Forest Research Institute)
Ku, Ja-Jung (Divison of Forest Genetic Resources, Korea Forest Research Institute)
Lee, Kab-Yeon (Divison of Forest Genetic Resources, Korea Forest Research Institute)
Kim, Pan-Gi (Division of Forest Environment and Resources, Kyungpook National University)
Publication Information
Korean Journal of Agricultural and Forest Meteorology / v.10, no.2, 2008 , pp. 47-57 More about this Journal
Abstract
Physiological and biochemical changes of five species of genus Quercus exposed to ozone fumigation were investigated to assess their tolerance against ozone toxicity. At the end of 150 ppb ozone fumigation, chlorophyll contents, photosynthetic characteristics, malondialdehyde(MDA) and antioxidative enzyme activities were measured in the leaves of five Quercus species(Quercus acutissima, Q. aliena, Q. palustris, Q. serrata, and Q. variabilis). Chlorophyll and carotenoid contents, net photosynthesis and carboxylation efficiency decreased after ozone treatment, indicating that $O_3$-exposed plants underwent physiological inhibition. The reduction rate of total chlorophyll contents and carboxylation efficiency were respectively 15% and 34% for Q. aliena and 38% and 62% for Q. variabilis. The amount of MDA increased with the highest increase rate of 140% in Q. acutissima which also showed the highest increase rate(60%) of superoxide dismutase(SOD). Ascorbate peroxidase(APX) activity increased in Q. variabilis, Q. serrata and Q. acutissima by ozone treatment. Based on our results, ozone tolerance of the five Quercus species was ranked as Q. aliena>Q. palustris>Q. serrata>Q. variabilis>Q. acutissima. We concluded that chlorophyll contents, photosynthesis, MDA content and antioxidative enzymes were the important physiological markers for tolerance against ozone stress, which were closely related with one another.
Keywords
Oak; Chlorophyll content; Photosynthesis; MDA; Antioxidative enzyme;
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