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Effete of Ozone Uptake Rate on Photosynthesis and Antioxidant Activity in the Leaves of Betula Species  

이재천 (임업연구원 임목육종부)
한심희 (임업연구원 임목육종부)
장석성 (임업연구원 임목육종부)
조경진 (임업연구원 임목육종)
김용율 (임업연구원 임목육종부)
Publication Information
Korean Journal of Agricultural and Forest Meteorology / v.4, no.2, 2002 , pp. 72-79 More about this Journal
Abstract
This study was conducted to compare the physiological and biochemical responses of four Betula species in response to ozone, and to find out the relationship between ozone uptake rate and photosynthesis or antioxidant activity. One-year-old seedlings of four Betula sp, B. costata, B. davurica, B. platyphylla var, japonica, and B. ermani, exposed to 100 pub ozone concentration for 8h day$^{-1}$ for 5 weeks in fumigation chamber. Ozone uptake rate, photosynthesis, SOD and GR activity were measured in the leaves of four species once a week. Cumulative ozone uptake rate was largest in the loaves of B. costata(53.8 mmol m$^{-2}$ ), smallest in the leaves of B. davurica(35.5 mmol m$^{-2}$ ). Photosynthesis of four Betula sp. exposed to ozone reduced relative to control, but the photosynthetic responses with changing stomatal conductance were different among species. Ozone exposure increased SOD activities of four species at the early exposing period, but after a critical point SOD activity decreased gradually. GR activity of B. costata was similar to the change of SOD activity, but the others showed the different patterns from B. costata. In conclusion, decreasing both SOD and GR activity at the critical point, B. costata may be sensitive species in response to ozone. In contrast, the others may be resistant species, which gradually increase GR activity following ozone exposure. GR activity was not always in accord with the change of SOD activity against ozone uptake, and the different responses between species were supposed to be affected by the cumulative ozone uptake.
Keywords
ozone uptake; photosynthesis; antioxidant; Betula sp; stomatal conductance; SOD; GR;
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1 박용규, 설일환, 김학윤, 정일경, 신동일, 1998: 단기간 오존처리에 의한 두 잡종포플러의 SOD 활성과 SOD 유전자 발현 변화. 한국육종학회지, 30, 36-41
2 이재천, 김인식, 여진기, 구영본, 2001: 잎의 가시적 피해에 따른 오존에 대한 미류나무(Populus deltoides) 클론간 감수성 비교. 한국임학회지, 90, 10-18.
3 한심희, 2000: 중금속 오염 토양에서 포플러류 수종의 중금속 내성과 모래밭 버섯 균근균 접종에 의한 Cd 내성 변화. 서울대학교박사학위논문, 152 p.
4 Bortier, K., K. Vandermerien, L. De Temmerman and R. Ceulemans, 2001: Growth, photosynthesis and ozone uptake of young beech(Fagus sylvatica L.) in response to different ozone exposures. Trees, 15, 75-82.
5 Chanway, C.P. and V.C. Runeckles, 1984: The role of superoxide dismutase in the susceptibility of bean leaves to ozone injury. Canadian Journal of Botany, 62, 236-240.
6 Lidon, F.C. and F.S. Henriques, 1993: Oxygen metabolism in higher plant chloroplasts. Photosynthetica 29, 249-279.
7 Paakkonen, E., T. Holopainen and L. Kärenlampi, 1997: Differences in growth, leaf senescence and injury, and stomatal density in birch(Betula pendula Roth.) in relation to ambient levels of ozone in Finland. Environmental Pollution, 96, 117-127.
8 Rao, M.V. and K.R. Davis, 2001: The physiology of ozone induced cell death. Planta, 213, 682-690.
9 Lee, J.C., S.H. Han, C.S. Kim, S.S. Jang and P.G. Kim, 2002: Physiological responses and ozone uptake of five Betula sp. exposed to ozone. Proceedings of the 2nd international workshop on advanced flux network and flux evaluation, AsiaFlux, 84-86.
10 Nali, C., L. Guidi, F. Filippi, G.F. Soldatini and G. Lorenzini, 1998: Photosynthesis of two poplar coles contrasting in O3 sensitivity. Trees, 12, 196-200.
11 Paakkonen, E., J. Vahala, T. Holopainen, R. Karjalainen and L. Karenlampi, 1996: Growth responses and related biochemical and ultrastructural changes of the photosynthetic apparatus in birch(Betula pendula) saplings exposed to low concentrations of ozone. Tree Physiology, 16, 597-605.
12 Mortensen, L. and O. Skre, 1990: Effects of low ozone concentrations on growth of Betula pubescens Ehrh., Betula verrucosa Ehrh. and Alnus incana (L.) Moench. New Phytologist, 115, 165-170.
13 Skarby L., E. Troeng and C.A. Boström, 1987: Ozone uptake and effects on transpiration, net photosynthesis, and dark respiration in Scotch pine. Forest Science, 33, 801-808.
14 Tang, Y., B.I. Chevone and J.L. Hess, 1999: Ozone-responsive proteins in a tolerant and sensitive clone of white clover (Trifolium repens). Environmental Pollution, 104, 89-98.   DOI   ScienceOn
15 Sheng, Y., G.K. Podila and D.F. Karnosky, 1997: Differences in $O_3$-induced superoxide dismutase and glutathione antioxidant expression in $O_3$ tolerant and sensitive trembling aspen(Populus tremuloides Michx.) clones. Forest Genetics, 4, 25-33.
16 Shannon, J.G. and C.L. Mulchi, 1974: Ozone damage to wheat varieties at anthesis. Crop Science, 14, 335-337.
17 Heath, R.L., 1980: Initial events in injury to plants by air pollutants. Annual Review of Plant Physiology, 31, 395-431.
18 Asada, K., 1999: The water-water cycle in chloroplasts: Scavenging of active oxygens and dissipation of excess photons. Annual Review of Plant Physiology and Plant Molecular Biology, 50, 601-639.
19 김명희, 이수욱, 1992: 조경수목의 대기오염물질에 대한 방어기능 (I)- SOD 활성을 중심으로-. 한국임학회지, 81, 164-176.
20 Allen, R.D., 1995: Overexpression of chloroplastic Cu/Zn superoxide dismutase in plants. Methods in Molecular Biology, Vol.44: Agrobacterium Protocols., K.M.A. Gartland and M.R. Davey. (Eds) Humana Press Inc., 309-323.
21 Laisk, A., O. Kull and H. Moldau, 1989: Ozone concentration in leaf intercellular air species is close to zero. Plant Physiology, 90, 1163-1167.
22 Pitcher, L.H., E. Brennan, A. Hurley, P. Dunsmuir, J.M. Tepperman and B.A. Zilinskas, 1991: Overproduction of petunia chloroplastic copper/zinc superoxide dismutase does not confer ozone tolerance in transgenic tobacco. Plant Physiology, 97, 452-455.
23 Sheng, W.S., B.I. Chevone and J.L. Hess, 1993: Photosynthetic inhibition and superoxide dismutase activity in soybean cultivars exposed to short-term ozone fumigations. Environmental Pollution, 80, 45-52.
24 Beauchamp C. and I. Fridovichi, 1971: Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44, 276-287.
25 Oksanen, E., G. Amores, H. Kokko, J. M. Santamaria and L. Karenlampi, 2001: Genotypic variation in growth and physiological responses of Finish hybrid aspen(Populus tremuloides P. tremula) to elevated tropospheric ozone concentration. Tree Physiology, 21, 1171-1181.
26 Sharma, Y.K. and K.R. Davis, 1994: Ozone-induced expression of stress-related genes in Arabidopsis thalina. Plant Physiology, 105, 1089-1096.
27 Asada K, M. Takahashi and M. Nagate, 1974: Assay and inhibitors of spinach superoxide dismutase. Agricultural and Biological Chemistry, 38, 471-473.
28 Evans, L.S., J.H. Adamski and J.R. Renfro, 1996: Relationship between cellular injury, visible injury of leaves, and ozone exposure levels for several dicotyledonous plant species a great smoky mountains national park. Environmental and Experimental Botany, 36, 229-237.
29 Smith, I.K., T.L. Vierheller and C.A. Thorne, 1988: Assay of glutathione reductase in crude tissue homogenates using 5,5'-dithibis(2-nitrobenzoic acid). Analytical Biochemistry, 175, 408-413.
30 Coleman, M.D., J.G. Isebrands, R.E. Dickson and D.F. Kamosky, 1995: Photosynthetic productivity of aspen clones varying in sensitivity to tropospheric ozone. Tree Physiology, 15, 585-592.
31 Lee, J.C., S.H. Han, S.S. Jang and Y.P. Hong, 2001: Ozone uptake and tolerance test of five tree species exposed to ozone. Plant Cell Biotechnology for Molecular Breeding. 163.
32 Tepperman, J.M. and P. Dunsmuir, 1990: Transformed plants with elevated levels of chloroplastic SOD are not more resistant to superoxide toxicity. Plant Molecular Biology, 14, 501-511.
33 Sen Gupta, A., R.G. Alscher and D. McCune, 1991: Response of photosynthesis and cellular antioxidnats to ozone in Populus leaves. Plant Physiology, 96, 650-655.
34 Bennet, J.H., E.H. Lee and H.E. Heggestad, 1984: Biochemical aspect of plant. Gaseous Air Pollutants and Plant Metabolism, M.J. Koziol and F.R. Whatley (Eds.) Butterworth England, 413-424.