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http://dx.doi.org/10.7585/kjps.2015.19.1.5

Development of an Official Analytical Method for Determination of Imazapyr in Agricultural Commodities using HPLC-UVD  

Jang, Jin (Pesticide and Veterinary Drug Residues Division, National Institute of Food and Drug Safety Evaluation, Department of Food Safety Evaluation, Ministry of Food and Drug Safety)
Kim, Heejung (Pesticide and Veterinary Drug Residues Division, National Institute of Food and Drug Safety Evaluation, Department of Food Safety Evaluation, Ministry of Food and Drug Safety)
Ko, Ah-Young (Pesticide and Veterinary Drug Residues Division, National Institute of Food and Drug Safety Evaluation, Department of Food Safety Evaluation, Ministry of Food and Drug Safety)
Lee, Eun-Hyang (Pesticide and Veterinary Drug Residues Division, National Institute of Food and Drug Safety Evaluation, Department of Food Safety Evaluation, Ministry of Food and Drug Safety)
Joo, Yoon Ji (Pesticide and Veterinary Drug Residues Division, National Institute of Food and Drug Safety Evaluation, Department of Food Safety Evaluation, Ministry of Food and Drug Safety)
Kim, Jinhong (Hazardous Substances Analysis Division, Center for Food and Drug Analysis, Daejeon regional Korea Food and Drug Administration)
Chang, Moon-Ik (Pesticide and Veterinary Drug Residues Division, National Institute of Food and Drug Safety Evaluation, Department of Food Safety Evaluation, Ministry of Food and Drug Safety)
Rhee, Gyu-Seek (Pesticide and Veterinary Drug Residues Division, National Institute of Food and Drug Safety Evaluation, Department of Food Safety Evaluation, Ministry of Food and Drug Safety)
Publication Information
The Korean Journal of Pesticide Science / v.19, no.1, 2015 , pp. 5-13 More about this Journal
Abstract
A chromatographic method for the determination of imazapyr, a non-selective herbicide, in agricultural commodities was developed to use safety control of pesticide residue on crops, and was fully validated as an official method for residue analysis. Agricultural commodities, mandarin (fruit), hulled rice (cereal grains), pepper (vegetables), potato (potatoes) and soybean (beans) were extracted with methanol and partitioned with dichloromethane to remove the interference obtained from sample extracts, adjusting pH to 2.5 by 4N hydrochloric acid. Finally, they were analyzed by high performance liquid chromatography coupled to UV detector (HPLC-UVD). The developed method had the linearity in the range of test concentrations with coefficients of determination ($r^2$) more than 0.99. Recovery studies were carried out at three concentration levels (LOQ, 10LOQ, and 50LOQ) performing five replicates at each level. Recoveries were ranged between 72.1 to 108.0%, with relative standard deviations less than 10%. A consistent recovery was determined according to the CODEX guidelines (CAC/GL40, 2003). Finally, LC/MS with selected ion monitoring was also applied to confirm the suspected residues of imazapyr in agricultural samples. This developed method for determination of imazapyr residues in agricultural commodities. can be used as an official method.
Keywords
residue analysis; imazapyr; HPLC-UVD; LC/MS; agricultural commodities;
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