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http://dx.doi.org/10.7585/kjps.2014.18.2.69

Development of Analytical Method for Cymoxanil in Agricultural Commodities using HPLC/UVD  

Kim, Ji-Yoon (Department of Biological Environment, College of AgFriculture and Life Sciences, Kangwon National University)
Kim, Hea-Na (Department of Biological Environment, College of AgFriculture and Life Sciences, Kangwon National University)
Kim, Ja-Young (Croen Research Incorporated)
Kim, Jong Geol (Environment Friendly Agricultural Products Safety Center)
Ham, Hun-Ju (Environment Friendly Agricultural Products Safety Center)
Lee, Young-Deuk (Division of Life and Environmental Science, Daegu University)
Hur, Jang-Hyun (Department of Biological Environment, College of AgFriculture and Life Sciences, Kangwon National University)
Publication Information
The Korean Journal of Pesticide Science / v.18, no.2, 2014 , pp. 69-78 More about this Journal
Abstract
In the present study, we developed an official individual analytical method for cymoxanil using HPLC/UVD, respectively in different representative crops. Individual analytical methods for these pesticides are not included in the Korea food code. The samples were extracted with acetonitrile, concentrated and partitioned with dichloromethane and saturated sodium chloride solution. For cymoxanil, extracts were concentrated and clean-up through silica gel column chromatography with dicloromethane/acetone (60/40 v/v) and subjected to instrumental analysis. The limit of detection (LOD) for cymoxanil were 0.1 ng and 1 ng respectively and limit of quantitation (LOQ) were 0.02 mg/kg. Recoveries for cymoxail ranged from 79.6~107.6% respectively, at fortification level of 0.02 mg/kg (LOQ), 0.2 mg/kg (10 LOQ) and 1.0 mg/kg (50 LOQ) and the coefficient of variation (CV) was less than 10%, regardless of sample types. These results were further confirmed with LC/MS. The proposed simultaneous analysis method is reproducible and sensitive enough to determine the residues of cymoxanil in the agricultural commodities. According to the validation data and performance characteristics and high sample throughput, the proposed method is suitable for routine application.
Keywords
analytical method; Cymoxanil; HPLC/UVD; LC/MS;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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