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http://dx.doi.org/10.7585/kjps.2013.17.4.468

Genome Sequence Analysis of Chrysanthemum White Rust pathogen Puccinia horiana and Sterol 14-demethylase as Drug Target  

Kim, Jeong-Gu (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
Park, Sang Kun (Floriculture Research Division, National Institute of Horticultural & Herbal Science)
Park, Ha-Seung (Yesan Chrysanthemum Experiment Station, Chungcheongnam-do Agricultural Research and Extension Services)
Kwon, Soo-Jin (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
Kim, Seung Hwan (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
Lee, Dong-Jun (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
Sohn, Seong-Han (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
Lee, Byoung Moo (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
Bae, Shin-Chul (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
Ahn, Il-Pyung (Department of Agricultural Biotechnology, National Academy of Agricultural Science, Rural Development Administration)
Kim, Changhoon (Macrogen Inc.)
Baek, Jeong Hun (Macrogen Inc.)
Publication Information
The Korean Journal of Pesticide Science / v.17, no.4, 2013 , pp. 468-472 More about this Journal
Abstract
Chrysanthemum is an economically important horticultural plant in many countries. The white rust is one of the most devastating diseases caused by an obligate fungal pathogen Puccinia horiana. This is being controlled mostly by application of chemicals. In Korea, 26 items are registered and 10 items contain 6 triazole compounds. To identify and to obtain the information of the drug target for triazoles, possible sterol 14-demethylase orthologues were extracted. From the draft genome information, the nucleotide sequence of the sterol 14-demethylase gene was identified. The amino acid sequence was deduced and the tertiary structure of the enzyme was predicted. This protein showed no less than 84% amino acid sequence identities to those of genus Puccinia and no more than 68% to those of other genus.
Keywords
Puccinia horiana; Chrysanthemum white rust; genome; drug target; sterol 14-demethylase;
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