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http://dx.doi.org/10.7585/kjps.2012.16.4.357

Isolation and Characterization of Bacillus Species Possessing Antifungal Activity against Ginseng Root Rot Pathogens  

Kim, Byung-Yong (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
Ahn, Jae-Hyung (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
Weon, Hang-Yeon (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
Song, Jaekyeong (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
Kim, Sung-Il (Institute of Ginseng and Medicinal Plants Research, Gangwon Agricutlural Research and Extension Services)
Kim, Wan-Gyu (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
Publication Information
The Korean Journal of Pesticide Science / v.16, no.4, 2012 , pp. 357-363 More about this Journal
Abstract
Ginseng (Panax ginseng C. A. Meyer) is an economically important crop in Korea. While the consumption of the crop is gradually increasing, the yield is decreasing due to the injury of continuous cultivation or infection of soil-borne fungal pathogens such as Cylindrocarpon destructans, Fusarium solani, Rhizoctonia solani and Sclerotinia nivalis. In order to find promising biocontrol agents, we have isolated 439 soil bacteria from ginseng cultivated soil and tested their antifungal activities against ginseng rot pathogens. Among them, 3 strains were finally selected and tested for the elucidation of their genetic and biochemical properties. They were identified as Bacillus amyloliquefaciens using phylogenetic analysis based on 16S rRNA gene sequences. Moreover, all selected strains showed positive reaction for PCR detection targeting biosynthetic gene sequences of iturin A and surfactin. The results provided promising evidences that the bacterial strains isolated from ginseng cultivated soil can be novel biocontrol agents for ginseng cultivaion.
Keywords
Antifungal activity; Bacillus; Ginseng rot;
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