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http://dx.doi.org/10.7585/kjps.2012.16.2.187

Evaluation of endocrine disrupting effect of the herbicide Alachlor on Japanese Medaka using short term reproduction assay  

Lee, Je-Bong (National Academy of Agricultural Science)
Park, Yoen-Ki (National Academy of Agricultural Science)
Choi, Young-Woong (National Academy of Agricultural Science)
Kim, Byung-Seok (National Academy of Agricultural Science)
Kwon, Hye-Young (National Academy of Agricultural Science)
Jin, Yong-Duk (National Academy of Agricultural Science)
Im, Geon-Jae (National Academy of Agricultural Science)
Kang, Kyu-Young (Dept. of Environ. Biotechnology, Gyeong Sang National University)
Publication Information
The Korean Journal of Pesticide Science / v.16, no.2, 2012 , pp. 187-193 More about this Journal
Abstract
Acute toxicity, water resolvability and short term reproduction test on Japanese medaka (Oriyzias latipes) for evaluating alachlor susceptibility to endocrine system were studied. Alachlor is known for suspected endocrine distruptors. As the results of tests, $LC_{50}$ (Median lethal concentration) was determined as 2.36 (1.994~2.805) mg/L, and test water replaced at 7 day intervals as its water resolvability was less than 20% in 7 days. The short term reproduction tests on Japanese medaka (Oriyzias latipes) were performed with a solvent control group, a treated group (alachlor concentrations of 0.02, 0.04, 0.11, 0.27, 0.68 ppm) and a positive control group (17 ${\beta}$ estradiol, 0.01, 0.1, 0.5 ppb). The number of spawning and embryo rates were declined in a alachlor-dose dependent manner, and the number of unfertilized eggs rates were in contrast increased depending on the concentrations. Further study should be needed to confirm whether the adverse effects may be effected by the concentrations. Additionally, alachlor was evaluated as a non-vitellogenin by the result of a test of significance of the vitellogenin content test for determination of the effect of estrogen among the endocrine disruptors.
Keywords
Alachlor; short term reproduction test; vitellogenin;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 US/EPA (1998) Final report of endocrine disruptor screening and testing advisory committee.
2 US/EPA (2009) Endocrine disruptor screening program test guidelines.
3 Vryzas Z., C. Alexoudis, G. Vassiliou, K. Galanis, and E. Papadopoulou-Mourkidou (2011) Determination and aquatic risk assessment of pesticide residue in riparian drainage canals in Northeastern Greece. Ecotoxicol Environ Saf. 74(2):174-181.   DOI   ScienceOn
4 National Institute of Environmental Research (1999) Comprehension and Reaction of Endocrine Disrupting Chemicals.
5 Lee, J. B., J. S. Shin, H. D. Lee, M. Jeong, A. You and K. Y. Kang (2005) Risk Assessment for Estrogenic Effect of the Suspected Endocrine Disrupting Pesticides, The Korea Journal of Pesticide Science Vol. 8(2):95-102.   과학기술학회마을
6 Lee, C., H. M. Kim, J. Yoon, S. H. Song, J. Ryu, E. K Kim, C. Y. Yang, Y. H. Chung, K. Choi and M. S. Lee (2007) Ecological Risk Assessment of Alachlor Using Medaka (Oryzias latipes) and Earthworm(Eisenia fetida). Korean J. Environ. Biol 25(1):1-7.   과학기술학회마을
7 Korea Crop Protection Association (2011) Agrochemicals Use Guide Book.
8 Boenke, A., C. Searle and T. Karjalainen (2002) Contribution of European research to endocrine disruptors. analytica Chimica Acta 473:161-165.   DOI
9 Lee, C., J. Ryu, S. Y. Park, K. Choi, S. H. Jeon, J. G. Na and D. G. Rhee (2004) Effect of alachlor on the early development and induction of estrogen-responsive genes. Organohalogen compounds 66:2951-2957.
10 Kang I. K., Y. Hirofumi, O. Yuji, T. Yukinari, Y. Tomomi, M. Masanobu, I. Nobuyoshi, T. Hiroshi, and H. Tsuneo (2002) Effect of 17 ${\beta}$-estradiol on the reproduction of Japanese medaka(Oryzias latipes). Chemosphere , 47:71-80.   DOI   ScienceOn
11 Lange A., Y. Katsu, S. Miyagawa, Y. Ogino, H. Urushitani, T. Kobayashi, T. Hirai, J. A. Shears, M. Nagae, J. Yamamoto, Y. Ohnishi, T. Oka, N. Tatarazako, Y. Ohta, C. R. Tyler, and T. Iguchi (2011) Comparative responsiveness to natural and synthetic estrogens of fish species commonly used in the laboratory and field monitoring. Aquat Toxicol. 2011 Sep 28.
12 Patyna, P. J., R. A. Davi, T. F. Parketon, R. P. Brown and K. R. Cooper (1999) A proposed multigenerational protocol for Japanese Medaka(Oryzias latipes) to evaluate effect of endocrine disruptors. Sci. Total Environ. 233, 211-220.   DOI
13 Sanchez-Camazona M., L. F. Lorenzo, and M. J. Sanchez-Martin (2005) Atrazine and alachlor inputs to surface and ground waters in irrigated corn cultivation areas of Castilla-Leon, Spain. Environ monit Assess. 105(1-3):11-24.   DOI
14 Shosaku K., I. Hidenori, M. Nobukazu, O. Yuta, and M. Yasumoto (2002) Fish test for endocrine-disruption and estimation of water quality of Japanese rivers. Water research 36:2161-2166.   DOI   ScienceOn
15 Tomlin, C. D, S. (2009) Alachlor, pp. 23-24, in The Pesticide Manual., UK.
16 Tsutomu S. and W. Meiko (2000) Effect of certain chemicals on the reproduction of medaka(Oryzias latipes). Chemosphere 40:239-243.   DOI   ScienceOn
17 US/EPA (1997) EPA special report on endocrine disruption.
18 Aschberger, K., P. Castello, E. Hoekstra, S. Karakitsios, S. Munn, S. Pakalin and D. Sarigianis (2010) Bisphenol A and baby bottles: challengers and perspectives. JRC scientific and technical reports.