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http://dx.doi.org/10.7585/kjps.2012.16.2.178

The isolation of Bacillus subtilis KYS-10 with antifungal activity against plant pathogens  

Kang, Dae-Won (College of Life Science and Natural Resources, Wonkwang University)
Ryu, Il-Hwan (College of Life Science and Natural Resources, Wonkwang University)
Han, Seong-Soo (College of Life Science and Natural Resources, Wonkwang University)
Publication Information
The Korean Journal of Pesticide Science / v.16, no.2, 2012 , pp. 178-186 More about this Journal
Abstract
This study was investigated for the purpose of the isolation and identification of antagonistic bacteria with antifungal activity against plant pathogens. This bacteria denominated Bacillus subtilis KYS-10 and the optimum growth condition were 4% sucrose, 1% yeast extract, 0.2% $K_2HPO_4$, pH 7, 150 rpm, $30^{\circ}C$, 8 day. The antifungal activities against nine plant pathogens determined inhibition zone size by diffusion methods. The results, G. zeae (scab) 70 mm and P. grisea KACC 40439 (blast), P. capsici KACC 40177 (phytophthora blight) and C. destructans KACC 41077 (root rot of ginseng) 40~43 mm, and C. gloeosporioides KACC 43520 (ripe rot), C. gloesporioides KACC 40003 (anthracnose), S. shiraiana KACC 41065 (stem rot) and S. shiraiana (mulberry sclerotial disease) 35~39 mm and F. Oxysporum KACC 44452 (bulb rot of ginseng) 28 mm. From these experiment results, author suggest that Bacillus subtilis KYS-10 would be developed as a biological control agent thorough the field experimet in the near future.
Keywords
Bacillus subtilis KYS-10; Isolation and identification; Optimum growth condition; Plant pathogenes; Antifungal activity;
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