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http://dx.doi.org/10.7585/kjps.2012.16.1.069

Effects on Biomarkers and Endocrine in Muddy Loach (Misgurnus anguillicaudatus) under 21 day Exposure to Methomyl  

Han, Sun-Young (Environmental Toxicology Research Center, Korea Institute of Toxicology)
Kim, Ja-Hyun (Environmental Toxicology Research Center, Korea Institute of Toxicology)
Gwon, Ga-Young (Environmental Toxicology Research Center, Korea Institute of Toxicology)
Yeom, Dong-Hyuk (Environmental Toxicology Research Center, Korea Institute of Toxicology)
Publication Information
The Korean Journal of Pesticide Science / v.16, no.1, 2012 , pp. 69-77 More about this Journal
Abstract
To evaluate the effect of endocrine disruption chemicals (EDCs) to aquatic organisms, muddy loach (Misgurnus anguillicaudatus) was exposed to low concentration methomyl for 21 days in order to identify the effect of biomarkers and endocrine. Vitellogenin (VTG) in blood plasma, which used widely as validated biomarker for endocrine disruption, was significantly greater in male fish exposed to 0.4 mg/L and 2 mg/L methomyl, and in female fish exposed to 0.08 mg/L, 0.4 mg/L, and 2 mg/L methomyl for 21 days (p<0.05). This results suggest that methomyl have probability of endocrine disruption to organism on aquatic system. While inhibition of Acetylcholinesterase (AChE) activity and increase of DNA damage in comet assay were verified by fish exposed to methomyl, change of ethoxyresorufin-O-deethylase (EROD) activity was not occurred, comparing the control group (p<0.05). Indicators at the level of organism such as condition factor (CF), hepato-somatic index (HSI), and gonado-somatic index (GSI) were not influenced by exposure of methomyl. In conclusion, these results showed the possibility of methomyl in regard to not only endocrine disruption but also impacts on biochemical biomarkers to aquatic organisms.
Keywords
Methomyl; Misgurnus anguillicaudatus; Vitellogenin; Biochemical biomarkers; Endocrine disruption;
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