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Development of Analytical Method for Fenoxycarb, Pyriproxyfen and Methoprene Residues in Agricultural Commodities Using HPLC-UVD/MS  

Lee, Su-Jin (Dept. of Herbal Medicine Resource, Kangwon National University)
Kim, Young-Hak (Dept. of Herbal Medicine Resource, Kangwon National University)
Song, Lee-Seul (Dept. of Herbal Medicine Resource, Kangwon National University)
Hwang, Yong-Sun (Dept. of Herbal Medicine Resource, Kangwon National University)
Lim, Jung-Dae (Dept. of Herbal Medicine Resource, Kangwon National University)
Sohn, Eun-Hwa (Dept. of Herbal Medicine Resource, Kangwon National University)
Im, Moo-Hyeog (Food Standard Division, Korea Food and Drug Administration)
Do, Jung-Ah (Food Chemical Residues Division, National Institution of Food and Drug Safety Evaluation)
Oh, Jae-Ho (Food Chemical Residues Division, National Institution of Food and Drug Safety Evaluation)
Kwon, Ki-Sung (Food Chemical Residues Division, National Institution of Food and Drug Safety Evaluation)
Lee, Joong-Keun (Korea Health Industry Development Institute)
Lee, Young-Deuk (Division of Life and Environmental Science, Daegu University)
Choung, Myoung-Gun (Dept. of Herbal Medicine Resource, Kangwon National University)
Publication Information
The Korean Journal of Pesticide Science / v.15, no.3, 2011 , pp. 254-268 More about this Journal
Abstract
Fenoxycarb, pyriproxyfen and methoprene are juvenile hormone mimic insecticide. These insecticides have been widely used for mosquito, fly, scale insects, and Lepidoptera. The purpose of this study was to develop a simultaneous determination procedure of fenoxycarb, pyriproxyfen and methoprene residues in crops using HPLC-UVD/MS. These insecticide residues were extracted with acetone from representative samples of four raw products which comprised brown rice, apple, green pepper, and Chinese cabbage. The extract was diluted with saline water, and then n-hexane/dichloromethane partition was followed to recover these insecticides from the aqueous phase. Florisil column chromatography was additionally employed for final clean up of the extract. The analytes were quantitated by HPLC-UVD/MS, using a $C_{18}$ column. The crops were fortified with each insecticide at 3 levels per crop. Mean recovery ratios were ranged from 80.0 to 104.3% in four representative agricultural commodities. The coefficients of variation were less than 4.8%. Quantitative limit of fenoxycarb, pyriproxyfen, and methoprene was 0.04 mg/kg in crop samples. A HPLC-UVD/MS with selected-ion monitoring was also provided to confirm the suspected residues. The proposed simultaneous analysis method was reproducible and sensitive enough to determine the residues of fenoxycarb, pyriproxyfen and methoprene in the agricultural commodities.
Keywords
fenoxycarb; pyriproxyfen; methoprene; HPLC-UVD/MS; residue; agricultural commodity;
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Times Cited By KSCI : 2  (Citation Analysis)
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