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Analytical Method of Bentazone Residue in Agricultural Commodities Using HPLC-UVD/MS  

Kim, Young-Hak (Dept. of Herbal Medicine Resource, Kangwon National University)
Lee, Su-Jin (Dept. of Herbal Medicine Resource, Kangwon National University)
Song, Lee-Seul (Dept. of Herbal Medicine Resource, Kangwon National University)
Hwang, Young-Sun (Dept. of Herbal Medicine Resource, Kangwon National University)
Lee, Young-Deuk (Division of Life and Environmental Science, Daegu University)
Choung, Myoung-Gun (Dept. of Herbal Medicine Resource, Kangwon National University)
Publication Information
The Korean Journal of Pesticide Science / v.15, no.2, 2011 , pp. 149-159 More about this Journal
Abstract
Bentazone is benzothiadiazole group herbicide, and used to foliage treatment. This herbicide have already been widely used for cereals and vegetables planting in worldwide. This experiment was conducted to establish a determination method for bentazone residue in crops using HPLC-UVD/MS. Bentazone residue was extracted with acetone (adjusted pH 1 with phosphoric acid) from representative samples of five raw products which comprised hulled rice, soybean, apple, green pepper, and Chinese cabbage. The extract was diluted with saline water, and dichloromethane partition was followed to recover bentazone from the aqueous phase. Florisil column chromatography was additionally employed for final clean up of the extract. The bentazone was quantitated by HPLC with UVD, using a YMC ODS AM 303 ($4.6{\times}250$ mm) column. The crops were fortified with bentazone at 3 levels per crop. Mean recovery ratio were ranged from 82.0% for a 0.2 mg/kg in apple to 97.9% for a 0.02 mg/kg in Chinese cabbage. The coefficients of variation were ranged from 0.5% for a 0.02 mg/kg in soybean to 9.7% for a 0.02 mg/kg in Chinese cabbage. Quantitative limit of bentazone was 0.02 mg/kg in representative five crop samples. A LC/MS with selected-ion monitoring was also provided to confirm the suspected residue. Therefore, this analytical method was reproducible and sensitive enough to determine the residue of bentazone in agricultural commodities.
Keywords
bentazone; HPLC-UVD/MS; crop analysis; residue analysis;
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Times Cited By KSCI : 6  (Citation Analysis)
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