Browse > Article

Determination of Ametryn Residue in Agricultural Commodities Using HPLC-UVD/MS  

Lee, Su-Jin (Dept of Herbal Medicine Resource, Kangwon National University)
Kim, Young-Hak (Dept of Herbal Medicine Resource, Kangwon National University)
Song, Lee-Seul (Dept of Herbal Medicine Resource, Kangwon National University)
Choung, Myoung-Gun (Dept of Herbal Medicine Resource, Kangwon National University)
Publication Information
The Korean Journal of Pesticide Science / v.15, no.2, 2011 , pp. 125-133 More about this Journal
Abstract
Ametryn is used in USA, China, and Japan, but not introduced in Korea yet. So, MRL (Maximum Residue Level), and analytical method of ametryn were not establishment in Korea. Therefore, this experiment was conducted to establish a determination method for ametryn residue in crops using HPLC-UVD/MS. Ametryn residue was extracted with acetone from representative samples of five raw products which comprised hulled rice, soybean, apple, green pepper, and Chinese cabbage. The extract was diluted with saline water, and dichloromethane partition was followed to recover ametryn from the aqueous phase. Florisil column chromatography was additionally employed for final clean up of the extract. The ametryn was quantitated by HPLC with UVD, using a Tosoh ODS 120T ($4.6{\times}250$ mm) column. The crops were fortified with ametryn at 2 levels per crop. Mean recovery ratio were ranged from 83.7% for a 0.2 mg/kg in soybean to 91.1% for a 1.0 mg/kg in hulled rice. The coefficients of variation were ranged from 1.2% for a 1.0 mg/kg in hulled rice to 3.6% for a 1.0 mg/kg in soybean. Quantitative limit of amatryn was 0.02 mg/kg in representative 5 crop samples. A LC/MS with selected-ion monitoring was also provided to confirm the suspected residue. Therefore, this analytical method was reproducible and sensitive enough to determine the residue of ametryne in agricultural commodities.
Keywords
Amaetryn; HPLC-UVD/MS; Crop analysis; Residue;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Kwon, C. H., M. I. Chang, M. H. Im, H. Choi, D. I. Jung, S. C. Lee, J. Y. Yu, Y. D. Lee, J. O. Lee, and M. K. Hong (2008) Determination of mandipropamid residues in agricultural commodities using high-performance liquid chromatography with mass spectrometry. Analytical Sci. & Technology 21(6):518-525.
2 John, R. D., G. Wade, and I. J. Barnabas (1996) Determination of triazine herbicides in environmental samples. Journal of Chromatography A. 733:295-335   DOI
3 Glaucia, M. F., I. C. Pinto, and S. F. Jardim (2000A) Mobile phase optimization for the seperation of some herbicide samples using HPLC. Journal of Liquid Chromatography & Related Technologies 23(9):1353-1363   DOI   ScienceOn
4 Glaucia, M. F., I. C. Pinto, and S. F. Jardim (2000B) Use of solid-phase extraction and high-performance liquid chromatography for the determination of triazine residues in water: validation of the method. Journal of Chromatography A 869:463-469   DOI
5 Codex Alimentarius Commission (2003) Guidelines on good laboratory practice in residue analysis, CAC/GL 40-1993, Rev.1-2003, Rome, Italy.
6 International Uniform Chemical Information Database Dataset (IUCLID) (2000) European Commission European Chemicals Bureau.
7 Dale, L. S., L. J. Krutz, W. B. Henry, B. D. Hanson, M. D. Poteet, and C. R. Rainbolt (2010) Sugarcane soils exhibit enhanced atrazine degradation and cross adaptation to other s-triazines, Journal American Society of Sugar Cane Technologists 30:1-10
8 Farre, M. J. Fernandez, M. Paez, L. Granada, L. Barba, H. M. Gutierrez, C. Pulgrarin, and D. Barcelo (2002) Analysis and toxicity of methomyl and ametryn after biodegradation. Anal. Bioanal. Chem. 373:704-709   DOI   ScienceOn
9 Fong, W. G., H. A. Moye, J. N. Seiber, and J. P. Toth (1999) Pesticide residues in food; Methods, technologies, and regulations. Wiley Interscience. pp. 3-4, 40-44, Canada.
10 AOAC (2000) 'Pesticide and industrial chemical residues, In Official method of analysis', 17th ed., pp. 1-88, AOAC International, Arlington, VA, USA.
11 Lee, S. J., Y. S. Hwang, Y. H. Kim, M. Y. Nam, S. B. Hong, W. K. Yun, C. H. Kwon, J. A. Do, M. H. Im, Y. D. Lee, and M. G. Choung (2010) Determination of fomesafen residue in agricultural commodities using HPLC-UVD/MS. Korean J. of Pesticide Sci. 14(2):100-108
12 이영득 (2011) 식품공전 잔류농약분석법 실무 해설서, 식품의약품안전청.
13 장미라, 문현경, 김태랑, 육동현, 김정현, 박석기 (2010) 서울지역 유통 채소류 섭취에 따른 잔류 농약의 위해성 평가. 한국영양학회지 43(4):404-412.
14 Miller, J. M. (2005) Chromatography : concepts and contrasts (2nd), Wiley Intersciense, p. 286-287, USA.
15 Tadeo, J. L., C. S. Brunete, A. I. Garcia-Valcarcel, L. Martinez, and R. A. Perez (1996) Determination of cereal herbicide residue in environmental samples by gas chromatography. Journal of Chromatography A 754:347-365.   DOI
16 US FDA (1999) 'Pesticide Analytical Manual, Vol 1: Multi residue Methods (3rd ed.), US Food and Drug Administration, USA.
17 식품의약품안전청 (2009) 식품공전.
18 Lee, J., H. Park, Y. Keum, C. Kwon, Y. Lee, and J. Kim (2008) Dissipation pattern of boscalid in cucumber under green house condition. Korean Journal of Pesticide Science 12:67-73.
19 Lowell, A. Kleper (1979) Effects of certain herbicides and their combinations of nitrate and nitrite reduction. Plant Physiology 64:273-275.   DOI   ScienceOn
20 Lucio, F. C. M., C. H. Collins, and I. C. S. F. Jardim (2004) New materials for solid-phase extraction and multiclass high-performance liquid chromatographic analysis of pesticides in grapes. Journal of Chromatography A 1032:51-58.   DOI