Browse > Article

Establishment of Analytical Method for Cyprodinil Residue In Apple, Mandarin, Korean Cabbage and Green Pepper  

Lee, Hye-Ri (Agricultural Biotechnology, Seoul National University)
Riu, Myoung-Joo (Agricultural Biotechnology, Seoul National University)
Kim, Eun-Hye (Agricultural Biotechnology, Seoul National University)
Moon, Joon-Kwan (Agricultural Biotechnology, Seoul National University)
Do, Jung-A (National Institute of Food and Drug Evaluation)
Oh, Jae-Ho (National Institute of Food and Drug Evaluation)
Kwon, Ki-Sung (National Institute of Food and Drug Evaluation)
Im, Moo-Hyeog (Korea Food and Drug Administration)
Lee, Young-Deuk (Division of Life and Environmental Science, Daegu University)
Kim, Jeong-Han (Agricultural Biotechnology, Seoul National University)
Publication Information
The Korean Journal of Pesticide Science / v.14, no.4, 2010 , pp. 371-380 More about this Journal
Abstract
This study was performed to develop a precise single residue analytical method of fungicide cyprodinil in representative crops for using as general residue analytical methods which could be applied to most of crops. Apple, mandarin, Korean cabbage and green pepper were selected as representative crops, and they were macerated, extracted with acetonitrile, concentrated and partitioned with n-hexane. Then the extracts were concentrated and cleaned-up through silica gel column with ethyl acetate:n-hexane (15:85, v/v) before concentration and analysis with HPLC. LOQ (limit of quantitation) of cyprodinil was 5 ng (S/N>10) and MQL (method qnantitation limit) was 0.05 mg/kg. Recoveries were measured at three fortification levels (MQL, 10MQL and 100MQL) on crop samples and ranged from 82.0 to 108.2% and coefficients of variation were less than 10% regardless of sample type.
Keywords
Cyprodinil; HPLC; LOQ; MQL; Recovery;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 이종화, 박희원, 금영수, 권찬혁, 이영득, 김정한 (2008) 시설 내 오이재배 중 살균제 Boscalid의 잔류특성. 농약과학회지 12(1):67-73.   과학기술학회마을
2 (주)랩프런티어 (2004) 식품 중 동시다성분 분석법 개선 연구 pp. 89, 114 식품의약품안전청.
3 한국작물보호협회 (2010) 농약사용지침서, 삼정인쇄공사 pp. 144-145.
4 Liu, C., S. Wang, L. Li, H. Zhao, S. Jiang and F. Liu (2010) Analysis of cyprodinil in leek and pepper and its decline under field condition. Environ. Monit. Assess. DOI: 10.1007/s10661-010-1730-y
5 Marin A., J. Oliva, C. Garcia, S. Navarro and A. Barba (2003) Dissipation rates of cyprodinil and fludioxonil in lettuce and table grape in the field and under cold storage conditions. J. Agric. Food Chem. 51:4708-4711.   DOI   ScienceOn
6 McNair, H. M. and J. M. Miller (1997) Basic Gas Chromatography. pp.7, 51, Wiley Interscience, U.S.A.
7 Miller, J. M. (2005) Chromatography : concepts and contrasts (2nd), Wiley Intersciense, pp. 286-287, U.S.A.
8 Moon J. K., H. J. Lee, Y. W. Kim, Y. H. Lee and J. H. Kim (2003) Establishment of pre-harvest interval for cyprodinil in grape. Agric. Chem. Biotechnol. 46(4):174-176.
9 Pose-Juan E., B. Cancho-Grande, R. Rial-Otero and J. Simal- Gandara (2006) The dissipation rates of cyprodinil, fludioxonil, procymidone and vinclozoline during storage of grape juice. Food Control 17:1012-1017.   DOI   ScienceOn
10 Rood, D. (2007) The Troubleshooting and Maintenance Guide for Gas Chromatographyers pp. 15-17, p. 22, Wiley-VCH, U.S.A.
11 Tomlin, C. D. S. (2006) The Pesticide Manual (14th), pp. 264-266, British Crop Production Council, UK.
12 식품의약품안전청 (2010a) 식품의 농약 잔류허용기준.
13 Vaquero-Ferna'ndez L., A. Sa'enz-Herna'ez, J. Sanz-Asensio, P. Ferna'ndez-Zurbano, M. Sainz-Ram'rez, B. Pons-Jubera, M. Lo'pez-Alonso, S. Epifanio-Ferna'ndez and M. Mart'nez-Soria (2008) Determination of cyprodinil and fludioxonil in the fermentative process of must by high-performance liquid chromatography-diode array detection. J Sci. Food Agric. 88:1943-1948.   DOI   ScienceOn
14 農薬殘留分析研究班 (2006) 最新農薬 の殘留分析殘留分析 [改訂版], pp. 252-253, 中央法規出版
15 김강진, 김하석, 이대운, 이원 (1997) 분석화학 (제4판), pp. 698, 자유아카데미.
16 식품의약품안전청 (2010b) 식품공전
17 이영득 (2009) 식품공전 잔류농약분석법 실무 해설서 pp. 3, 78-79, 식품의약품안전청.
18 Cabras P., A. Angioni, V.L. Garau, M. Melis, F.M. Pirisi, E.V. Minelli, F. Cabitza and M. Cubeddu (1997) Fate of some new fungicides (cyprodinil, fludioxonil, pyrimethanil, and tebuconazole) from vine to wine. J. Agric. Food Chem. 45(7):2708-2710.   DOI   ScienceOn
19 Fong, W. G, H. A. Moye, J. N. Seiber and J. P. Toth (1999) Pesticide Residues in food: Methods, Technologies, and Regulations. Wiley Interscience. pp. 3-4, 40-44, Canada.
20 Dec J., K. Haider, A. Benesi, V. Rangaswamy, A. Schaffer, U. Plucken and J. M. Bollag (1997) Analysis of soil-bound residues of $^{13}C$-labeled fungicide cyprodinil by NMR spectroscopy. Environ. Sci. Technol. 31:1128-1135.   DOI   ScienceOn
21 Garau V. L., A. Angioni, A. Aguilera, A. A. Del Real, M. Russo and P. Cabras (2002) Disappearance of azoxystrobin, pyrimethanil, cyprodinil, and fludioxonil on tomatoes in a greenhouse. J Agric Food Chem 50:1929-1932.   DOI   ScienceOn
22 Heye, U.J., J. Speich, H. Siegle, R. Wohlhauser and A. Hubele (1994) CGA 219417-a novel broad-spectrum fungicide. Proc. Brighton Crop Prot. Conf. Pests Dis. 2:501-8.
23 Knight S. C., V. M. Anthony, A. M. Brady, A. J. Greenland, S. P. Heaney, D. C. Murray, K. A. Powell, M. A. Schulz, C. A. Spinks, P. A. Worthington and D. Youle (1997) Rationale and perspectives on the development of fungicides. Annu. Rev. Phytopathol. 35:349-72.   DOI   ScienceOn