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Effect of Inoculum Concentration of Rhizoctonia solani and Pythium ultimum Causing Damping-off of Pepper and Cucumber on the Efficacy of the Mixture of Etridiazole and Thiophanate-methyl  

Kim, Hyung-Jo (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
Jang, Ho-Sun (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
Lee, Soo-Min (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
Kim, Joo-Hyung (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
Shin, Jin-Ho (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
Kim, Heung-Tae (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
Publication Information
The Korean Journal of Pesticide Science / v.14, no.1, 2010 , pp. 65-71 More about this Journal
Abstract
This study was performed to investigate the inhibiting activity of etridiazole and thiophanate-mthyl on mycelial growth of Rhizoctonia solani and Pythium ultimum and the effect of inoculum density of each pathogen on the control efficacy of the mixture of etridiazole and thiophanate-methyl in a seedling assay test. In mycelial growth inhibition test, $EC_{50}$ values of etridiazole and thiophanate-methyl against R. solani were 15.87 and 9.34 ${\mu}g\;mL^{-1}$, while those were 0.2 and more than $500\;{\mu}g\;mL^{-1}$ against P. ultimum, respectively. Controlling activity of the mixture of etridiazole and thiophanate-methyl against damping-off of pepper and cucumber, caused by R. solani and P. ultimum was tested in a greenhouse. With the inoculation of R. solani, disease incidences of seedling in pepper were 73% and 95% at 0.5% and 1.0% of inoculum concentration, while in cucumber those were 55% and 62% at 1.0% and 2.0%. When P. ultimum was inoculated into soil by 2.0% of inoculum concentration, those in pepper and cucumber were 66.7% and 96.8%, respectively. The efficacy of the mixture was somehow affected by the concentration of R. solani. While each control value of the mixture was 94.4% and 90.7% in pepper and cucumber at low inoculum concentration (0.05%), the efficacy of the fungicide decreased in pepper and cucumber by 70.7% and 72.9% at high concentration of R. solani (0.1% in pepper and 0.5% in cucumber). However, the control value of the mixture was 100% in pepper and cucumber, irrespective of the inoculum concentration of P. ultimum, however, the increase of inoculum concentration in soil did not result in the decrease of the fungicide efficacy.
Keywords
the mixture of etridiazole and thiophanate-methyl; damping-off; inoculum concentration; red-pepper; cucumber;
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