Browse > Article

Enhanced Pathogenicity of Baculovirus Using Immunosuppressive Genes Derived From Cotesia plutellae Bracovirus  

Kim, Yong-Gyun (Department of Bioresource Sciences, College of Natural Sciences, Andong National University)
Kwon, Bo-Won (Central Research Institute, Kyung Nong Co.)
Bae, Sung-Woo (Department of Bioresource Sciences, College of Natural Sciences, Andong National University)
Choi, Jai-Young (Department of Entomology, College of Agriculture and Life Science, Seoul National University)
Je, Yeon-Ho (Department of Entomology, College of Agriculture and Life Science, Seoul National University)
Publication Information
The Korean Journal of Pesticide Science / v.12, no.3, 2008 , pp. 283-290 More about this Journal
Abstract
Baculoviruses have been used to control some serious lepidopteran pests. However, their narrow target insect spectrum and slow efficacy are main limitations to be used in various applications. This study introduces a technique to overcome these limitations by inhibiting insect immune defence to enhance the viral pathogenicity. Polydnaviruses are an insect DNA virus group and symbiotic to some ichneumonid and braconid endoparasitoids. Cotesia plutellae bracovirus (CpBV) is a braconid polydnavirus and encodes several immunosuppressive genes. We selected seven CpBV genes and recombined them to wild type Autographa California multiple nucleopolyhedrovirus (AcNPV). A bioassay of these seven recombinants indicated that most recombinants had similar or superior efficacy to wild type AcNPV against beet armyworm, Spodoptera exigua, and diamondback moth, Plutella xylostella. Recombinant AcNPV with CpBV-ELP was the most potent in terms of lethal time by shortening more than 2 days compared to wild type AcNPV. This recombinant was further proved in its dose-dependent pathogenicity and its efficacy by spray application on S. exigua infesting cabbage cultivated in pots. We discussed the efficacy of CpBV-ELP recombinant AcNPV in terms of suppressing antiviral activity of target insects.
Keywords
Baculovirus; Cotesia plutellae; Plutella xylostella; Polydnavirus; Recombinant; Spodoptera exigua;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Gho, H. K., S. G. Lee, B. P. Lee, K. M. Choi and J. H. Kim (1991) Simple mass-rearing of beet armyworm, Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae), on an artificial diet. Korean J. Appl. Entomol. 29:180-183
2 Ibrahim, A. M. A. and Y. Kim (2006) Parasitism by Cotesia plutellae alters the hemocyte population and immunological function of the diamondback moth, Plutella xylostella. J. Insect Physiol. 52:943-950   DOI   ScienceOn
3 Jung, S., M. Kwoen, J. Y. Choi, Y. H. Je and Y. Kim (2006) Parastism of Cotesia spp. enhances susceptibility of Plutella xylostella to other pathogens. J. Asia-Pac. Entomol. 9:255-263   과학기술학회마을   DOI   ScienceOn
4 Lavine, M. D. and M. R. Strand (2002) Insect hemocytes and their role in immunity. Insect Biochem. Mol. Biol. 32:1295-1309   DOI   ScienceOn
5 Lee, S., N. A. Basio, D. S. Kim and Y. Kim (2005) Proteomic analysis of parasitization by Cotesia plutellae against diamondback moth, Plutella xylostella. J. Asia-Pac. Entomol. 8:53-60   과학기술학회마을   DOI   ScienceOn
6 Mackauer, M. and P. Sequeira (1993) Patterns of development in insect parasites. pp. 1-23. In Parasites and Pathogens of Insects (Eds. N.E. Beckage, S.N. Thompson and G.A. Federici). Academic Press, New York
7 Meister, M., C. Hetru and J. A. Hoffmann (2000) The antimicrobial host defense of Drosophila. pp. 17-36. In Origin and Evolution of the Vertebrate Immune System. Current Topics in Microbiology (eds. L. Du Pasquier and G.W. Litman), Vol. 248, Springer-Verlag, Berlin, Germany
8 Quicke, D. L. J. (1997) Parasitic Wasps. Chapman & Hall, U.K
9 SAS Institute, Inc. (1989) SAS/STAT user's guide, Release 6.03, Ed. Cary, N.C
10 Washburn, J. O., B. A. Kirkpatrick and L. E. Volkman (1995) Comparative pathogenesis of Authographa californica M nuclear polyhedrosis virus in larvae of Trichoplusia ni and Heliothis virescens. Virology 209:561-568   DOI   ScienceOn
11 Krell, P. J., M. D. Summers and S. B. Vinson (1982) Virus with a multipartite superhelical DNA genome from the ichneumonid parasitoid Campoletis sonorensis. J. Virol. 43:859-870   PUBMED
12 Federici, B. A. and R. H. Rice (1997) Organization and molecular characterization of genes in the polyhedrin region of the Anagrapha falcifera multinucleocapsid NPV. Arch. Virol. 142:333-348   DOI   ScienceOn
13 Webb, B. A. 1998. Polydnavirus biology, genome structure, and evolution. pp. 105-139. In The Insect Virus (eds. L.K. Miller and L.A. Balls). Plenum, New York
14 김용균, N. Basio, A. M. A. Ibrahim, 배성우 (2006) 프루텔고치벌 브라코바이러스 (Cotesia plutellae bracovirus) 유래 IkB 유전자 구조와 피기생 배추좀나방 (Plutella xylostella) 체내 발현 패턴. 한응곤지 45:15-24   과학기술학회마을
15 김용균, 배상기, 이선영 (2004) 프루텔고치벌(Cotesia plutellae) 폴리드나바이러스 복제와 산란 습성. 한응곤지 43:225-231
16 김지민, M. Nalini, 김용균 (2008) 파밤나방(Spodoptera exigua)에 대한 곤충병원세균류 배양액의 곤충면역억제활성 및 비티 생물농약과 혼합효과. 농약과학회지 12:184-191   과학기술학회마을
17 Lowenberger, C. 2001. Innate immune response of Aedes aegypti. Insect Biochem. Mol. Biol. 31:219-229
18 Nalini, M., J. Y. Choi, Y. H. Je, I. Hwang and Y. Kim (2008) Immunoevasive property of a polydnaviral product, CpBV- lectin, protects the parasitoid egg from hemocytic encapsulation of Plutella xylostella (Lepidoptera: Yponomeutidae). J. Insect Physiol. 54:1125-1131   DOI   ScienceOn
19 Je, Y. H., J. H. Chang, J. Y. Rho and B. R. Jin (2001) Generation of baculovirus expression vector using defective Autographa californica nuclear polyhedrosis virus genome maintained in Escherichia coli for $Occ^{+}$ virus production. Int. J. Indust. Entomol. 2:155-160
20 Kirkpatrick, B. A., J. O. Washburn and L. E. Volkman (1998) AcMNPV pathogenesis and developmental resistance in fifth instar Heliothis virescens, J, Invertebr. Pathol. 72:63-72   DOI   ScienceOn
21 Kroemer, J. A. and B. A. Webb (2004) Polydnavirus genes and genomes: emerging gene families and new insights into polydnavirus replication. Annu. Rev. Entomol. 49:431-456   DOI   ScienceOn
22 Ibrahim, A. M. A. and Y. Kim (2008) Transient expression of protein tyrosine phosphatases encoded in Cotesia plutellae bracovirus inhibits insect cellular immune responses. Naturwissenschaften 95:25-32   DOI
23 Lee, S., M. Nalini and Y. Kim (2008) A viral lectin encoded in Cotesia plutellae bracovirus and its immunosuppressive effect on host hemocytes. Comp. Biochem. Physiol. 149A: 351-361
24 Webb, B. A. and M. R. Strand (2005) The biology and genomes of polydnavirus. pp. 323-360. In Comprehensive Molecular Insect Science (eds. L.I. Gilbert, K. Iatrou and S.S. Gill), Elsevier, New York
25 Basio, N. A. M and Y. Kim (2006) Additive effect of teratocyte and calyx fluid from Cotesia plutellae on immunosuppression of Plutella xylostella. Physiol. Entomol. 31:341-347   DOI   ScienceOn
26 Lee, S. and Y. Kim (2008) Two homologous parasitism-specific proteins encoded in Cotesia plutellae bracovirus and their expression profiles in parasitized Plutella xylostella. Arch. Insect Biochem. Physiol. 67:157-171   DOI   ScienceOn
27 Raymond, M. (1985) Presentation d'un programme d'analyse log-probit pour micro-ordinateur. Cah. ORS-TOM. Ser. Ent. Med. et Parasitol. 22:117-121
28 Kim, Y. and S. Ryu (2007) Ultrastructure of Cotesia plutellae bracovirus in its replication at wasp ovarian calyx. J. Asia-Pac. Entomol. 10:357-361   과학기술학회마을   DOI   ScienceOn
29 Espagne, E., C. Dupuy, E. Huguet, L. Cattolico, B. Provost, N. Martins, M. Poirie, G. Periquet and J. M. Drezen (2004) Genome sequence of a polydnavirus: insights into symbiotic virus evolution. Science 306:286-289   DOI   ScienceOn
30 Washburn, J. O., J. F. Wong and L. E. Volkman (2001) Comparative pathogenesis of Helicoverpa zea S nucleopolyhedrovirus in noctuid larvae. J. Gen. Virol. 82:1777-1784   DOI   PUBMED
31 Clem, R. J. (2001) Baculoviruses and apoptosis: the good, the bad, and the ugly. Cell Death Differ. 8:137-143   DOI
32 Kim, Y., J. Y. Choi and Y. H. Je (2007) Cotesia plutellae bracovirus genome and its function in altering insect physiology. J. Asia-Pac. Entomol. 10:181-191   과학기술학회마을   DOI   ScienceOn
33 Nalini, M. and Y. Kim (2007) A putative protein translation inhibitory factor encoded by Cotesia plutellae bracovirus suppresses host hemocyte-spreading behavior. J. Insect Physiol. 53:1283-1292   DOI   ScienceOn
34 O'Reilly, D. R., L. K. Miller and V.A. Luckow (1992) Baculovirus expression vectors: a laboratory manual. Oxford University, New York
35 Gad, W. and Y. Kim (2008) A viral histone H4 encoded by Cotesia plutellae bracovirus inhibits haemocyte-spreading behaviour of the diamondback moth, Plutella xylostella. J. Gen. Virol. 89:931-938   DOI   ScienceOn
36 Gillespie, J. P., M. R. Kanost and T. Trenczek (1997) Biological mediators of insect immunity. Annu. Rev. Entomol. 42:611-643   DOI   ScienceOn
37 Kwon, B. and Y. Kim (2008) Transient expression of an EP1-like gene encoded in Cotesia plutellae bracovirus suppresses the hemocyte population in the diamondback moth, Plutella xylostella. Dev. Comp. Immunol. 32:932-942   DOI   ScienceOn