Inheritance and Cross Resistance of Acequinocyl Resistance in Twospotted Spider Mite, Tetranychus urticae

The development of resistance to acequinocyl was found in the population of the twospotted spider mite, Tetranychus urticae, collected from rose greenhouses in Gimhae, Gyeongnam province in January 2001. This pest is reared on 5 years treated with acequinocyl (over 200 times), and increased 87.8 folds in resistance as compared to susceptible strain (S). Inheritance of acequinocyl resistant strain (R) and cross resistance of this strain to 8 acaricides against T. urticae adults and eggs was investigated. There were differences of susceptibility in the acequinocyl concentration-mortality relationships in $F_1$ progenies obtained from reciprocal cross with the S and R strain ($S(female){\times}R(male)$, $R(female){\times}S(male)$). Degrees of dominance were -0.75, -0.57 in $F_1$ progenies of adult and egg of $S(female){\times}R(male)$. Inheritance in $F_1$ progenies of $S(female){\times}R(male)$ was incomplete recessive. Degree of dominance were 0.81, 0.45 in $F_1$ progenies of adult and egg of $R(female){\times}S(male)$, respectively. These results suggest that inheritance of acequinocyl resistance is controlled by a complete dominance. The R strain exhibited cross resistance of 1.1 and 0.9 fold to amitraz, bifenazate, and negatively correlated cross resistance of 0.08 fold to emamectin benzoate in adult females. The R strain showed cross resistance of 37.7, 14.0, and 26.2 fold to amitraz, milbemectin and spriodiclofen in eggs, respectively. Particularly it showed high levels of cross-resistance to pyridaben with 6538.3 fold. These chemicals showed negatively correlated cross-resistance exhibited 0.4, 0.3, and 0.2 fold to ahamectin, bifenazate, and emamectin benzoate in eggs.

Acequinocyl 저항성 점박이응애의 유전과 교차저항성

경남 김해의 장미재배지에서 채집한 점박이응애를 실험실에서 5년 동안 acequinocyl로 200회 이상 도태하여 성충에 대해 감수성계통과 비교하여 87.8배의 저항성계통을 얻었다. 점박이응애의 성충과 알에 대해 aceqyinocyl 저항성 계통의 유전과 8종 살비제에 대한 교차저항성 유무를 조사하였다. 감수성계통 수컷과 저항성계통 암컷을 상호교배($S(female){\times}R(male)$)하여 얻은 $F_1$ 알과 성충의 우성도는 각각 -0.75 -0.57로 불완전열성이었고, $R(female){\times}S(male)$의 교배에서 얻어진 $F_1$ 알과 성충의 우성도는 각각 0.81, 0.45로 불완전우성이었다. 이 저항성 점박이응애의 성충은 amitraz, bifenazate에 대해 각각 1.1, 0.9배로 비교차저항성을 나타내었으며, emamectin benzoate에 대해서는 0.08배로 역상관 교차저항성을 나타내었다. 알은 amitraz, milbemectin, spirodiclofen에 각각 37.7, 14.0, 26.2배로 교차저항성을 나타내었고, 특히 pyridaben은 6538.3배로 아주 높은 교차저항성을 나타내었다. 역상관 교차저항성을 나타내는 약제들은 abamectin, bifenazate, emamectin benzoate로서 알에 대해 각각 0.4, 0.3, 0.2배를 나타내었다.