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Determination of paraquat-resistant biotype on Conyza canadensis and the resistant mechanism  

Kim, Sung-Eun (Faculty of Biotechnology and Research Center of Bioactive Materials, Chonbuk National University)
Kim, Seung-Yong (Faculty of Biotechnology and Research Center of Bioactive Materials, Chonbuk National University)
Ahn, Sul-Hwa (Faculty of Biotechnology and Research Center of Bioactive Materials, Chonbuk National University)
Chun, Jae-Chul (Faculty of Biotechnology and Research Center of Bioactive Materials, Chonbuk National University)
Publication Information
The Korean Journal of Pesticide Science / v.9, no.1, 2005 , pp. 88-96 More about this Journal
Abstract
Paraquat-resistant biotype of Conyza canadensis (L.) Cronq. was determined by chlorophyll loss and random amplified polymorphic DNA (RAPD) analysis and the resistant mechanism was investigated with respect to absorption, translocation, and binding constant. RAPD analysis for paraquat resistant (R) and susceptible (S) biotypes found in a pear orchard revealed that the biotypes possessed remote genetic relationship. Chlorophyll loss, as an indication of paraquat toxicity, of S biotype was 7.8-fold greater than that of R biotype. There were no differences in contents of epicuticular wax and cuticle and amounts of [14C]paraquat penetrating the cuticle between the two biotypes. Little translocation of the herbicide out of the treated leaf was observed in either biotype. Binding constants of paraquat to the cell wall and thylakoid membrane were 7.4-fold and 16.9-fold, respectively, higher in R biotype than in S biotype. The results suggest that the resistance mechanism of C. canadensis biotype is due partly to high binding affinity of paraquat to the cell wall and thylakoid membrane.
Keywords
Paraquat-resistant biotype; Conyza canadensis; Resistant mechanism; paraquat; thylakoid;
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1 Chun, J. C., H. J. Lee, S. J. Lim, S. E. Kim, and J. O. Guh (2001) Comparative absorption, translocation, and metabolism of foliar applied oxyfluorfen in wheat and barley. Pestic. Biochem. Physiol. 70:118-125   DOI   ScienceOn
2 Chun, J. C., S. Y. Ma, S. E. Kim, and H. J. Lee (1997) Physiological responses of Rehmannia glutinosa to paraquat and its tolerance mechanisms. Pestic. Biochem. Physiol. 59:51-63   DOI   ScienceOn
3 Fuerst, E. P. and K. C. Vaughn (1990) Mechanisms of paraquat resistance. Weed Technol. 4, 150-156
4 Norman, M. A., R. J. Smeda, and K. C. Vaughn (1993) Evaluation of paraquat resistance mechanisms in Conyza. Pestic. Biochem. Physiol. 46:236-249   DOI   ScienceOn
5 김희주, 황을철 (2000) Paraquat 저항성 망초의 protective 효소. 한국농화학회지 43(1):46-51
6 Preston, C. (1994) Resistance to photosystem disrupting herbicide. pp.61-82. In S. B. Powles and A. M. Holtum, eds. Herbicide Resistance in Plants: Biology and Biochemistry. Lewis Publishers, Boca Raton, FL
7 Maxwell, B. D. and A. M. Mortimer (1994) Selection for herbicide resistance. pp.1-25. In S. B. Powles and A. M. Holtum, eds. Herbicide Resistance in Plants: Biology and Biochemistry. Lewis Publishers, Boca Raton, FL
8 Youngman, R. J. and A. D. Dodge (1981) On the mechanism of paraquat resistance in Conyza sp. pp.537-544. In G. Akoyunoglou, ed. Photosynthesis. IV. Photosynthesis and Productivity, Photosynthesis and Environment. Balaban International Sceince Service, Philadelphia, PA
9 Hiscox, J. D. and G. F. Israelstam (1979) A method for the extraction of chlorophyll from leaf tissues without marceration. Can. J. Bot. 57:1332-1335   DOI
10 Kim, S. and K. K. Hatzios (1993) Competitive uptake, translocation, and metabolism of paraquat in tolerant Kwangkyo and susceptible Hood soybean. Pestic. Biochem. Physiol. 47:149-158   DOI   ScienceOn
11 변종영, 박인철, 노석원 (2001) 한국에서 paraquat 저항성 망초의 출현 및 분포. 한잡초지 21(1):27-32
12 Ricotta, J. A. and J. B. Masiunas (1992) Relationship of leaf surface characteristics to acifluorfen tolerance in tomato (Lycopersicon esculentum) and related species. Weed Sci. 40:402-407
13 Suntres, Z. E. (2002) Role of antioxidants in paraquat toxicity. Toxicology 180:65-77   DOI   PUBMED   ScienceOn