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http://dx.doi.org/10.5307/JBE.2005.30.4.249

Development of an Enzyme Electrode Biosensor for Lactic Acid Bacteria  

Park T. S. (School of Biosystems Engineering, Seoul National University)
Cho S. I. (School of Biosystems Engineering, Seoul National University)
Publication Information
Journal of Biosystems Engineering / v.30, no.4, 2005 , pp. 249-253 More about this Journal
Abstract
This study was carried out to develop enzyme biosensor for lactic acid bacteria. Lactic acids produced by lactic acid bacteria (LAB) was measured and good correlation $R^2=0.98$ between LAB count and lactic acids concentration was found. Hydrogen ion produced by L-lactate dehydrogenase (L-LDH) was measured by a potentiometer. Glutamic-pyruvic transminase (GPT) was used for eliminating inhibitor in the reaction. Polyacrylamide gel was used for immobilizing matrix of the sensor. The biosensor was tested and showed good feasibility with $R^2=0.99$ on validation.
Keywords
Biosensor; Enzyme; Lactic acid bacteria; Lactate; Clutamic-pyruvic transminase;
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