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http://dx.doi.org/10.5307/JBE.2003.28.6.505

Enzyme Immunoassay for On-line Sensing of the Insecticide Imidaclopird Residues  

송석진 (Department of Agricultural Machinery Engineering, Chungbuk National University)
조한근 (Department of Agricultural Machinery Engineering, Chungbuk National University)
Publication Information
Journal of Biosystems Engineering / v.28, no.6, 2003 , pp. 505-510 More about this Journal
Abstract
In Korea, due to its broad efficacy as a systemic insecticide, imidacloprid has been widely used in rice paddies to control sucking insects, soil insects, and some chewing insects and in apple orchards to control various insects pests. To quantify the imidacloprid residue concentrations, samples are assayed in vitro using enzyme-linked immunosorbent assays(ELISA). These assays generally require several hours to perform. As a biosensor, a competitive imidacloprid ELISA was modified to measure insecticide concentrations. It was found that a total assay time of 15 min(10-min antibody-antigen binding, and 5-min substrate development) is sufficient for monitoring imidacloprid concentrations. Further work is needed to improve the sensitivity of the measurement protocol.
Keywords
Biosensor; ELISA; Antibody; Antigen; Imidacloprid; Insecticide;
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