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http://dx.doi.org/10.48022/mbl.2202.02009

Comparative Transcriptome Analysis of Zerumbone-Treated Helicobacter pylori  

Woo, Hyun Jun (Department of Clinical Laboratory Science, Semyung University)
Yang, Ji Yeong (Division of Crop Foundation, National Institute of Crop Science (NICS), Rural Development Administration (RDA))
Kim, Sa-Hyun (Department of Clinical Laboratory Science, Semyung University)
Publication Information
Microbiology and Biotechnology Letters / v.50, no.2, 2022 , pp. 301-309 More about this Journal
Abstract
Helicobacter pylori (H. pylori) establishes infection in the human gastric mucosa for a long time and causes severe gastric diseases such as peptic ulcer and gastric cancer. When H. pylori is exposed to the antibacterial agents or inhibitors, the expression of pathogenic associated genes could be altered. In this study, we analyzed the transcriptional changes of H. pylori genes induced by zerumbone treatment. RNA expression changes were analyzed using next-generation sequencing (NGS), and then reverse transcription-polymerase chain reaction (RT-PCR) was performed to verify the results. As a result of NGS analysis, a total of 23 out of 1,632 genes were differentially expressed by zerumbone treatment. RT-PCR confirmed that zerumbone treatment regulated the expression level of 14 genes. Among the genes associated with DNA replication, transcription, virulence factors and T4SS components, 10 genes (dnaE, dnaQ, rpoA, rpoD, secA, flgE, flhA, virB5, virB8 and virB9) were significantly down-regulated and 4 genes (flaA, flaB, virB4 and virD4) were up-regulated. The results of our current study imply that zerumbone might be a potential therapeutic agent for H. pylori infection by regulating factors related to various H. pylori pathogenicity.
Keywords
Helicobacter pylori; zerumbone; next-generation sequencing; RNA-sequencing;
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