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http://dx.doi.org/10.48022/mbl.2102.02005

Ambient Air Waste Sorting Facilities Could Be a Source of Antibiotic Resistant Bacteria  

Calheiros, Ana (Escola Superior de Tecnologia e Gestao, Instituto Politecnico de Viana do Castelo, Rua Escola Industrial e Comercial de Nun'Alvares)
Santos, Joana (Escola Superior de Tecnologia e Gestao, Instituto Politecnico de Viana do Castelo, Rua Escola Industrial e Comercial de Nun'Alvares)
Ramos, Carla (Escola Superior de Tecnologia e Gestao, Instituto Politecnico de Viana do Castelo, Rua Escola Industrial e Comercial de Nun'Alvares)
Vasconcelos, Marta (CISAS, Escola Superior de Tecnologia e Gestao, Instituto Politecnico de Viana do Castelo, Rua Escola Industrial e Comercial de Nun'Alvares)
Fernandes, Paulo (Escola Superior de Tecnologia e Gestao, Instituto Politecnico de Viana do Castelo, Rua Escola Industrial e Comercial de Nun'Alvares)
Publication Information
Microbiology and Biotechnology Letters / v.49, no.3, 2021 , pp. 367-373 More about this Journal
Abstract
The antimicrobial resistance of Staphylococcus spp. and Gram negative strains present in air samples from waste sorting facilities was assessed. Phenotypic studies have revealed a high percentage of strains of Staphylococcus spp. resistant to methicillin. Genotypically and by RT-PCR, it was found that the mecA gene usually associated with methicillin resistance was present in 8% of the Staphylococcus strains isolated. About 30% of the Gram negative strains from the same samples also displayed resistance to meropenem and 79% of these were resistant to multiple antibiotics from different classes, namely cephalosporins and β-lactams. The results suggest that in professional activities with high levels of exposure to biological agents, the quantification and identification of the microbial flora in the work environment, with the determination of the presence of potential agents displaying multi-resistances is of relevance to the risk assessment. The personal protection of workers is particularly important relevance in these cases, since many of the strains that exhibit multi-resistance are potential opportunistic agents.
Keywords
Staphylococcus; Enterobacteriaceae; biological risk; antimicrobial resistance;
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1 Nascimento TC, Januzzi WD, Leonel M, da Silva VL, Diniz CG. 2009. Occurrence of clinically relevant bacteria in health service waste in a Brazilian sanitary landfill and antimicrobial susceptibility profile. Rev. Soc. Bras. Med. Trop. 42: 415-419.   DOI
2 Laurent F, Chardon H, Haenni M, Bes M, Reverdy M-E, Madec J-Y, et al. 2012. MRSA harboring mecA variant gene mecC, France. Emerg. Infect. Dis. 18: 1465-1467.   DOI
3 Perazzi B, Fermepin MR, Malimovka A, Garcia SD, Orgambide M, Vay CA, et al. 2006. Accuracy of cefoxitin disk testing for characterization of oxacillin resistance mediated by penicillin-binding protein 2a in coagulase-negative Staphylococci. J. Clin. Microbiol. 44: 3634-3639.   DOI
4 Ghoshal U, Prasad KN, Singh M, Tiwari DP, Ayyagari A. 2004. A comparative evaluation of phenotypic and molecular methods for the detection of oxacillin resistance in coagulase-negative staphylococci. J. Infect. Chemother. 10: 86-89.   DOI
5 Elhassan MM, Ozbak HA, Hemeg HA, Elmekki MA, Ahmed LM. 2015. Absence of the mecA Gene in methicillin resistant Staphylococcus aureus isolated from different clinical specimens in Shendi City, Sudan. BioMed. Res. Int. 2015: 895860-895860.
6 Brooks JP, Maxwell SL, Rensing C, Gerba CP, Pepper IL. 2007. Occurrence of antibiotic-resistant bacteria and endotoxin associated with the land application of biosolids. Can. J. Microbiol. 53: 616-622.   DOI
7 Pitout JD, Laupland KB. 2008. Extended-spectrum beta-lactamaseproducing Enterobacteriaceae: an emerging public-health concern. Lancet Infect. Dis. 8: 159-166.   DOI
8 Paterson DL. 2006. Resistance in gram-negative bacteria: enterobacteriaceae. Am. J. Med. 119: S20-S28.   DOI
9 Douwes J, Thorne P, Pearce N, Heederik D. 2003. Bioaerosol health effects and exposure assessment: progress and prospects. Ann. Occup. Hyg. 47: 187-200.   DOI
10 Brun E. 2007. Expert forecast on emerging biological risks related to occupational safety and health. Luxembourg: EUR-OP.
11 DTU Food NFI, European Union Reference Laboratory-Antibiotic Resistance. 2012. Protocol for PCR Amplification of mecA, mecC (mecALGA251), spa and pvl. https://www.eurl-ar.eu/CustomerData/Files/Folders/21-protocols/279_pcr-spa-pvl-meca-meccsept12. pdf. Accessed Nov. 16, 2017.
12 Cohen Stuart J, Leverstein-Van Hall MA. 2010. Guideline for phenotypic screening and confirmation of carbapenemases in Enterobacteriaceae. Int. J. Antimicrob. Agents 36: 205-210.   DOI
13 Fontes CO, Silva VL, de Paiva MRB, de Paiva B, Garcia RA, Resende JA, et al. 2013. Prevalence, antimicrobial resistance, and virulence characteristics of mecA-encoding coagulase-negative Staphylococci isolated from soft cheese in Brazil. J. Food Sci. 78: M594-M599.
14 Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, et al. 2012. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin. Microbiol. Infect. 18: 268-281.   DOI
15 Leclercq R, Canton R, Brown DFJ, Giske CG, Heisig P, MacGowan AP, et al. 2013. EUCAST expert rules in antimicrobial susceptibility testing. Clin. Microbiol. Infect. 19: 141-160.   DOI
16 CLSI. 2012. Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard-11th, Ed. CLSI document M02-A11.
17 The European Comittee on Antimicrobial Susceptibility Testing. 2014. Breakpoint tables for interpretation of MICs and zone diameters. Version 4.0, 2014. http://www.eucast.org.
18 CLSI. 2012. Methods for Dilution Antimicrobial Susceptibility Tests f or Bacteria That Grow Aerobically; Approved Standard-9th, Ed. CLSI document M07-A9.
19 Kalia A, Rattan A, Chopra P, Kalia A, Rattan A, Chopra P. 1999. A method for extraction of high-quality and high-quantity genomic DNA generally applicable to pathogenic bacteria. Anal. Biochem. 275: 1-5.   DOI
20 Murakami K, Minamide W, Wada K, Nakamura E, Teraoka H, Watanabe S. 1991. Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction. J. Clin. Microbiol. 29: 2240-2244.   DOI
21 Muyzer G, Dewaal EC, Uitterlinden AG. 1993. Profiling of complex microbial-populations by denaturing gradient gel-electrophoresis analysis of polymerase chain reaction-amplified genes-coding for 16s ribosomal-RNA. Appl. Environ. Microbiol. 59: 695-700.   DOI
22 Bhargava K, Zhang Y. 2012. Multidrug-resistant coagulase-negative Staphylococci in food animals. J. Appl. Microbiol. 113: 1027-1036.   DOI
23 Pinto MJdV, Veiga JM, Fernandes P, Ramos C, Goncalves S, Velho MMLV, et al. 2015. Airborne microorganisms associated with packaging glass sorting facilities. J. Toxicol. Environ. Health Part A. 78: 685-696.   DOI
24 Anderson M, Clift C, Schulze K, Sagan A, Nahrgang S, Alt Ouakrim D, et al. 2019. Averting the AMR Crisis: What are the Avenues for Policy Action for Countries in Europe. In POLICY BRIEF 32, European Observatory on Health Systems and Policies, WHO.
25 World Health Organization. 2020. Antibiotic Resistance. Available from https://www.who.int/news-room/fact-sheets/detail/antibiotic-resistance. Accessed Jan. 30, 2020.
26 Berendonk TU, Manaia CM, Merlin C, Fatta-Kassinos D, Cytryn E, Walsh F, et al. 2015. Tackling antibiotic resistance: the environmental framework. Nat. Rev. Microbiol. 13: 310-317.   DOI
27 Matuschek E, Brown DF, Kahlmeter G. 2014. Development of the EUCAST disk diffusion antimicrobial susceptibility testing method and its implementation in routine microbiology laboratories. Clin. Microbiol. Infect. 20: O255-266.   DOI