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http://dx.doi.org/10.48022/mbl.2002.02019

Characterization of the Recombinant Glutamate Decarboxylase of Lactobacillus brevis G144 Isolated from Galchi Jeotgal, a Korean Salted and Fermented Seafood  

Kim, Jeong A (Institute of Agriculture and Life Science, Graduate School, Gyeongsang National University)
Park, Ji Yeong (Division of Applied Life Science BK21 4, Graduate School, Gyeongsang National University)
Kim, Jeong Hwan (Institute of Agriculture and Life Science, Graduate School, Gyeongsang National University)
Publication Information
Microbiology and Biotechnology Letters / v.49, no.1, 2021 , pp. 9-17 More about this Journal
Abstract
A γ-aminobutyric acid (GABA)-producing microorganism was isolated from galchi (hairtail fish, Trichiurus lepturus) jeotgal, a Korean salted and fermented seafood. The G144 isolate produced GABA excessively when incubated in MRS broth containing monosodium glutamate (MSG, 3%, w/v). G144 was identified as Lactobacillus brevis through 16S rRNA and recA gene sequencing. gadB and gadC encoding glutamate decarboxylase (GAD) and glutamate/GABA antiporter, respectively, were cloned and gadB was located downstream of gadC. The operon structure of gadCB was confirmed by reverse transcription (RT)-polymerase chain reaction. gadB was overexpressed in Escherichia coli and recombinant GAD was purified and its size was 54.4 kDa as evidenced by SDS-PAGE results. Maximum GAD activity was observed at pH 5.0 and 40℃ and the activity was dependent on pyridoxal 5'-phophate. The Km and Vmax of GAD were 8.6 mM and 0.01 mM/min, respectively.
Keywords
GABA; Lactobacillus brevis; glutamate decarboxylase; galchi jeotgal;
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