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http://dx.doi.org/10.48022/mbl.2009.09005

Bioethanol Production from Gracilaria verrucosa Using Saccharomyces cerevisiae with Adaptive Evolution  

Yang, Ji Won (Department of Biotechnology, Pukyong National University)
Park, Yu Rim (Department of Biotechnology, Pukyong National University)
Jeong, Gwi-Taek (Department of Biotechnology, Pukyong National University)
Kim, Sung-Koo (Department of Biotechnology, Pukyong National University)
Publication Information
Microbiology and Biotechnology Letters / v.49, no.1, 2021 , pp. 88-94 More about this Journal
Abstract
The seaweed, Gracilaria verrucosa (red seaweed) was fermented to produce bioethanol. Optimal thermal acid hydrolysis conditions were determined as 200 mM H2SO4 and 10% (w/v) seaweed slurry at 130℃ for 60 min yielding 47.5% of pretreatment efficiency (Ep). After the thermal acid hydrolysis, enzymatic saccharification was carried out with 16 U/ml Viscozyme L, Cellic CTec2 or mixture of Viscozyme L and Cellic CTec2 to G. verrucosa hydrolysates. Enzymatic saccharifications with Viscozyme, Cellic CTec2 or mixture of those yielded 7.3 g/l glucose with efficiency of saccharification, Es = 34.9%, 11.6 g/l glucose with Es = 64.4% and the mixture of those 9.6 g/l glucose with Es = 56.6%, respectively. Therefore, based on the Es value, Cellic CTec2 was selected for the optimal enzyme for enzymatic saccharification of G. verrucosa hydrolysate. The ethanol productions with non-adapted S. cerevisiae CEN-PK2 (wild type) and S. cerevisiae CEN-PK2 with adaptive evolution to galactose produced 8.5 g/l ethanol with YEtOH = 0.19 and 21.5 g/l ethanol with YEtOH = 0.50 at 144 h, respectively. From these results, the ethanol production by S. cerevisiae with adaptive evolution showed high concentration of ethanol production using G. verrucosa as a substrate.
Keywords
Bioethanol; red seaweed; Gracilaria verrucosa; ethanol fermentation;
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1 Guo Z, Olsson L. 2014. Physiological response of Saccharomyces cerevisiae to weak acids present in lignocellulosic hydrolysate. FEMS Yeast. Res. 14: 1234-1248.   DOI
2 Linihan P, Orozco A, O'Neill E, Ahmad MNM, Rooney DW, Walker GM. 2010. Dilute acid hydrolysis of lignocellulosic biomass. Chem. Eng. J. 156: 395-403.   DOI
3 Cazetta ML, Celligoi MAPC, Buzato JB, Scarmino IS. 2007. Fermentation of molasses by Zymomonas mobilis: effects of temperature and sugar concentration on ethanol production. Bioresour. Technol. 98: 2824-2828.   DOI
4 Dias MOS, Esinas AV, Nebra SA, Filho RM, Rossel CEV, Maciel MRW. 2009. Production of bioethanol and other bio-based materials from sugarcane bagasse. Chem. Eng. Res. Des. 87: 1206-1216.   DOI
5 Adamas JM, Gallagher JA, Donnison LS. 2009. Fermentation study on Saccharina latissimi for bioethanol production considering variable pre-treatments. J. Appl. Phycol. 21: 569.   DOI
6 Sanchez-Machado DI, Lopez-Cervantes J, Paseiro-Losada P, Lopez-Hernandez J. 2004. Fatty acids, total lipid, protein and ash contents of processed edible seaweeds. Food. Chem. 85: 439-444.   DOI
7 Qi L, Mui YF, Lo SW, Lui MY, Akien GR, Horvath IT. 2014. Catalytic conversion of fructose, glucose and sucrose to 5-(hydroxymethyl) furfural and levulinic and formic acids in γ-valerolactone as a green solvent. ACS. Catal. 4: 1470-1477.   DOI
8 Ra CH, Choi JG, Kang CH, Sunwoo IY, Jeong GT, Kim SK. 2015. Thermal acid hydrolysis pretreatment, enzymatic saccharification and ethanol fermentation from red seaweed, Gracilaria verrucosa. Microbiol. Biotechnol. Lett. 41: 9-15.
9 Agbor VB, Cicek N, Sparling R, Berlin A, Levin DB. 2011. Biomass pretreatment: fundamentals toward application. Biotechnol. Adv. 29: 675-685.   DOI
10 Daroch M, Geng S, Wang G. 2013. Recent advances in liquid biofuel production from algal feedstocks. Appl. Energy 102: 1371-1381.   DOI
11 Chiaramonti D, Prissi M, Ferrero S, Oriani L, Ottonello P, Torre P, et al. 2012. Review of pretreatment processes for lignocellulosic ethanol production. Biomass Bioenergy 46: 25-35.   DOI
12 Kim MJ, Kim SK. 2012. Ethanol production by separate hydrolysis and fermentation and simultaneous saccharification and fermentation using Saccharina japonica. KSBB J. 27: 86-90.   DOI
13 Morimoto S, Murakami M. 1993. Biotransformation of furfural and 5-hydroxymethyl furfural by enteric bacteria. J. Ind. Microbiol. 11: 147-150.   DOI
14 Cho Y, Ra CH, Kim SK. 2014. Ethanol production from the seaweed Gelidium amansii, using specific sugar acclimated yeasts. J. Microbiol. Biotechnol. 24: 264-269.   DOI
15 Cho Y, Kim H, Kim SK. 2013. Bioethanol production from brown seaweed, Undaria pinnatifida, using NaCl acclimated yeast. Bioprocess Biosyst. Eng. 36: 713-719.   DOI
16 Sabourin-Provost G, Hallenbeck PC. 2009. High yield conversion of a crude glycerol fraction from biodiesel production to hydrogen by photofermentation. Bioresour. Technol. 100: 3513-3517.   DOI
17 Yazdani SS, Gonzalez R. 2007. Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry. Curr. Opin. Biotechnol. 18: 213-219.   DOI
18 Bothast RJ, Schlicher MA. 2005. Biotechnological processes for conversion of corn into ethanol. Appl. Microbiol. Biotechnol. 67: 19-25.   DOI