Browse > Article
http://dx.doi.org/10.48022/mbl.2010.10001

Screening of Myxobacteria Carrying Tubulysin Biosynthetic Genes  

Hyun, Hyesook (Department of Biotechnology, Hoseo University)
Choi, Juo (Department of Biotechnology, Hoseo University)
Kang, Daun (MECOX CureMed Co.)
Kim, Yungpil (MECOX CureMed Co.)
Lee, Pilgoo (MECOX CureMed Co.)
Chung, Gregory J.Y. (MECOX CureMed Co.)
Cho, Kyungyun (Department of Biotechnology, Hoseo University)
Publication Information
Microbiology and Biotechnology Letters / v.49, no.1, 2021 , pp. 32-38 More about this Journal
Abstract
Tubulysins are a group of secondary metabolites produced by myxobacteria that inhibit the function of the eukayotic cytoskeleton. We developed a pair of PCR primers that specifically amplified tubulysin biosynthetic genes. Using these primers, eight out of the eighty-one strains of myxobacteria belonging to the Cystobacteraceae family that harbored putative tubulysin biosynthetic genes were screened through PCR analysis. The selected strains included two Archangium gephyra, two Stigmatella sp., two Vitiosangium cumulatum, and two unidentified myxobacteria. LC-MS analysis of the culture extracts from the selected strains revealed that A. gephyra KYC4066 produced putative tubulysin A and B.
Keywords
Archangium gephyra; myxobacteria; secondary metabolite; tubulysin;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Szigetvari NM, Dhawan D, Ramos-Vara JA, Leamon CP, Klein PJ, Ruple AA, et al. 2018. Phase I/II clinical trial of the targeted chemotherapeutic drug, folate-tubulysin, in dogs with naturallyoccurring invasive urothelial carcinoma. Oncotarget 9: 37042-37053.   DOI
2 Hyun H, Cho K. 2018. Secondary metabolites of myxobacteria. Korean J. Microbiol. 54: 175-187.   DOI
3 Sasse F, Steinmetz H, Heil J, Hofle G, Reichenbach H. 2000. Tubulysins, new cytostatic peptides from myxobacteria acting on microtubuli. Production, isolation, physico-chemical and biological properties. J. Antibiot. 53: 879-885.   DOI
4 Reddy JA, Dorton R, Bloomfield A, Nelson M, Dircksen C, et al. 2018. Pre-clinical evaluation of EC1456, a folate-tubulysin anticancer therapeutic. Sci. Rep. 8: 8943.   DOI
5 Murray BC, Peterson MT, Fecik RA. 2015. Chemistry and biology of tubulysins: antimitotic tetrapeptides with activity against drug resistant cancers. Nat. Prod. Rep. 32: 654-662.   DOI
6 Sandmann A, Sasse F, Muller R. 2004. Identification and analysis of the core biosynthetic machinery of tubulysin, a potent cytotoxin with potential anticancer activity. Chem. Biol. 11: 1071-1079.   DOI
7 Gerth K, Pradella S, Perlova O, Beyer S, Muller R. 2003. Myxobacteria: proficient producers of novel natural products with various biological activities-past and future biotechnological aspects with the focus on the genus Sorangium. J. Biotechol. 106: 233-253.   DOI
8 Schaberle TF, Lohr F, Schmitz A, Konig GM. 2014. Antibiotics from myxobacteria. Nat. Prod. Rep. 31: 953-972.   DOI
9 Gerth K, Bedorf N, Hofle G, Irschik H, Reichenbach H. 1996. Epothilons A and B: antifungal and cytotoxic compounds from Sorangium cellulosum (Myxobacteria). Production, physicochemical and biological properties. J. Antibiot. 49: 560-563.   DOI
10 Stein A. 2010. Ixabepilone. Clin. J. Oncol. Nurs. 14: 65-71.   DOI
11 Chai Y, Pistorius D, Ullrich A, Weissman KJ, Kazmaier U, Muller R. 2010. Discovery of 23 natural tubulysins from Angiococcus disciformis An d48 and Cystobacter SBCb004. Chem. Biol. 17: 296-309.   DOI
12 Reichenbach H. 2005. Myxococcales, pp. 1059-1144. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (eds.), Bergey's manual of systematic bacteriology, 2nd Ed. Bergey's Manual Trust, East Lansing, MI, USA.
13 Shin H, Youn J, An D, Cho K. 2013. Production of antimicrobial substances by strains of myxobacteria Corallococcus and Myxococcus. Korean J. Microbiol. Biotechnol. 41: 44-51.   DOI
14 Rainey FA, Ward-Rainey N, Kroppenstedt RM, Stackebrandt E. 1996. The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int. J. Syst. Bacteriol. 46: 1088-1092.   DOI
15 Han K, Li ZF, Peng R, Zhu LP, Zhou T, Wang LG, et al. 2013. Extraordinary expansion of a Sorangium cellulosum genome from an alkaline milieu. Sci. Rep. 3: 2101.   DOI
16 Park S, Lee B, Kim J, Lee C, Jang E, Cho K. 2004. Isolation and characterization of bacteriolytic wild myxobacteria. Korean J. Microbiol. Biotechnol. 32: 218-223.
17 Johnson M, Zaretskaya I, Raytselis Y, Mereshuk Y, McGinnis S, Madden TL. 2008. NCBI BLAST: a better web interface. Nucleic Acids Res. 36: W5-W9.   DOI
18 Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30: 2725-2729.   DOI
19 Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, et al. 2017. Introducing EzBioCloud: A taxonomically united database of 16S rRNA and whole genome assemblies. Int. J. Syst. Evol. Microbiol. 67: 1613-1617.   DOI
20 Pradella S, Hans A, Sproer C, Reichenbach H, Gerth K, Beyer S. 2002. Characterisation, genome size and genetic manipulation of the myxobacterium Sorangium cellulosum So ce56. Arch. Microbiol. 178: 484-492.   DOI
21 Saitou N, Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425.
22 Steinmetz H, Glaser N, Herdtweck E, Sasse F, Reichenbach H, Hofle G. 2004. Isolation, crystal and solution structure determination, and biosynthesis of tubulysins - powerful inhibitors of tubulin polymerization from myxobacteria. Angew. Chem. Int. Ed. 43: 4888-4892.   DOI
23 Hyun H, Chung J, Lee H, Youn J, Lee C, Kim D, et al. 2009. Isolation of cellulose-degrading myxobacteria Sorangium cellulosum. Korean J. Microbiol. 45: 48-53.